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The map label for this gene is thiJ

Identifier: 49478312

GI number: 49478312

Start: 3489003

End: 3489575

Strand: Reverse

Name: thiJ

Synonym: BT9727_3408

Alternate gene names: 49478312

Gene position: 3489575-3489003 (Counterclockwise)

Preceding gene: 49479445

Following gene: 49481640

Centisome position: 66.62

GC content: 34.55

Gene sequence:

>573_bases
ATGAAAAAAATACTATTATTCGTATATCCAACATTTGCGGAGTTTGAAATTACGGTAGCAACGGCATTGTTGAGAAATAA
ATATGAAATCATTACAGCAGGTTTAACAAAAGAAATGATTATAAGTGAAACAGGTTTGCAAGTACAACCACATATAGAAT
TAGGTGAAGTGCGTGTAGAAGAATATGAGGGGATTATTATTCCAGGCGGAGATGCGATACATATGGAGGATGCAGAAGTA
CTCTTTTCGGTCATTCGTCAATTTCACGAACAAGAAAAATTAGTAGCTGCTATTTGTGCTGGACCGTATGCGCTAGCAAA
AGCAGGCTTATTCAAAGAAAACTCGTATACAGTAACTATGGATTATAGAAAATTAGATTGTTTTCCAGTAGAAAATTTTG
TGTATGAAGAAGTTGTGCAACATTCAAATATTATTACAGCACAGGGGCATGCATTTGTGCCATTTGGAATAGCAATTGCC
TCTTATTTTGGAGTAACAAATGAACATAATATAAATTTTTACAGTGGTAAGGGGAATGTGATGATGGAGAAGTTTTTGCC
TGAGAATGTATAA

Upstream 100 bases:

>100_bases
ACTGGTATAAACAATTTCCTGTAAATTATGTGGACACGAATAGATTATCAGCAGACCCATTAGGGGTATATTTTATGAAG
TGGTTAAAGGGGAGAGGGCT

Downstream 100 bases:

>100_bases
AAGCTAGCTAATGCTAGCTTTTTTCTTTTTGACATCGATAAATGGCTAACGTTTTCGGATCGCGCCATATACCGTTATGA
AGTAACAATTCATTTTCTAC

Product: 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis enzyme

Products: NA

Alternate protein names: ThiJ/PfpI Family Protein; ThiJ/PfpI Domain-Containing Protein; Intracellular Protease/Amidase; Metal Dependent Phosphohydrolase; Protease; DJ-1/PfpI Family Protein; Protease I; Thij/Pfpi Family Protein; Dj-1/Pfpi Family; Intracellular Protease ThiJ/PfpI Family; ThiJ/PfpI Domain Protein

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKKILLFVYPTFAEFEITVATALLRNKYEIITAGLTKEMIISETGLQVQPHIELGEVRVEEYEGIIIPGGDAIHMEDAEV
LFSVIRQFHEQEKLVAAICAGPYALAKAGLFKENSYTVTMDYRKLDCFPVENFVYEEVVQHSNIITAQGHAFVPFGIAIA
SYFGVTNEHNINFYSGKGNVMMEKFLPENV

Sequences:

>Translated_190_residues
MKKILLFVYPTFAEFEITVATALLRNKYEIITAGLTKEMIISETGLQVQPHIELGEVRVEEYEGIIIPGGDAIHMEDAEV
LFSVIRQFHEQEKLVAAICAGPYALAKAGLFKENSYTVTMDYRKLDCFPVENFVYEEVVQHSNIITAQGHAFVPFGIAIA
SYFGVTNEHNINFYSGKGNVMMEKFLPENV
>Mature_190_residues
MKKILLFVYPTFAEFEITVATALLRNKYEIITAGLTKEMIISETGLQVQPHIELGEVRVEEYEGIIIPGGDAIHMEDAEV
LFSVIRQFHEQEKLVAAICAGPYALAKAGLFKENSYTVTMDYRKLDCFPVENFVYEEVVQHSNIITAQGHAFVPFGIAIA
SYFGVTNEHNINFYSGKGNVMMEKFLPENV

Specific function: Thiamine biosynthesis [C]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21376; Mature: 21376

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLFVYPTFAEFEITVATALLRNKYEIITAGLTKEMIISETGLQVQPHIELGEVRVE
CCEEEEEEECCCCCEEHHHHHHHHHCCEEEEEECCCHHHEEECCCCEEECCCEECEEEEE
EYEGIIIPGGDAIHMEDAEVLFSVIRQFHEQEKLVAAICAGPYALAKAGLFKENSYTVTM
CCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEEEE
DYRKLDCFPVENFVYEEVVQHSNIITAQGHAFVPFGIAIASYFGVTNEHNINFYSGKGNV
ECEEEEEEECHHHHHHHHHHHCCEEEECCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCE
MMEKFLPENV
EEECCCCCCC
>Mature Secondary Structure
MKKILLFVYPTFAEFEITVATALLRNKYEIITAGLTKEMIISETGLQVQPHIELGEVRVE
CCEEEEEEECCCCCEEHHHHHHHHHCCEEEEEECCCHHHEEECCCCEEECCCEECEEEEE
EYEGIIIPGGDAIHMEDAEVLFSVIRQFHEQEKLVAAICAGPYALAKAGLFKENSYTVTM
CCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEEEE
DYRKLDCFPVENFVYEEVVQHSNIITAQGHAFVPFGIAIASYFGVTNEHNINFYSGKGNV
ECEEEEEEECHHHHHHHHHHHCCEEEECCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCE
MMEKFLPENV
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA