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The map label for this gene is dctA

Identifier: 49478283

GI number: 49478283

Start: 3424396

End: 3425712

Strand: Reverse

Name: dctA

Synonym: BT9727_3346

Alternate gene names: 49478283

Gene position: 3425712-3424396 (Counterclockwise)

Preceding gene: 49478970

Following gene: 49478282

Centisome position: 65.41

GC content: 34.78

Gene sequence:

>1317_bases
ATGTTATTGATGAAAATAGTTTGGGAGAAACGAAAAAACAAGGGAGTATTAAAAATGAGAATTGTAAAAAATTTAACATT
CCAAGTTATTGTGGCCATTATTTGTGGTATTGCAGTAGGAGCAATTTGGCCGAGTGTTGGACAAGAGATGAAGCCAATTG
GTGAAACATTCATCAATATGATTAAAATGGTTATTGCACCAATTATCTTTTTAACAATTGTGCTTGGTATTGCAAGTATG
GGAAGTATGAAAAAGGTAGGTCGTGTTGGCGGGAAGGCACTATTATATTTTGAAATTGTTACAACAGCGGCTTTAATTAT
TGGTATCATCGTAGCGAATGTTGTGCGTCCAGGAGACGGATTAGACCCTTCAAAACTAAAGGGCGGAGATGTTTCACAAT
ATGTCCAAAGTGGTCAAGAAATGAAATGGATGGATTTCTTTTTACATATTGTGCCGTCTAACATGTTTGAAGCATTTGCA
AAAGGTGATATTTTACAAGTGTTATTTTTCTCTATTTTATTTGGCGCAGGGTTAACGATGTTAGGGAAAAATGGACAGCC
GGTAATCGACTTTTTTGAAAGATTATCGAAAGTATTTTTTAACATTTTATCAATTGTAATGAAATTAGCACCGGTTGGAG
CATTCGGTGGAATGGCATTTACAATTGGAAAATACGGTTTAAGCACGCTCATACCACTTGGTAAACTAATGATTTGTGTA
TATGCGACAATGGCATTATTTGTTTTTATTGTGTTAAATTTCATTTGTAAACTGTACAAATTTAGTTTGTGGAAATATTT
AGCGCATATTAAAGAAGAATTACTTATTGTTCTTGGGACATCATCATCAGAATCAGTATTGCCGCGAATGATGACAAAGA
TGGAGGACTTCGGATGTTCGAAGCCAGTAGTAGGTTTAGTCATTCCGACAGGATATTCTTTCAATTTAGATGGAACAACG
ATTTATTTATCCATGGCTACTATATTTTTAGCTCAAGTCTTTCACGTCGATCTTTCACTAGGTCAACAATTAACTATAAT
AGCTATTTTGTTAGTTACATCTAAAGGAGCAGCCGCAGTAACAGGTGGAGGTTTCATTGTACTAGCATCTACTCTATCTG
CAATGAATGTTATTCCATTAGAAGGGTTAGCACTATTACTTGGTGTAGATCGTTTCATGTCAGAAGCACGTGCTATCGTT
AATTTAATTGGTAATGGTATTGCGACTGTAGTAGTTGCAAAAAGTGAAAATGAATTTGATAATGAGAAGTATATGAAAGT
ACTAGAAGAAATGAAAAGAGAGAAAATGGCCGGATAA

Upstream 100 bases:

>100_bases
TTATCATTAAATGTTATATACTATTTTCAAAATTTATATAATAGTGTAACATAAAGTTAAGGTTTAAGTTACATAACAGG
GGGGCCATCACATAGAATAA

Downstream 100 bases:

>100_bases
AAAGTCAGCTTTGCTGACTTTTTTATTTTTGTAGAAAAGAGGTTTGTTATAATATACATTATTTATTAACAATAATTTAC
AGGTATTTTGTAGAGAAGGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLLMKIVWEKRKNKGVLKMRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASM
GSMKKVGRVGGKALLYFEIVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFA
KGDILQVLFFSILFGAGLTMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICV
YATMALFVFIVLNFICKLYKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTT
IYLSMATIFLAQVFHVDLSLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIV
NLIGNGIATVVVAKSENEFDNEKYMKVLEEMKREKMAG

Sequences:

>Translated_438_residues
MLLMKIVWEKRKNKGVLKMRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASM
GSMKKVGRVGGKALLYFEIVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFA
KGDILQVLFFSILFGAGLTMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICV
YATMALFVFIVLNFICKLYKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTT
IYLSMATIFLAQVFHVDLSLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIV
NLIGNGIATVVVAKSENEFDNEKYMKVLEEMKREKMAG
>Mature_438_residues
MLLMKIVWEKRKNKGVLKMRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASM
GSMKKVGRVGGKALLYFEIVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFA
KGDILQVLFFSILFGAGLTMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICV
YATMALFVFIVLNFICKLYKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTT
IYLSMATIFLAQVFHVDLSLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIV
NLIGNGIATVVVAKSENEFDNEKYMKVLEEMKREKMAG

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.7216035634744, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=25.9860788863109, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI40254478, Length=478, Percent_Identity=23.8493723849372, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5032093, Length=421, Percent_Identity=26.1282660332542, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI66773030, Length=478, Percent_Identity=25.3138075313808, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI223468564, Length=283, Percent_Identity=28.6219081272085, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=28.6219081272085, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194239697, Length=432, Percent_Identity=24.537037037037, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI4827012, Length=439, Percent_Identity=25.0569476082005, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=24.2021276595745, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI301601644, Length=176, Percent_Identity=28.4090909090909, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=55.2884615384615, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790514, Length=430, Percent_Identity=40.4651162790698, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1789473, Length=319, Percent_Identity=23.1974921630094, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=425, Percent_Identity=26.8235294117647, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71983099, Length=475, Percent_Identity=25.0526315789474, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=422, Percent_Identity=25.1184834123223, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=406, Percent_Identity=26.8472906403941, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=413, Percent_Identity=25.90799031477, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=24.9427917620137, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=23.6238532110092, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583025, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17137668, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24583023, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281360481, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281360483, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47707; Mature: 47707

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLMKIVWEKRKNKGVLKMRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINM
CHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
IKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFEIVTTAALIIGIIVANVVRPGDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGLTM
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHE
LGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICV
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YATMALFVFIVLNFICKLYKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCC
KPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDLSLGQQLTIIAILLVTSKGAAAV
CCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEE
TGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENEFD
ECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
NEKYMKVLEEMKREKMAG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]