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The map label for this gene is 49478302

Identifier: 49478302

GI number: 49478302

Start: 3465382

End: 3466914

Strand: Reverse

Name: 49478302

Synonym: BT9727_3386

Alternate gene names: NA

Gene position: 3466914-3465382 (Counterclockwise)

Preceding gene: 49479450

Following gene: 49479448

Centisome position: 66.19

GC content: 34.51

Gene sequence:

>1533_bases
TTGGAAGAAAGGTGGTGTAATTATTTGTTAAATGAAGGAAGGGATTGGAAAGCTATGAATAAAACAATTTTAAAAACATG
TATCATATCAGCAGTTCTAGGTGGAGTTCTATTAACTGAAAAAAATGGAGAAGTGAAAGCAGAAGAGATTACACCACAAA
CGAATCATACTGGTGTATTTAAAGATGTTCCAAAGGGGCACTGGGCATATGAGGCGATTCAGCAATTAACAGATGAGAAA
ATCGTTTTCGGATATGATGATGGGAGATTTGGTTTTGGAGATAATGTGACACGCGAACAAGTTGCAGCTTTACTGTATCG
TTATTTTAATTTGGCAGAAGAGAAAAATTATGAGAATCCATATGGGGACGTAAGTGAAGAGTCGACAAGCTATATAAAGG
AAATCTTGTCTTTAACAGAAATGGGCGTTTTTAAGGGTGACGAGCACGGGAACTTTAGACCGAAGGCATCCTTAACACGT
GCAGAAATGGCACAAGTTCTTACAAATGCTTTTCATTTAAAGGCAAAGAGTAATCATACTTTTAATGACGTTTCAACGAA
CTCATGGGCAAGCAATGCAATTAGTGCTGTTCAAACAAATAATATTGCAAAAGGAGTAGGCGGAGGGAATTTTGCTCCAA
ATATGGATGTGACGCGTGAACAGTATGCACAATTTTTATACAATGCAATACAAGAAACTGAGAAAACTCAAGAAACGAAA
GGGCAACTATTAGCTAGCATACTTGGTGAAACAAATTGGCAAGGGACAAAAGTCTATGACAAAGATCATAATGATGTAAC
GAAAGAAAATCAGAATTTCATCGGGCTTGCAAAGTATGATGCTAAAATGGCAAGGTATGAATTTTTTAATGCAAGTACAG
GAGAGAGTCGTAATGATAGCGGTACCTTTTTTATAACTAATGATGGAAAGAAAAGAGTACTAATTTCTGAGACGCAAAAT
TACCAAGCAGTTGTTGAATTAACGCAATTAGATAAAGAGAAATTTACATATAAAAGAATGGGGAAAGATGCAAAAGGGAA
CGATGTAGAAGTATTTGTTGAACATATTCCTTATCAAGAAAAAGAGCTATCTTTCACGCGACCTGATAAAAATTTAGAGA
GCTCGACGGGTAAAATAGTTACGGATGTTGATGGAGATAAAATATTATCTAGTACACTATGGAATGGAACGGTGGTATTA
GATGAACAAGGTAATAATGTAACAAAATACAATTCAAACTTAATCAGTCTAGCAAAATATGATAAAAATACAAATAAATA
TGAGTTTTTTAATGTGAATACAGGAGAAAGTCGTGGAGACTATGGTTTCTTTGATGTTGTACATGATAATAAAATAAGAG
CCCACGTTTCGTTAGGAAATAATAAATACGGTGCAGTTCTTGAACTTACAGAATTAAATAAAGAGAAATTTACGTATACA
AGAATGGGGAAAGATGCGAATGGAAAAGATATAAAAATCTTTGTTGAGCATGAACCGTATACAGGTGACCTAAAACCAAA
TTTCACAAAGTAA

Upstream 100 bases:

>100_bases
GCTATGGATACTACGAAAAAAACTTTGGAGGTGTAACACTTTGTATCCAATTAGCTGTAATGAAAATTTAAAACATGAAT
TGCTCACATAGAGTCTTCTT

Downstream 100 bases:

>100_bases
GCCTTTACTGTATGTAGCAAAGGCATTATGTTAGAAAAGGTTAGTAAGGAAAGATCAGAGTGATTATATAATTAAAATAG
TTGTTTATAGACAAATAGAT

Product: S-layer protein

Products: NA

Alternate protein names: Surface layer protein [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MEERWCNYLLNEGRDWKAMNKTILKTCIISAVLGGVLLTEKNGEVKAEEITPQTNHTGVFKDVPKGHWAYEAIQQLTDEK
IVFGYDDGRFGFGDNVTREQVAALLYRYFNLAEEKNYENPYGDVSEESTSYIKEILSLTEMGVFKGDEHGNFRPKASLTR
AEMAQVLTNAFHLKAKSNHTFNDVSTNSWASNAISAVQTNNIAKGVGGGNFAPNMDVTREQYAQFLYNAIQETEKTQETK
GQLLASILGETNWQGTKVYDKDHNDVTKENQNFIGLAKYDAKMARYEFFNASTGESRNDSGTFFITNDGKKRVLISETQN
YQAVVELTQLDKEKFTYKRMGKDAKGNDVEVFVEHIPYQEKELSFTRPDKNLESSTGKIVTDVDGDKILSSTLWNGTVVL
DEQGNNVTKYNSNLISLAKYDKNTNKYEFFNVNTGESRGDYGFFDVVHDNKIRAHVSLGNNKYGAVLELTELNKEKFTYT
RMGKDANGKDIKIFVEHEPYTGDLKPNFTK

Sequences:

>Translated_510_residues
MEERWCNYLLNEGRDWKAMNKTILKTCIISAVLGGVLLTEKNGEVKAEEITPQTNHTGVFKDVPKGHWAYEAIQQLTDEK
IVFGYDDGRFGFGDNVTREQVAALLYRYFNLAEEKNYENPYGDVSEESTSYIKEILSLTEMGVFKGDEHGNFRPKASLTR
AEMAQVLTNAFHLKAKSNHTFNDVSTNSWASNAISAVQTNNIAKGVGGGNFAPNMDVTREQYAQFLYNAIQETEKTQETK
GQLLASILGETNWQGTKVYDKDHNDVTKENQNFIGLAKYDAKMARYEFFNASTGESRNDSGTFFITNDGKKRVLISETQN
YQAVVELTQLDKEKFTYKRMGKDAKGNDVEVFVEHIPYQEKELSFTRPDKNLESSTGKIVTDVDGDKILSSTLWNGTVVL
DEQGNNVTKYNSNLISLAKYDKNTNKYEFFNVNTGESRGDYGFFDVVHDNKIRAHVSLGNNKYGAVLELTELNKEKFTYT
RMGKDANGKDIKIFVEHEPYTGDLKPNFTK
>Mature_510_residues
MEERWCNYLLNEGRDWKAMNKTILKTCIISAVLGGVLLTEKNGEVKAEEITPQTNHTGVFKDVPKGHWAYEAIQQLTDEK
IVFGYDDGRFGFGDNVTREQVAALLYRYFNLAEEKNYENPYGDVSEESTSYIKEILSLTEMGVFKGDEHGNFRPKASLTR
AEMAQVLTNAFHLKAKSNHTFNDVSTNSWASNAISAVQTNNIAKGVGGGNFAPNMDVTREQYAQFLYNAIQETEKTQETK
GQLLASILGETNWQGTKVYDKDHNDVTKENQNFIGLAKYDAKMARYEFFNASTGESRNDSGTFFITNDGKKRVLISETQN
YQAVVELTQLDKEKFTYKRMGKDAKGNDVEVFVEHIPYQEKELSFTRPDKNLESSTGKIVTDVDGDKILSSTLWNGTVVL
DEQGNNVTKYNSNLISLAKYDKNTNKYEFFNVNTGESRGDYGFFDVVHDNKIRAHVSLGNNKYGAVLELTELNKEKFTYT
RMGKDANGKDIKIFVEHEPYTGDLKPNFTK

Specific function: The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted, cell wall, S-layer [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003343
- InterPro:   IPR001119 [H]

Pfam domain/function: PF02368 Big_2; PF00395 SLH [H]

EC number: NA

Molecular weight: Translated: 57744; Mature: 57744

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERWCNYLLNEGRDWKAMNKTILKTCIISAVLGGVLLTEKNGEVKAEEITPQTNHTGVF
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCCCCCCCC
KDVPKGHWAYEAIQQLTDEKIVFGYDDGRFGFGDNVTREQVAALLYRYFNLAEEKNYENP
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
YGDVSEESTSYIKEILSLTEMGVFKGDEHGNFRPKASLTRAEMAQVLTNAFHLKAKSNHT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
FNDVSTNSWASNAISAVQTNNIAKGVGGGNFAPNMDVTREQYAQFLYNAIQETEKTQETK
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQLLASILGETNWQGTKVYDKDHNDVTKENQNFIGLAKYDAKMARYEFFNASTGESRNDS
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHEEECCCCCCCCCCC
GTFFITNDGKKRVLISETQNYQAVVELTQLDKEKFTYKRMGKDAKGNDVEVFVEHIPYQE
CEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEHEECCCCCC
KELSFTRPDKNLESSTGKIVTDVDGDKILSSTLWNGTVVLDEQGNNVTKYNSNLISLAKY
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEEECCCCCEEEECCCEEEEEEC
DKNTNKYEFFNVNTGESRGDYGFFDVVHDNKIRAHVSLGNNKYGAVLELTELNKEKFTYT
CCCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEEECCCCHHEEEE
RMGKDANGKDIKIFVEHEPYTGDLKPNFTK
ECCCCCCCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEERWCNYLLNEGRDWKAMNKTILKTCIISAVLGGVLLTEKNGEVKAEEITPQTNHTGVF
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCCCCCCCC
KDVPKGHWAYEAIQQLTDEKIVFGYDDGRFGFGDNVTREQVAALLYRYFNLAEEKNYENP
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
YGDVSEESTSYIKEILSLTEMGVFKGDEHGNFRPKASLTRAEMAQVLTNAFHLKAKSNHT
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
FNDVSTNSWASNAISAVQTNNIAKGVGGGNFAPNMDVTREQYAQFLYNAIQETEKTQETK
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQLLASILGETNWQGTKVYDKDHNDVTKENQNFIGLAKYDAKMARYEFFNASTGESRNDS
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHEEECCCCCCCCCCC
GTFFITNDGKKRVLISETQNYQAVVELTQLDKEKFTYKRMGKDAKGNDVEVFVEHIPYQE
CEEEEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEHEECCCCCC
KELSFTRPDKNLESSTGKIVTDVDGDKILSSTLWNGTVVLDEQGNNVTKYNSNLISLAKY
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEEECCCCCEEEECCCEEEEEEC
DKNTNKYEFFNVNTGESRGDYGFFDVVHDNKIRAHVSLGNNKYGAVLELTELNKEKFTYT
CCCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEEECCCCHHEEEE
RMGKDANGKDIKIFVEHEPYTGDLKPNFTK
ECCCCCCCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8964497 [H]