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The map label for this gene is purH

Identifier: 49476249

GI number: 49476249

Start: 1840031

End: 1841647

Strand: Reverse

Name: purH

Synonym: BH15970

Alternate gene names: 49476249

Gene position: 1841647-1840031 (Counterclockwise)

Preceding gene: 49476250

Following gene: 49476248

Centisome position: 95.37

GC content: 42.86

Gene sequence:

>1617_bases
ATGGGTGTTGTTGCAAAAAATTTTCCCATACCTGATCTTCATCGGGTCCGTCGTGTTCTGCTTTCTGTGTCTGATAAAAC
GGGTATAGTTGCGTTTGCGCAAGCGCTTCAGACTTATAATGTTGAATTGATTTCGACAGGAGGAACAGCTAAAGTCTTAA
TGGCTGCTGGTTTACCGGTAAGAGATGTTGCTGAAGTTACAGGATTTCCCGAAATAATGGATGGACGCGTCAAAACACTC
CATCCTTTGATCCATGGTGCTCTCTTGGGAGTAAGAGAAGATCCTAGCCACGCAGTGGCTATGGAGCAGTATGGCATCCA
TGGAATCGATCTTCTGGTGGTCAATCTTTATCCTTTTGAAGAAACTGTTCAATCAGGCGCTGATGGACAAACAATTCTTG
AAAATATTGATATTGGTGGACCGGCAATGATTCGCGCGGCGGCAAAGAACCATGTGTATACTGGTGTTATCACAGCGGTG
AGTGATTATGATGCAGTTCTTTCTGAATTAAAGCAGCATGATGGGTGTTTAAGTTTTTCTATGCGACAACAGTTAGCAAT
GCGTGCTTATGCGCACACGGCTGCTTATGATACGGCGATAGCGGCGTGGTTTGCAAAAAATTTAAAAATTGAAACACCCT
CTTGGCAGAGTTTTTCTGGTCATCTTAAAAATGTCATGCGTTATGGGGAAAACCCGCATCAAAAGGCAGCATTTTATCGT
AATGGTGACAAACGTTTTGGTGTTGCAACAGCCAAACTTTTACAGGGTAAAGCACTCTCTTATAACAATATGAATGATAC
AGATGCAGCATTTGAACTTGTGGCAGAATTTGATCCACAAAACACTGCTGCTGTTGCTCTTATTAAACATGCTAACCCTT
GTGGGGTTGCTGAAGGGCAGACTTTAAAGGAAGCTTATTTGAAGGCTCTTCTATGTGATAATGTATCGGCATTTGGTGGA
ATTATTGCTTTAAATCAACCCCTTGATGCAGAATGTGCAGAAGAGGTTATCAAAATTTTCACGGAAGTGATTATTGCTCC
TGATGCAACAATGGAGGCACGTGAAATTATTTCAGGTAAAAAAAATCTTCGTTTGCTGCTAACAGGGGGTGTTCCTGATC
CACGGTGTGGAGGATTTATAGCCAAAACCCTTGCAGGCGGGATTTTGGTGCAATCGCGTGATAATGTGGTGGTTGATGAT
CTCAAACTGCAAGTGGTGACAAAGCGCGCACCAAGTCAAGAGGAAATGCGTGATCTTCAATTTGCTTTTCGTGTGGTAAA
ACATGTTAAATCAAATGCTATTGTTTATGCAAAAAATAGCGCAACAGTTGGAATTGGTGCTGGACAGATGAGTCGGGTTG
ATTCAGCAAAAATTGCTGCACGTAAAGCTGAAGAAAGTGCAAAAAGAGCAGGTTTAACGGAATCTCTCACCAAAGGGTCC
GTTGTTGCATCAGATGCCTTTTTTCCTTTTGCAGATGGTTTGTTGGCAGTTGCTGAAGCAGGAGCTACAGCAGTTATTCA
ACCGGGTGGATCAATGCGTGATGAAGAAGTGATTGCCGCGGCCGATGCACAGGGATTAGCTATGGTTTTTACAGGTGTAA
GGCATTTTCGTCATTAG

Upstream 100 bases:

>100_bases
GTATTTTGCTAAAAGCATGTGCTATAATATATAAATTGGTTTTATGCATATAAGCTAGTTTTTCTTGAATTTTAACCATT
TCATTTTTAGGATTTCTGTT

Downstream 100 bases:

>100_bases
TGCTTTTATTCTTGGGATGTTGATCTTTTCAACTAAGATAAATTTTACGGAAAATGAGTGTGATCACTTTTGCATAAGCG
TTTCGCATTTTCCATGTTGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MGVVAKNFPIPDLHRVRRVLLSVSDKTGIVAFAQALQTYNVELISTGGTAKVLMAAGLPVRDVAEVTGFPEIMDGRVKTL
HPLIHGALLGVREDPSHAVAMEQYGIHGIDLLVVNLYPFEETVQSGADGQTILENIDIGGPAMIRAAAKNHVYTGVITAV
SDYDAVLSELKQHDGCLSFSMRQQLAMRAYAHTAAYDTAIAAWFAKNLKIETPSWQSFSGHLKNVMRYGENPHQKAAFYR
NGDKRFGVATAKLLQGKALSYNNMNDTDAAFELVAEFDPQNTAAVALIKHANPCGVAEGQTLKEAYLKALLCDNVSAFGG
IIALNQPLDAECAEEVIKIFTEVIIAPDATMEAREIISGKKNLRLLLTGGVPDPRCGGFIAKTLAGGILVQSRDNVVVDD
LKLQVVTKRAPSQEEMRDLQFAFRVVKHVKSNAIVYAKNSATVGIGAGQMSRVDSAKIAARKAEESAKRAGLTESLTKGS
VVASDAFFPFADGLLAVAEAGATAVIQPGGSMRDEEVIAAADAQGLAMVFTGVRHFRH

Sequences:

>Translated_538_residues
MGVVAKNFPIPDLHRVRRVLLSVSDKTGIVAFAQALQTYNVELISTGGTAKVLMAAGLPVRDVAEVTGFPEIMDGRVKTL
HPLIHGALLGVREDPSHAVAMEQYGIHGIDLLVVNLYPFEETVQSGADGQTILENIDIGGPAMIRAAAKNHVYTGVITAV
SDYDAVLSELKQHDGCLSFSMRQQLAMRAYAHTAAYDTAIAAWFAKNLKIETPSWQSFSGHLKNVMRYGENPHQKAAFYR
NGDKRFGVATAKLLQGKALSYNNMNDTDAAFELVAEFDPQNTAAVALIKHANPCGVAEGQTLKEAYLKALLCDNVSAFGG
IIALNQPLDAECAEEVIKIFTEVIIAPDATMEAREIISGKKNLRLLLTGGVPDPRCGGFIAKTLAGGILVQSRDNVVVDD
LKLQVVTKRAPSQEEMRDLQFAFRVVKHVKSNAIVYAKNSATVGIGAGQMSRVDSAKIAARKAEESAKRAGLTESLTKGS
VVASDAFFPFADGLLAVAEAGATAVIQPGGSMRDEEVIAAADAQGLAMVFTGVRHFRH
>Mature_537_residues
GVVAKNFPIPDLHRVRRVLLSVSDKTGIVAFAQALQTYNVELISTGGTAKVLMAAGLPVRDVAEVTGFPEIMDGRVKTLH
PLIHGALLGVREDPSHAVAMEQYGIHGIDLLVVNLYPFEETVQSGADGQTILENIDIGGPAMIRAAAKNHVYTGVITAVS
DYDAVLSELKQHDGCLSFSMRQQLAMRAYAHTAAYDTAIAAWFAKNLKIETPSWQSFSGHLKNVMRYGENPHQKAAFYRN
GDKRFGVATAKLLQGKALSYNNMNDTDAAFELVAEFDPQNTAAVALIKHANPCGVAEGQTLKEAYLKALLCDNVSAFGGI
IALNQPLDAECAEEVIKIFTEVIIAPDATMEAREIISGKKNLRLLLTGGVPDPRCGGFIAKTLAGGILVQSRDNVVVDDL
KLQVVTKRAPSQEEMRDLQFAFRVVKHVKSNAIVYAKNSATVGIGAGQMSRVDSAKIAARKAEESAKRAGLTESLTKGSV
VASDAFFPFADGLLAVAEAGATAVIQPGGSMRDEEVIAAADAQGLAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=600, Percent_Identity=34.5, Blast_Score=273, Evalue=4e-73,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=53.8175046554935, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71985564, Length=604, Percent_Identity=30.9602649006623, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=26.5573770491803, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=601, Percent_Identity=31.7803660565724, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=34.2494714587738, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24649832, Length=599, Percent_Identity=34.2237061769616, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_BARHE (Q6G5S5)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034290.1
- HSSP:   P31939
- ProteinModelPortal:   Q6G5S5
- GeneID:   2864772
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH15970
- NMPDR:   fig|283166.1.peg.1418
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q6G5S5
- ProtClustDB:   PRK00881
- BioCyc:   BHEN283166:BH15970-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57598; Mature: 57466

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVAKNFPIPDLHRVRRVLLSVSDKTGIVAFAQALQTYNVELISTGGTAKVLMAAGLPV
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHEECCCCH
RDVAEVTGFPEIMDGRVKTLHPLIHGALLGVREDPSHAVAMEQYGIHGIDLLVVNLYPFE
HHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEECCCH
ETVQSGADGQTILENIDIGGPAMIRAAAKNHVYTGVITAVSDYDAVLSELKQHDGCLSFS
HHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCEEEEHEEHHHHHHHHHHHHHHCCCCCCHH
MRQQLAMRAYAHTAAYDTAIAAWFAKNLKIETPSWQSFSGHLKNVMRYGENPHQKAAFYR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHEE
NGDKRFGVATAKLLQGKALSYNNMNDTDAAFELVAEFDPQNTAAVALIKHANPCGVAEGQ
CCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
TLKEAYLKALLCDNVSAFGGIIALNQPLDAECAEEVIKIFTEVIIAPDATMEAREIISGK
HHHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
KNLRLLLTGGVPDPRCGGFIAKTLAGGILVQSRDNVVVDDLKLQVVTKRAPSQEEMRDLQ
CCEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCEEEECEEEEEEECCCCCHHHHHHHH
FAFRVVKHVKSNAIVYAKNSATVGIGAGQMSRVDSAKIAARKAEESAKRAGLTESLTKGS
HHHHHHHHHCCCEEEEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCC
VVASDAFFPFADGLLAVAEAGATAVIQPGGSMRDEEVIAAADAQGLAMVFTGVRHFRH
EEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GVVAKNFPIPDLHRVRRVLLSVSDKTGIVAFAQALQTYNVELISTGGTAKVLMAAGLPV
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHEECCCCH
RDVAEVTGFPEIMDGRVKTLHPLIHGALLGVREDPSHAVAMEQYGIHGIDLLVVNLYPFE
HHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEECCCH
ETVQSGADGQTILENIDIGGPAMIRAAAKNHVYTGVITAVSDYDAVLSELKQHDGCLSFS
HHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCEEEEHEEHHHHHHHHHHHHHHCCCCCCHH
MRQQLAMRAYAHTAAYDTAIAAWFAKNLKIETPSWQSFSGHLKNVMRYGENPHQKAAFYR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHEE
NGDKRFGVATAKLLQGKALSYNNMNDTDAAFELVAEFDPQNTAAVALIKHANPCGVAEGQ
CCCCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
TLKEAYLKALLCDNVSAFGGIIALNQPLDAECAEEVIKIFTEVIIAPDATMEAREIISGK
HHHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
KNLRLLLTGGVPDPRCGGFIAKTLAGGILVQSRDNVVVDDLKLQVVTKRAPSQEEMRDLQ
CCEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCEEEECEEEEEEECCCCCHHHHHHHH
FAFRVVKHVKSNAIVYAKNSATVGIGAGQMSRVDSAKIAARKAEESAKRAGLTESLTKGS
HHHHHHHHHCCCEEEEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCC
VVASDAFFPFADGLLAVAEAGATAVIQPGGSMRDEEVIAAADAQGLAMVFTGVRHFRH
EEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA