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The map label for this gene is moaA

Identifier: 49478276

GI number: 49478276

Start: 3399099

End: 3400115

Strand: Reverse

Name: moaA

Synonym: BT9727_3327

Alternate gene names: 49478276

Gene position: 3400115-3399099 (Counterclockwise)

Preceding gene: 49478277

Following gene: 49478971

Centisome position: 64.92

GC content: 36.48

Gene sequence:

>1017_bases
ATGAAATCTGTCACATTAGACAAACTACAACGTCCGTTAAAGGATTTACGTATTTCAGTTACTGATCGCTGTAATTTTCG
CTGTCGATATTGTATGCCAGAAGAAATATTTGGTCCGGATTACTCGTTTTTGTCTAATGATAAAATTTTATCTTTTGATG
AAATTGAAAGAATAACGCGTATTTTCGTTTCTTTAGGTGTTAGAAAATTACGGATTACAGGCGGAGAACCGTTACTTCGA
AGAGGTCTTCCGCAGCTTATCGAGCGCCTTAATAAAATAGATGGTGTAGAGGATATCGGCTTAACAACCAATGGATCGTT
ACTTAAAAAGTTTGCTCCTGATTTATATAAGGCGGGTTTATCACGTGTGACAGTTAGTTTAGATTCCTTAGAAGAAGAGC
GGTTTTTCTATTTAAATGGTAATAGAAGCAAAGTACAAAGGGTTCTGGAAGGCATACAAGCTGCAGCTGAAGTTGGAATG
AAAATTAAAATAAACATGGTCGTTCAAAAGGGGAAAAACGAACAGGACATCTTGCAGATGGCGCAATACTTTAAGGAAAA
CAAGCATATTCTTCGTTTTATTGAGTATATGGACGTTGGAAATTATAACGGTTGGGAGTTAAAAGAGGTCGTATCTAAAC
AAGAAATAGTAGATATGATTCATCAAGTTATGCCATTAGAACGGATAGAAGCGAATTATGCAGGTGAGGTTGCAACTCGC
TATCGTTATATTGGAAGTGATGAAGAAGTAGGTGTTATTTCATCAGTAACAGATTCCTTCTGTTCGTCATGTACGAGAGC
TCGTATTTCTGCAGAAGGAAAATTATATACTTGTTTATTCGCTTCTAAAGGAAATGACTTGAGAGAACTGCTTCGATCTG
ACTACACTGACGAGGAAATAACATATGTCGTACGCGACATATGGAACAACCGAGAAGATCGTTATTCAGATGAACGCTTA
AGTAACAGTAATAAAAAAGTTATCCCTAAAATTGAAATGTCACATATCGGTGGTTAA

Upstream 100 bases:

>100_bases
GTATTACTACGATAGGATTTATTCGGAATCAATCCTTAAATGTATATACGCACCCAGAGCGTGTTTTAAATATAAAATAA
GTAAAAGCGAGGCAATAAAT

Downstream 100 bases:

>100_bases
TATAAAGTTGACATAAAAGTGAAAAATGGAAGGTGTACAAGAATTGTAGATTTTCTATTTTATAGTAAAAGTGATGATTG
ATCTCTCTGTAAAAGATGTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKSVTLDKLQRPLKDLRISVTDRCNFRCRYCMPEEIFGPDYSFLSNDKILSFDEIERITRIFVSLGVRKLRITGGEPLLR
RGLPQLIERLNKIDGVEDIGLTTNGSLLKKFAPDLYKAGLSRVTVSLDSLEEERFFYLNGNRSKVQRVLEGIQAAAEVGM
KIKINMVVQKGKNEQDILQMAQYFKENKHILRFIEYMDVGNYNGWELKEVVSKQEIVDMIHQVMPLERIEANYAGEVATR
YRYIGSDEEVGVISSVTDSFCSSCTRARISAEGKLYTCLFASKGNDLRELLRSDYTDEEITYVVRDIWNNREDRYSDERL
SNSNKKVIPKIEMSHIGG

Sequences:

>Translated_338_residues
MKSVTLDKLQRPLKDLRISVTDRCNFRCRYCMPEEIFGPDYSFLSNDKILSFDEIERITRIFVSLGVRKLRITGGEPLLR
RGLPQLIERLNKIDGVEDIGLTTNGSLLKKFAPDLYKAGLSRVTVSLDSLEEERFFYLNGNRSKVQRVLEGIQAAAEVGM
KIKINMVVQKGKNEQDILQMAQYFKENKHILRFIEYMDVGNYNGWELKEVVSKQEIVDMIHQVMPLERIEANYAGEVATR
YRYIGSDEEVGVISSVTDSFCSSCTRARISAEGKLYTCLFASKGNDLRELLRSDYTDEEITYVVRDIWNNREDRYSDERL
SNSNKKVIPKIEMSHIGG
>Mature_338_residues
MKSVTLDKLQRPLKDLRISVTDRCNFRCRYCMPEEIFGPDYSFLSNDKILSFDEIERITRIFVSLGVRKLRITGGEPLLR
RGLPQLIERLNKIDGVEDIGLTTNGSLLKKFAPDLYKAGLSRVTVSLDSLEEERFFYLNGNRSKVQRVLEGIQAAAEVGM
KIKINMVVQKGKNEQDILQMAQYFKENKHILRFIEYMDVGNYNGWELKEVVSKQEIVDMIHQVMPLERIEANYAGEVATR
YRYIGSDEEVGVISSVTDSFCSSCTRARISAEGKLYTCLFASKGNDLRELLRSDYTDEEITYVVRDIWNNREDRYSDERL
SNSNKKVIPKIEMSHIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=300, Percent_Identity=36.3333333333333, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI22027536, Length=300, Percent_Identity=36.3333333333333, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI1786999, Length=307, Percent_Identity=35.8306188925081, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32566329, Length=335, Percent_Identity=32.8358208955224, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=316, Percent_Identity=32.9113924050633, Blast_Score=193, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17561210, Length=335, Percent_Identity=33.4328358208955, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI28574488, Length=297, Percent_Identity=35.6902356902357, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI28574490, Length=293, Percent_Identity=35.8361774744027, Blast_Score=193, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 38917; Mature: 38917

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVTLDKLQRPLKDLRISVTDRCNFRCRYCMPEEIFGPDYSFLSNDKILSFDEIERITR
CCCCCHHHHHHHHHHCEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHH
IFVSLGVRKLRITGGEPLLRRGLPQLIERLNKIDGVEDIGLTTNGSLLKKFAPDLYKAGL
HHHHHCCEEEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHCCH
SRVTVSLDSLEEERFFYLNGNRSKVQRVLEGIQAAAEVGMKIKINMVVQKGKNEQDILQM
HHEEHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHH
AQYFKENKHILRFIEYMDVGNYNGWELKEVVSKQEIVDMIHQVMPLERIEANYAGEVATR
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
YRYIGSDEEVGVISSVTDSFCSSCTRARISAEGKLYTCLFASKGNDLRELLRSDYTDEEI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCHHH
TYVVRDIWNNREDRYSDERLSNSNKKVIPKIEMSHIGG
HHHHHHHHCCCCCCCCHHHHCCCCCEECCEECCCCCCC
>Mature Secondary Structure
MKSVTLDKLQRPLKDLRISVTDRCNFRCRYCMPEEIFGPDYSFLSNDKILSFDEIERITR
CCCCCHHHHHHHHHHCEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHH
IFVSLGVRKLRITGGEPLLRRGLPQLIERLNKIDGVEDIGLTTNGSLLKKFAPDLYKAGL
HHHHHCCEEEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHCCH
SRVTVSLDSLEEERFFYLNGNRSKVQRVLEGIQAAAEVGMKIKINMVVQKGKNEQDILQM
HHEEHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHH
AQYFKENKHILRFIEYMDVGNYNGWELKEVVSKQEIVDMIHQVMPLERIEANYAGEVATR
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
YRYIGSDEEVGVISSVTDSFCSSCTRARISAEGKLYTCLFASKGNDLRELLRSDYTDEEI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCHHH
TYVVRDIWNNREDRYSDERLSNSNKKVIPKIEMSHIGG
HHHHHHHHCCCCCCCCHHHHCCCCCEECCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA