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The map label for this gene is ywqM [H]

Identifier: 49478252

GI number: 49478252

Start: 3319204

End: 3320097

Strand: Reverse

Name: ywqM [H]

Synonym: BT9727_3255

Alternate gene names: 49478252

Gene position: 3320097-3319204 (Counterclockwise)

Preceding gene: 49478985

Following gene: 49479313

Centisome position: 63.39

GC content: 35.79

Gene sequence:

>894_bases
ATGGAGATAAAACAATTAATTACCTTTAAAATAGCCGCGGATACTTTGAACTTTACACAAACTGCGAAGAAATTAAACTT
TGCTCAATCGAGCGTAACGGCGCAAATTAAAACGTTAGAAGCTGAGCTAGGTACGCCGTTATTTGAAAGATTAGGAAAAC
GTCTTTTCTTAACTGAAGCAGGGAGGAAGTTTCAATTATATGCTGATAAGATGATTGGACTTAGTAACGAAGCGAAAATG
GCTGTGAAAGATGATGAAGAAATAGCTGGTACGTTAACGATTGGTGCACAAGAAAGTCAGTGTACATATAGGCTTCCATC
TATATTAAAGAGATTTAAAGCACAATTTCCTCAAATAAAACTTATATTTAAACCAGCACATTCTAATAAAGATGCGAAGG
AACAATTAATGGAGGGAAAAGTCGATCTTGCATTTATTTTGGATGAATGTAAAACAGAAGATGCTTTACATGTGGAGCCG
CTTATGAAAGAAGAATTAAAAGTAGTAGTTGCTCCAGGGCATCGTTTACTGGAGCAAACTTCCGTTTCTATAAAAGATTT
AGAGAAGGAAACACTTTTATTAACAGAACTAGGGTGCTCATATCGGACTTTATTTGAGGAGTTATTTCGTGCGAAAGACA
TATATCCAGCAAATAAGATTGAGTTTGTTAGTGTCGAGGCAATTAAACAATGCGTCATTGCAGATTTAGGTGTTGCTGTG
TTACCTGCTATAGTAGTAGAAAAAGATATGCGAGAAGGGACGATAAAGGAGTTAGTTTTAAAAGAAACAATTCCTCCGAT
TTATACGCAAATCGCTTGGCATAAAGACAAATGGATGACAGTGCCACTGCAGCAATTTATTGATGTAACAAGAGAGTTTT
TTACAACGGATTAA

Upstream 100 bases:

>100_bases
TTTGTCGATGCTCTTTATCATACAATACAAATATCAATAGACAAAGAGGATTATTTTGATAAGATCAATCGAAAATTTCG
ATTGAAAAGAGGGATGATAA

Downstream 100 bases:

>100_bases
GTGGAGAAATATTTGTTTTCTATAAAAGAAAAAATAGCAATTTTGTATAAAGAACCGATGCTTGCATGTAACAAGTATCG
GTTCTTTTATGTTTGAAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVVAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCVIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD

Sequences:

>Translated_297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVVAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCVIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
>Mature_297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVVAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCVIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=28.3783783783784, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=29.757785467128, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790262, Length=267, Percent_Identity=27.3408239700375, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=24.9146757679181, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=28.125, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=26.171875, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=25.8196721311475, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=27.3076923076923, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=26.4, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=25.1700680272109, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=21.3559322033898, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=25.5060728744939, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788706, Length=257, Percent_Identity=24.9027237354086, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=24.4224422442244, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788626, Length=253, Percent_Identity=25.6916996047431, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=26.8774703557312, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33715; Mature: 33715

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEA
CCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECC
GRKFQLYADKMIGLSNEAKMAVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIK
CCEEEEEHHHHHCCCCCCCEEECCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEPLMKEELKVVVAPGHRLLEQT
EEEECCCCCCHHHHHHHCCCCHHEEHHHHHCCCCCEECCHHHHHCCEEEECCCHHHHHHH
SVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCVIADLGVAV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEA
CCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECC
GRKFQLYADKMIGLSNEAKMAVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIK
CCEEEEEHHHHHCCCCCCCEEECCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEPLMKEELKVVVAPGHRLLEQT
EEEECCCCCCHHHHHHHCCCCHHEEHHHHHCCCCCEECCHHHHHCCEEEECCCHHHHHHH
SVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCVIADLGVAV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]