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The map label for this gene is amyS

Identifier: 49478255

GI number: 49478255

Start: 3326056

End: 3327597

Strand: Reverse

Name: amyS

Synonym: BT9727_3261

Alternate gene names: 49478255

Gene position: 3327597-3326056 (Counterclockwise)

Preceding gene: 49479259

Following gene: 49478253

Centisome position: 63.53

GC content: 36.9

Gene sequence:

>1542_bases
ATGTTTAAAAAAATAACAATAGTCGGCTTGTCGGTTGTTTTGTTTTTACCTAGTATATATGGGGGGAGTAAAGTGTATGC
AGATACGATTAACAATGGAACGTTAATGCAGTATTTTGAGTGGTATGCTCCAAGTGATGGGAATCATTGGAATCGTTTGC
GCACTGATGCTGAAAATTTAGCGCAAAAAGGAATTACATCTGTTTGGATACCTCCTGCGTATAAAGGAACTACGCAAAAT
GATGTAGGATATGGAGCATATGATTTATATGATTTGGGTGAATTCAATCAAAAGGGAACAGTGCGGACGAAATATGGGAC
GAAAGCACAATTGAAATCTGCAATTGAAGCTTTACATAAGCAAAACATCGATGTATACGGCGATGTAGTTATGAATCATA
AAGGTGGAGCAGATTATACGGAAACTGTAACAGCTGTTGAGGTAGACCGTAACAATCGAAATGTTGAGGTATCAGGTGAT
TATGAAATTAGTGCATGGACAGGGTTTAACTTTCCTGGACGTGGAGATAATTATTCTAATTTCAAATGGAAATGGTATCA
TTTTGACGGAACGGATTGGGATGAAGGAAGGAAATTAAATCGAATTTATAAATTTAGGGGTATAGGTAAAGCATGGGACT
GGGAAGTGTCTAGTGAGAATGGAAATTATGATTATTTGATGTATGCAGACCTTGATTTTGATCATCCAGATGTTGCGAAT
GAGATGAAAAATTGGGGGACGTGGTATGCGAATGAATTAAATTTAGATGGCTTTCGTTTAGATGCTGTTAAACATATTGA
TCATGAATATTTACGCGATTGGGTAAATCATGTTAGGCAGCAAACGAGGAAAGAAATGTTTACGGTGGCTGAATATTGGC
AAAATGATATCCACACTTTAAATAATTATTTAGCGAAAGTCAATTATAATCAATCTGTATTTGATGCACCACTGCATTAC
AATTTTCATTATGCTTCAAAAGGAAATGGGAATTATGATATGAGAAATATTTTAAATGGAACAGTAATGCAAAATCATCC
TGCACTCGCAGTTACTCTTGTTGAGAATCATGATTCTCAGCCTGGTCAGTCATTGGAATCTGTAGTAAGTGCGTGGTTTA
AACCGTTGGCATATGGATTTATTTTAACTCGTGCAGAGGGATATCCTTCAGTTTTTTATGGTGATTACTATGGGACAAGC
GGAAATAGTAGTTATGAAATTCCAGCGTTAAAAGATAAAATTGATCCGATTTTGACAGCACGAAAAAACTTTGCATATGG
TACGCAGCGTGATTATTTAGACCATCCAGATGTGATTGGATGGACAAGAGAAGGTGATAGTGTACATGCTAATTCTGGTT
TAGCAACATTAATCTCTGATGGACCGGGAGGATCAAAGTGGATGGATGTTGGAAAGAATAATGCAGGGGAAGTATGGCAC
GATATGACGGGTAATCAAACAAATACTGTAACAATTAATAAGGATGGATGGGGGCAATTCCATGTAAGTGGAGGATCAGT
TTCCATATATGTTCAGCAGTGA

Upstream 100 bases:

>100_bases
TGGAAGAGTGAAAGATTGATACTTTACTAACAGAGGGACGAATATTATAATAATTCTGATAAACTATGCAAACGCTTTCT
TAAAAGGGGATGGTATACAG

Downstream 100 bases:

>100_bases
ATTGTTAATAGAAAAGAAGGGGTAGACTGAAATATAAGTTTTCCTCTTCTTTTTATATTGTTATCATTCCTAAATCGTTA
TTTTAATAAATGAACTAGTC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase; BLA [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MFKKITIVGLSVVLFLPSIYGGSKVYADTINNGTLMQYFEWYAPSDGNHWNRLRTDAENLAQKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIEALHKQNIDVYGDVVMNHKGGADYTETVTAVEVDRNNRNVEVSGD
YEISAWTGFNFPGRGDNYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKNWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTRKEMFTVAEYWQNDIHTLNNYLAKVNYNQSVFDAPLHY
NFHYASKGNGNYDMRNILNGTVMQNHPALAVTLVENHDSQPGQSLESVVSAWFKPLAYGFILTRAEGYPSVFYGDYYGTS
GNSSYEIPALKDKIDPILTARKNFAYGTQRDYLDHPDVIGWTREGDSVHANSGLATLISDGPGGSKWMDVGKNNAGEVWH
DMTGNQTNTVTINKDGWGQFHVSGGSVSIYVQQ

Sequences:

>Translated_513_residues
MFKKITIVGLSVVLFLPSIYGGSKVYADTINNGTLMQYFEWYAPSDGNHWNRLRTDAENLAQKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIEALHKQNIDVYGDVVMNHKGGADYTETVTAVEVDRNNRNVEVSGD
YEISAWTGFNFPGRGDNYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKNWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTRKEMFTVAEYWQNDIHTLNNYLAKVNYNQSVFDAPLHY
NFHYASKGNGNYDMRNILNGTVMQNHPALAVTLVENHDSQPGQSLESVVSAWFKPLAYGFILTRAEGYPSVFYGDYYGTS
GNSSYEIPALKDKIDPILTARKNFAYGTQRDYLDHPDVIGWTREGDSVHANSGLATLISDGPGGSKWMDVGKNNAGEVWH
DMTGNQTNTVTINKDGWGQFHVSGGSVSIYVQQ
>Mature_513_residues
MFKKITIVGLSVVLFLPSIYGGSKVYADTINNGTLMQYFEWYAPSDGNHWNRLRTDAENLAQKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIEALHKQNIDVYGDVVMNHKGGADYTETVTAVEVDRNNRNVEVSGD
YEISAWTGFNFPGRGDNYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKNWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTRKEMFTVAEYWQNDIHTLNNYLAKVNYNQSVFDAPLHY
NFHYASKGNGNYDMRNILNGTVMQNHPALAVTLVENHDSQPGQSLESVVSAWFKPLAYGFILTRAEGYPSVFYGDYYGTS
GNSSYEIPALKDKIDPILTARKNFAYGTQRDYLDHPDVIGWTREGDSVHANSGLATLISDGPGGSKWMDVGKNNAGEVWH
DMTGNQTNTVTINKDGWGQFHVSGGSVSIYVQQ

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=492, Percent_Identity=41.260162601626, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 58343; Mature: 58343

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKITIVGLSVVLFLPSIYGGSKVYADTINNGTLMQYFEWYAPSDGNHWNRLRTDAENL
CCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHCCCCCCCCHHHHHCCHHHH
AQKGITSVWIPPAYKGTTQNDVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIEALHK
HHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHH
QNIDVYGDVVMNHKGGADYTETVTAVEVDRNNRNVEVSGDYEISAWTGFNFPGRGDNYSN
CCCCEEEEEEEECCCCCCHHHEEEEEEEECCCCEEEECCCEEEEEEECCCCCCCCCCCCC
FKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
CEEEEEECCCCCHHCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHH
EMKNWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTRKEMFTVAEYWQNDIHTL
HHHHCCCEEEEEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNYLAKVNYNQSVFDAPLHYNFHYASKGNGNYDMRNILNGTVMQNHPALAVTLVENHDSQ
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCEEECCCCEEEEEEECCCCC
PGQSLESVVSAWFKPLAYGFILTRAEGYPSVFYGDYYGTSGNSSYEIPALKDKIDPILTA
CCHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCEECCCCCCCEECCHHHHHHHHHHHH
RKNFAYGTQRDYLDHPDVIGWTREGDSVHANSGLATLISDGPGGSKWMDVGKNNAGEVWH
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCHHHCCCCCCCCCCHH
DMTGNQTNTVTINKDGWGQFHVSGGSVSIYVQQ
CCCCCCCEEEEEECCCCEEEEEECCEEEEEEEC
>Mature Secondary Structure
MFKKITIVGLSVVLFLPSIYGGSKVYADTINNGTLMQYFEWYAPSDGNHWNRLRTDAENL
CCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHCCCCCCCCHHHHHCCHHHH
AQKGITSVWIPPAYKGTTQNDVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIEALHK
HHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHH
QNIDVYGDVVMNHKGGADYTETVTAVEVDRNNRNVEVSGDYEISAWTGFNFPGRGDNYSN
CCCCEEEEEEEECCCCCCHHHEEEEEEEECCCCEEEECCCEEEEEEECCCCCCCCCCCCC
FKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
CEEEEEECCCCCHHCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHH
EMKNWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTRKEMFTVAEYWQNDIHTL
HHHHCCCEEEEEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNYLAKVNYNQSVFDAPLHYNFHYASKGNGNYDMRNILNGTVMQNHPALAVTLVENHDSQ
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCEEECCCCEEEEEEECCCCC
PGQSLESVVSAWFKPLAYGFILTRAEGYPSVFYGDYYGTSGNSSYEIPALKDKIDPILTA
CCHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCEECCCCCCCEECCHHHHHHHHHHHH
RKNFAYGTQRDYLDHPDVIGWTREGDSVHANSGLATLISDGPGGSKWMDVGKNNAGEVWH
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCHHHCCCCCCCCCCHH
DMTGNQTNTVTINKDGWGQFHVSGGSVSIYVQQ
CCCCCCCEEEEEECCCCEEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2418011; 3009417; 14632998; 6334606; 6609154; 2265757; 2540150; 6172418; 11997021; 2394736; 8771184; 10966804; 12736372; 12915728; 7877175; 9551551; 10924103; 12540849 [H]