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The map label for this gene is ywkE [H]

Identifier: 49476827

GI number: 49476827

Start: 5061380

End: 5062231

Strand: Reverse

Name: ywkE [H]

Synonym: BT9727_5012

Alternate gene names: 49476827

Gene position: 5062231-5061380 (Counterclockwise)

Preceding gene: 49480414

Following gene: 49481654

Centisome position: 96.65

GC content: 38.85

Gene sequence:

>852_bases
ATGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAGGAGAATGGGCGAGATGAAAATGCGGGAGAAATTGT
CCTTTGTCATGTATTAAAGACGAATAGAACAGGATTATTGATGAACATGCGTGAAGAAATAACTGCGGAACAAGAAACAA
GTTTTACAGAGTTTATCCACAAGCATGTAGAAGGTATTCCGATTCAATATATGATTGGTCATGAAATGTTTTATGGAAGA
TCGTTCATTGTGAATGAAGAAGTATTAATACCAAGACCGGAAACAGAAGAGCTTATAGTGGGAGTGTTAGAAAGAATCGA
GCGCCATTTTGGTGATGAGAAACTACATGTAGCAGATATCGGTACAGGTAGCGGAGCGATTTCGATTACACTTGCTTTAG
AGAATAAAGATCTTCATGTATATACGGTAGATATTGCACAGGAGTCAATTGAAGTTGCAAAAGAAAATGCGAAAACTTTG
GGCGCAGAAGTAACATTCTATCATGGTGATTTATTATCACCATTTTATGAAACGGGTCAAAAGTTAGATGTTGTTGTTTC
AAATCCTCCATATATACCGGAAGAAGATTGGCGTGGTCTTTCTCCTGTGGTGAAAGAGCATGAGCCAAAGAGAGCGCTTG
TTGGTGGTGAAGACGGATTAGATTTTTATCGCCGCTTTATGGAAGAGTTGCCGAATGTGTTGCAGAAGAAAGCAATTGTA
GCATTTGAAATTGGAGTAGGACAAGGTGAGGACGTGAAAGGGCTGCTACAACAAGCTTTTCCACATGCTCATGTTGAGGT
TGTTTTTGATATTAATGGAAAAGATCGTATGGTATTTGCAGAAATAGAGTAA

Upstream 100 bases:

>100_bases
CGATTCAAAAGCTAGATCAAATCTTACAAGGTAAGTTAGATGATTTCATCAATGCCTTAGTGATGGAAGATCAGGCTCAA
AGGATGGAGGCAGCTGAGTA

Downstream 100 bases:

>100_bases
GGATTACACCTTACTCTATTTTTTTGTGGAAAAATTCATATAGATTTTGAATTTAATAGTTTAGAAAGATGAAAGTCTGT
CCACACTGTTTGTAGAAGGG

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITAEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFIVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKDLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPNVLQKKAIV
AFEIGVGQGEDVKGLLQQAFPHAHVEVVFDINGKDRMVFAEIE

Sequences:

>Translated_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITAEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFIVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKDLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPNVLQKKAIV
AFEIGVGQGEDVKGLLQQAFPHAHVEVVFDINGKDRMVFAEIE
>Mature_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITAEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFIVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKDLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPNVLQKKAIV
AFEIGVGQGEDVKGLLQQAFPHAHVEVVFDINGKDRMVFAEIE

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=270, Percent_Identity=29.2592592592593, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787463, Length=281, Percent_Identity=30.6049822064057, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082085, Length=208, Percent_Identity=31.25, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=232, Percent_Identity=28.8793103448276, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582226, Length=243, Percent_Identity=26.7489711934156, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32008; Mature: 32008

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITAEQETSFTEFIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHHCCHHHHHHHHHHH
KHVEGIPIQYMIGHEMFYGRSFIVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADI
HHHCCCCEEEEECCHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GTGSGAISITLALENKDLHVYTVDIAQESIEVAKENAKTLGAEVTFYHGDLLSPFYETGQ
CCCCCEEEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPNVLQKKAIV
EEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEE
AFEIGVGQGEDVKGLLQQAFPHAHVEVVFDINGKDRMVFAEIE
EEEECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEC
>Mature Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITAEQETSFTEFIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHHCCHHHHHHHHHHH
KHVEGIPIQYMIGHEMFYGRSFIVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADI
HHHCCCCEEEEECCHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GTGSGAISITLALENKDLHVYTVDIAQESIEVAKENAKTLGAEVTFYHGDLLSPFYETGQ
CCCCCEEEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPNVLQKKAIV
EEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCEEE
AFEIGVGQGEDVKGLLQQAFPHAHVEVVFDINGKDRMVFAEIE
EEEECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]