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The map label for this gene is phrB

Identifier: 49478047

GI number: 49478047

Start: 3010377

End: 3011807

Strand: Reverse

Name: phrB

Synonym: BT9727_2935

Alternate gene names: 49478047

Gene position: 3011807-3010377 (Counterclockwise)

Preceding gene: 49479082

Following gene: 49478046

Centisome position: 57.5

GC content: 35.22

Gene sequence:

>1431_bases
ATGCAAAATAAAATTATCGTTATGTTTCAAAAAGATTTTCGCTTATATGATAACCCAGCTCTATTTGAAGCGGCTCAGTC
CGGTGAGGTTGTTCCGGTATATGTACATGATGAAACTTTTTCAATGGGAAGTGCGTCAAAGTGGTGGTTACACCATGCAA
TAATAGATGTAAAGAAGCAACTTGAGGCATTGGGCTCTACTTTAATCATTCGTAAAGGAAGTACGCAAGAAGAAATACTT
TCTCTCGTAGAACAGTTAGGTATAACGGCTGTATATTGGAATATTTGTTATGATCCGGACAGATTACAATTTAATCAAAA
AATGAAAATGATGTTAGAACATAAAGGTATGATCTGTAAGGAATTTAATTCACATTTATTATTAGAGCCTTGGGTTATTA
AAAAGAAAGATAACACTGAATATAAGGTGTTTACGCCTTTTTACAATGCATTTCAAAAGCAGGTAATACATAAGCCTATT
AGTAAAGTGCAGAGTATAAAGGGAGGAAACTCTTTACCAGTAAGCTTATCTGTTTCAGAATTACACTTGTTGCCGACTAT
ACCGTGGACATCTCATATGGAATCAATATGGGAGCCTACAGAAGAAGGGGCATACAAAACATGGAAGGAATTTTTCTCTA
GCAAATTGGCCTCTTATAGTGAAGGAAGAGATTTTCCAAATCAAAATGCTCATTCGATGTTGGCACCTTATCTTTCATTT
GGTCAAATATCAGTCAAACTAATCTATCATTACTTAATAAATAAAAGTACAGAAAGCCAATGTAGTCTTTTTGAAAAACA
AGTAAATAGTTTTATACGTCAATTAATTTGGCGAGAGTTTTCTTATTATTTGCTATATCATTATCCGTTTACAGCATATA
AACCTCTTAATAAGAGCTTTGAACATTTTCCGTGGAATAATGAAGAGGAGTTATTAAGAGTATGGCAGAAAGGTGACACT
GGTTATCCGTTTATTGATGCAGGAATGAGGGAACTGTGGCAAACAGGTTTTATGCATAATCGCACAAGAATGGCTGTAGC
CTCTTTTCTTGTAAAGCATTTGTTAATTCCGTGGCAAGAAGGAGCAAAATGGTTTATGGATACACTATTAGATGCTGATA
TTGCAAATAATACAATGGGGTGGCAATGGGTTGCTGGAAGTGGAGCAGATGCATCACCATACTTTCGTATTTTTAATCCG
ATCACACAAGGAGAAAAGTTTGATAAAAACGGAGAGTATATAAGAGAATGGGTACCAGAATTAAAAGATATGCCTAATAA
ATATATACATAAACCGTGGGAAGCACCTGAGCATATTTTACAAAAGGCCAATATACAGCTTGGTCATACATATCCTTTGC
CAGTCGTTGATCATAAGGCAGCACGAGAGAGAGCGCTTTGTGCATATAAAAGTATGAAAGAATTCGTATGA

Upstream 100 bases:

>100_bases
TACCCCCACCTCAAAAATCAACGGAAGCAAAGAAGTTAGGTGGGGATCGGGTTGATTAAAGTTTCACTTTATTATGTTTT
AAGAGTAAGAAAGGATAGCC

Downstream 100 bases:

>100_bases
AATAGGTTAAACAATATGTATTTGGAATTTAAATATTAGAATTTTATCCATTACAAGGACATACTAATCATATAAAGTTT
CTAAAACCTTCTATTGAAAA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSMGSASKWWLHHAIIDVKKQLEALGSTLIIRKGSTQEEIL
SLVEQLGITAVYWNICYDPDRLQFNQKMKMMLEHKGMICKEFNSHLLLEPWVIKKKDNTEYKVFTPFYNAFQKQVIHKPI
SKVQSIKGGNSLPVSLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTWKEFFSSKLASYSEGRDFPNQNAHSMLAPYLSF
GQISVKLIYHYLINKSTESQCSLFEKQVNSFIRQLIWREFSYYLLYHYPFTAYKPLNKSFEHFPWNNEEELLRVWQKGDT
GYPFIDAGMRELWQTGFMHNRTRMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKNGEYIREWVPELKDMPNKYIHKPWEAPEHILQKANIQLGHTYPLPVVDHKAARERALCAYKSMKEFV

Sequences:

>Translated_476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSMGSASKWWLHHAIIDVKKQLEALGSTLIIRKGSTQEEIL
SLVEQLGITAVYWNICYDPDRLQFNQKMKMMLEHKGMICKEFNSHLLLEPWVIKKKDNTEYKVFTPFYNAFQKQVIHKPI
SKVQSIKGGNSLPVSLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTWKEFFSSKLASYSEGRDFPNQNAHSMLAPYLSF
GQISVKLIYHYLINKSTESQCSLFEKQVNSFIRQLIWREFSYYLLYHYPFTAYKPLNKSFEHFPWNNEEELLRVWQKGDT
GYPFIDAGMRELWQTGFMHNRTRMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKNGEYIREWVPELKDMPNKYIHKPWEAPEHILQKANIQLGHTYPLPVVDHKAARERALCAYKSMKEFV
>Mature_476_residues
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSMGSASKWWLHHAIIDVKKQLEALGSTLIIRKGSTQEEIL
SLVEQLGITAVYWNICYDPDRLQFNQKMKMMLEHKGMICKEFNSHLLLEPWVIKKKDNTEYKVFTPFYNAFQKQVIHKPI
SKVQSIKGGNSLPVSLSVSELHLLPTIPWTSHMESIWEPTEEGAYKTWKEFFSSKLASYSEGRDFPNQNAHSMLAPYLSF
GQISVKLIYHYLINKSTESQCSLFEKQVNSFIRQLIWREFSYYLLYHYPFTAYKPLNKSFEHFPWNNEEELLRVWQKGDT
GYPFIDAGMRELWQTGFMHNRTRMAVASFLVKHLLIPWQEGAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNP
ITQGEKFDKNGEYIREWVPELKDMPNKYIHKPWEAPEHILQKANIQLGHTYPLPVVDHKAARERALCAYKSMKEFV

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=514, Percent_Identity=28.988326848249, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4758072, Length=481, Percent_Identity=30.1455301455301, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI188536103, Length=475, Percent_Identity=28.2105263157895, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1786926, Length=480, Percent_Identity=34.5833333333333, Blast_Score=280, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6324962, Length=510, Percent_Identity=28.2352941176471, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI17137248, Length=502, Percent_Identity=28.2868525896414, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24585455, Length=502, Percent_Identity=28.2868525896414, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24648152, Length=511, Percent_Identity=25.440313111546, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55713; Mature: 55713

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSMGSASKWWLHHAIIDVKKQ
CCCCEEEEEECCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LEALGSTLIIRKGSTQEEILSLVEQLGITAVYWNICYDPDRLQFNQKMKMMLEHKGMICK
HHHCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHH
EFNSHLLLEPWVIKKKDNTEYKVFTPFYNAFQKQVIHKPISKVQSIKGGNSLPVSLSVSE
HCCCCEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
LHLLPTIPWTSHMESIWEPTEEGAYKTWKEFFSSKLASYSEGRDFPNQNAHSMLAPYLSF
EEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
GQISVKLIYHYLINKSTESQCSLFEKQVNSFIRQLIWREFSYYLLYHYPFTAYKPLNKSF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCCCC
EHFPWNNEEELLRVWQKGDTGYPFIDAGMRELWQTGFMHNRTRMAVASFLVKHLLIPWQE
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHH
GAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNPITQGEKFDKNGEYIREWVPE
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHH
LKDMPNKYIHKPWEAPEHILQKANIQLGHTYPLPVVDHKAARERALCAYKSMKEFV
HHCCCHHHHCCCCCCHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNKIIVMFQKDFRLYDNPALFEAAQSGEVVPVYVHDETFSMGSASKWWLHHAIIDVKKQ
CCCCEEEEEECCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LEALGSTLIIRKGSTQEEILSLVEQLGITAVYWNICYDPDRLQFNQKMKMMLEHKGMICK
HHHCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHH
EFNSHLLLEPWVIKKKDNTEYKVFTPFYNAFQKQVIHKPISKVQSIKGGNSLPVSLSVSE
HCCCCEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHH
LHLLPTIPWTSHMESIWEPTEEGAYKTWKEFFSSKLASYSEGRDFPNQNAHSMLAPYLSF
EEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
GQISVKLIYHYLINKSTESQCSLFEKQVNSFIRQLIWREFSYYLLYHYPFTAYKPLNKSF
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCCCC
EHFPWNNEEELLRVWQKGDTGYPFIDAGMRELWQTGFMHNRTRMAVASFLVKHLLIPWQE
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHH
GAKWFMDTLLDADIANNTMGWQWVAGSGADASPYFRIFNPITQGEKFDKNGEYIREWVPE
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCHHHCCCCCCCCHHHHHHHHHH
LKDMPNKYIHKPWEAPEHILQKANIQLGHTYPLPVVDHKAARERALCAYKSMKEFV
HHCCCHHHHCCCCCCHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]