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The map label for this gene is phaD [H]

Identifier: 49476294

GI number: 49476294

Start: 1895772

End: 1897394

Strand: Reverse

Name: phaD [H]

Synonym: BH16440

Alternate gene names: 49476294

Gene position: 1897394-1895772 (Counterclockwise)

Preceding gene: 49476295

Following gene: 49476293

Centisome position: 98.26

GC content: 38.94

Gene sequence:

>1623_bases
ATGAAAATTGGAGTAAATCATCTCCTTATTTTACCTATTCTTTTGCCATTAATTACTGGAGCACTTCTCCTTTTCTATGA
TGAACGCCGTTCAAAACTTAAATCATTAATCAGCATTTCTTCTACCGGATTATTAGTCATTGTTGCTGTTTTATTGATTA
TCCATGCTCTTAAAGTTGCGCCGGCTGCCGATGTTTATCGGCTTGGCAATTGGCCTTCTCCCTTTGGAATTGTGTTGGTC
CTTGATCGTTTAAGTTCTATGATGCTTTTACTTTCCAGCTTGTTGATCACAGCCGCGTTGGTTTTTGCACAGGCTCATTG
GTATAAAGCTGGTCCTCATTTTCAGTCACTGATGCAATTTTTCATGGTTGGAATAAACGGCGCTTTTTTGACAGGTGATT
TGTTCAATTTATTTGTGTTTTTTGAAGTTATGTTAACGGCTTCTTATGGGCTTGTGTTGCATGGCTCTGGGCAGTTACGT
GTACGTGCAGGATTGCATTATGTCGTCGTTAATCTTATTGCTTCGTTGTTTTTTTTAGTTGGTACGGCACTCATTTATGG
AATCGTTGGTACTCTTAATATGGCCGATTTGGCTGTAAAAATGAAACATATAACCTCTGCGGATATTGTTTTATTTGAAA
TAGGCGCGGCGCTTTTGGGGATTGCTTTTTTACTCAAAGTGGGTATGTGGCCACTTAATTTTTGGTTGATGCCGACTTAT
AGTGCCGCAGCAGCACCCGTTGGAGCTTCTTTTGCACTTTTAAGCAAGGTGGGTATTTATATTGTTTTACGTCTGACATT
GCTATGGTTTGGTCCTGAAAGTGAATATTTTAGTCATTTTGGTAAAATGGTTTTATTTTATGGTGGACTTGCCACTATGG
CTTTTGGTTTTATTGGGGTCTTGGCGAGCCAAGTTTTGGTGCGTTTGGTGGCTTATAGCATTCTTGTCTCTTCTGGTACT
TTGTTGGCAACCATTGGGATCGGCAATATAGCACTCACAGCAGGTGCACTCTTTTATATTGTTTCTTCAACTTTAGCCTT
TGGTGCTTTTTTTCTTTTGGTGGAGTTGGTTGAACGCTGTCAAGATGTGGCTGCCAATGTTTTAACTGTTACAATGGAAG
TCTATGGTGACGATGAAGAAGAGGAAGAGGATGAAGTTGGTACTTATTTGCCGGTAACTTTGGCAATTCTTGGAGTCTGT
TTTGGTATCTGTGCCATTTTGATTATTGGTCTACCACCTTTTTCTGGTTTTATTGCTAAATTTATGATGTTTATGGCAAT
CTTAAACCACAATAAAGAAGATTTTGCTGCTTCTTTACCGGTTCATTATGATTGGCTTTTTGTGATTTTTGTTACCATTT
CTGGTTTTGCAGCTTTAATTGCTCTGACACGAACGGGTATTCGAACTTTTTGGGTGTCTCATGAAGGTACAGTTCCGCGT
GTGCAGGTGATCGAATTTGTACCTATTGCTGTTCTCCTTACTTTGTGTGTTCTTATAACAATTATAGCCGGTCCTGTTAG
TCATTATATGTCTGAAACTGCTAAGACTTTGTATGAACCCCGGAACTATATTGGAAGTGTTTTAGATGATTTTTCTCTCA
AAAATAGGGAGATGAATCAATGA

Upstream 100 bases:

>100_bases
TTGTTATTGGTTTTGCAACGACAGCTTTATTTTTGGTTATCCTTCTGGTTTCACGCGGTTTAACGGGTTCAGATCACGTT
GATGGACGTGAGGTGCAGTG

Downstream 100 bases:

>100_bases
AATATTTTTGTCTATTTCCTTTTTTCAGTGCAGTCATTGTTTTTATGTGGCTGACTTTAAATGGTTTTAATTTAGGTCAA
TTGCTTTTAGGTATTATAAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKIGVNHLLILPILLPLITGALLLFYDERRSKLKSLISISSTGLLVIVAVLLIIHALKVAPAADVYRLGNWPSPFGIVLV
LDRLSSMMLLLSSLLITAALVFAQAHWYKAGPHFQSLMQFFMVGINGAFLTGDLFNLFVFFEVMLTASYGLVLHGSGQLR
VRAGLHYVVVNLIASLFFLVGTALIYGIVGTLNMADLAVKMKHITSADIVLFEIGAALLGIAFLLKVGMWPLNFWLMPTY
SAAAAPVGASFALLSKVGIYIVLRLTLLWFGPESEYFSHFGKMVLFYGGLATMAFGFIGVLASQVLVRLVAYSILVSSGT
LLATIGIGNIALTAGALFYIVSSTLAFGAFFLLVELVERCQDVAANVLTVTMEVYGDDEEEEEDEVGTYLPVTLAILGVC
FGICAILIIGLPPFSGFIAKFMMFMAILNHNKEDFAASLPVHYDWLFVIFVTISGFAALIALTRTGIRTFWVSHEGTVPR
VQVIEFVPIAVLLTLCVLITIIAGPVSHYMSETAKTLYEPRNYIGSVLDDFSLKNREMNQ

Sequences:

>Translated_540_residues
MKIGVNHLLILPILLPLITGALLLFYDERRSKLKSLISISSTGLLVIVAVLLIIHALKVAPAADVYRLGNWPSPFGIVLV
LDRLSSMMLLLSSLLITAALVFAQAHWYKAGPHFQSLMQFFMVGINGAFLTGDLFNLFVFFEVMLTASYGLVLHGSGQLR
VRAGLHYVVVNLIASLFFLVGTALIYGIVGTLNMADLAVKMKHITSADIVLFEIGAALLGIAFLLKVGMWPLNFWLMPTY
SAAAAPVGASFALLSKVGIYIVLRLTLLWFGPESEYFSHFGKMVLFYGGLATMAFGFIGVLASQVLVRLVAYSILVSSGT
LLATIGIGNIALTAGALFYIVSSTLAFGAFFLLVELVERCQDVAANVLTVTMEVYGDDEEEEEDEVGTYLPVTLAILGVC
FGICAILIIGLPPFSGFIAKFMMFMAILNHNKEDFAASLPVHYDWLFVIFVTISGFAALIALTRTGIRTFWVSHEGTVPR
VQVIEFVPIAVLLTLCVLITIIAGPVSHYMSETAKTLYEPRNYIGSVLDDFSLKNREMNQ
>Mature_540_residues
MKIGVNHLLILPILLPLITGALLLFYDERRSKLKSLISISSTGLLVIVAVLLIIHALKVAPAADVYRLGNWPSPFGIVLV
LDRLSSMMLLLSSLLITAALVFAQAHWYKAGPHFQSLMQFFMVGINGAFLTGDLFNLFVFFEVMLTASYGLVLHGSGQLR
VRAGLHYVVVNLIASLFFLVGTALIYGIVGTLNMADLAVKMKHITSADIVLFEIGAALLGIAFLLKVGMWPLNFWLMPTY
SAAAAPVGASFALLSKVGIYIVLRLTLLWFGPESEYFSHFGKMVLFYGGLATMAFGFIGVLASQVLVRLVAYSILVSSGT
LLATIGIGNIALTAGALFYIVSSTLAFGAFFLLVELVERCQDVAANVLTVTMEVYGDDEEEEEDEVGTYLPVTLAILGVC
FGICAILIIGLPPFSGFIAKFMMFMAILNHNKEDFAASLPVHYDWLFVIFVTISGFAALIALTRTGIRTFWVSHEGTVPR
VQVIEFVPIAVLLTLCVLITIIAGPVSHYMSETAKTLYEPRNYIGSVLDDFSLKNREMNQ

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788829, Length=439, Percent_Identity=23.4624145785877, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788613, Length=439, Percent_Identity=25.5125284738041, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 58870; Mature: 58870

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVNHLLILPILLPLITGALLLFYDERRSKLKSLISISSTGLLVIVAVLLIIHALKVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PAADVYRLGNWPSPFGIVLVLDRLSSMMLLLSSLLITAALVFAQAHWYKAGPHFQSLMQF
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FMVGINGAFLTGDLFNLFVFFEVMLTASYGLVLHGSGQLRVRAGLHYVVVNLIASLFFLV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
GTALIYGIVGTLNMADLAVKMKHITSADIVLFEIGAALLGIAFLLKVGMWPLNFWLMPTY
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCC
SAAAAPVGASFALLSKVGIYIVLRLTLLWFGPESEYFSHFGKMVLFYGGLATMAFGFIGV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
LASQVLVRLVAYSILVSSGTLLATIGIGNIALTAGALFYIVSSTLAFGAFFLLVELVERC
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDVAANVLTVTMEVYGDDEEEEEDEVGTYLPVTLAILGVCFGICAILIIGLPPFSGFIAK
HHHHHHHHHEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FMMFMAILNHNKEDFAASLPVHYDWLFVIFVTISGFAALIALTRTGIRTFWVSHEGTVPR
HHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCH
VQVIEFVPIAVLLTLCVLITIIAGPVSHYMSETAKTLYEPRNYIGSVLDDFSLKNREMNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIGVNHLLILPILLPLITGALLLFYDERRSKLKSLISISSTGLLVIVAVLLIIHALKVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PAADVYRLGNWPSPFGIVLVLDRLSSMMLLLSSLLITAALVFAQAHWYKAGPHFQSLMQF
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FMVGINGAFLTGDLFNLFVFFEVMLTASYGLVLHGSGQLRVRAGLHYVVVNLIASLFFLV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
GTALIYGIVGTLNMADLAVKMKHITSADIVLFEIGAALLGIAFLLKVGMWPLNFWLMPTY
HHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCC
SAAAAPVGASFALLSKVGIYIVLRLTLLWFGPESEYFSHFGKMVLFYGGLATMAFGFIGV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
LASQVLVRLVAYSILVSSGTLLATIGIGNIALTAGALFYIVSSTLAFGAFFLLVELVERC
HHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDVAANVLTVTMEVYGDDEEEEEDEVGTYLPVTLAILGVCFGICAILIIGLPPFSGFIAK
HHHHHHHHHEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FMMFMAILNHNKEDFAASLPVHYDWLFVIFVTISGFAALIALTRTGIRTFWVSHEGTVPR
HHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCH
VQVIEFVPIAVLLTLCVLITIIAGPVSHYMSETAKTLYEPRNYIGSVLDDFSLKNREMNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]