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The map label for this gene is emrA [H]

Identifier: 49476184

GI number: 49476184

Start: 1759709

End: 1760713

Strand: Reverse

Name: emrA [H]

Synonym: BH15290

Alternate gene names: 49476184

Gene position: 1760713-1759709 (Counterclockwise)

Preceding gene: 49476185

Following gene: 49476182

Centisome position: 91.18

GC content: 41.49

Gene sequence:

>1005_bases
ATGATAAAAGCATTACGATCAAGAGCGACGATTATTGCATTCTTCTCAGGGATTACAGGTTGTTTACTGATTTTATGGGC
TTGGAAGCTTCCTCCTTTTGTCAGTGCAATTCAAATAACCGATAATGCTTCCGTTAAAGGCAATATGACGCTGGTGAGTT
CACAAATCTCTGGTGTAATTGCACGGATTTATGTGCAAGATTATCAAAGAGTTGAACAGGGAATGCTGCTCTTTGAACTC
GATGATTCCCTCTTTCGTCAACAACTTGCACGGGCACAGGCTGTTTTGGATTCAAAAAATGCAAAACTTGCAAGTGTTGT
TTTGCAGGCGCAGCTTTTGCAAGGAGAAATTAATACAGCAGAAGCCGAATTGACCCGTTCACGAGCATTCTCCAATGTTA
TTCCTGAAAACAAGAAGTTAGGTTTTGGGGATGGGGCAAGTTCTGTTTCTCGTCCTCTTTCGCAATTATTGGCAGCACTT
GAAACAAAACGCCAGTTGCAAAAACAATTGGATCTTGAACGACAAAGTTTGCAGTCAGAGGTTGCTGGAGCAAAGGTTGG
CGTTGAATTAGCGAAGCTGAATTTGAAGCACACGAAGATTTTATCTCCTAAAACCGGACATGTTGGATTGGTTGGTGCAC
GGGTAGGACAATATGTTTTGCCTGGTACGCAATTGGTGTCACTCATTTCTGATGATATTTGGATTATTGCCAATTATAAA
GAAACGCAACTTTCTCAGATGCGTGTTGGGCAACCTGTTGTCTTTTCGGTTGATGCATTGAACAATCAGAAATTAACAGG
ACGTGTGGTTCGTTTCGCACCTGCAACAGGTTCGGAATTTTCATTGTTAAAAACAGATACTGCAATTGGTAATTTCATCA
AGATTGCGCAACGTATTTCAGTGCGTATTTCTTTAGATCCTGGACAAGAAGGGGTGGAAAAGCTTATTCCTGGGATGTCT
GTTGTTACCTATGTCGATACTTCACAACGCGGTTCTGGAGAATAA

Upstream 100 bases:

>100_bases
CGGTTGTATTTGCTATTTTTCTTGCCAAAATATTTTTAAAAGCATGCTTTTTGAATGCTTTTAAAAAGCAGAGCAATCTT
ATACAAGAGGAGTGCAAAAA

Downstream 100 bases:

>100_bases
AATTTGCTTTTTTGTGTTTGATTTTTACCCCTTTTATTTATAAAAAATGGGCTTTTATTTTGGAGGATTTCTTTTGGTTT
GTTCTAGTGCAGAGGGTTAT

Product: transport protein transmembrane

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIKALRSRATIIAFFSGITGCLLILWAWKLPPFVSAIQITDNASVKGNMTLVSSQISGVIARIYVQDYQRVEQGMLLFEL
DDSLFRQQLARAQAVLDSKNAKLASVVLQAQLLQGEINTAEAELTRSRAFSNVIPENKKLGFGDGASSVSRPLSQLLAAL
ETKRQLQKQLDLERQSLQSEVAGAKVGVELAKLNLKHTKILSPKTGHVGLVGARVGQYVLPGTQLVSLISDDIWIIANYK
ETQLSQMRVGQPVVFSVDALNNQKLTGRVVRFAPATGSEFSLLKTDTAIGNFIKIAQRISVRISLDPGQEGVEKLIPGMS
VVTYVDTSQRGSGE

Sequences:

>Translated_334_residues
MIKALRSRATIIAFFSGITGCLLILWAWKLPPFVSAIQITDNASVKGNMTLVSSQISGVIARIYVQDYQRVEQGMLLFEL
DDSLFRQQLARAQAVLDSKNAKLASVVLQAQLLQGEINTAEAELTRSRAFSNVIPENKKLGFGDGASSVSRPLSQLLAAL
ETKRQLQKQLDLERQSLQSEVAGAKVGVELAKLNLKHTKILSPKTGHVGLVGARVGQYVLPGTQLVSLISDDIWIIANYK
ETQLSQMRVGQPVVFSVDALNNQKLTGRVVRFAPATGSEFSLLKTDTAIGNFIKIAQRISVRISLDPGQEGVEKLIPGMS
VVTYVDTSQRGSGE
>Mature_334_residues
MIKALRSRATIIAFFSGITGCLLILWAWKLPPFVSAIQITDNASVKGNMTLVSSQISGVIARIYVQDYQRVEQGMLLFEL
DDSLFRQQLARAQAVLDSKNAKLASVVLQAQLLQGEINTAEAELTRSRAFSNVIPENKKLGFGDGASSVSRPLSQLLAAL
ETKRQLQKQLDLERQSLQSEVAGAKVGVELAKLNLKHTKILSPKTGHVGLVGARVGQYVLPGTQLVSLISDDIWIIANYK
ETQLSQMRVGQPVVFSVDALNNQKLTGRVVRFAPATGSEFSLLKTDTAIGNFIKIAQRISVRISLDPGQEGVEKLIPGMS
VVTYVDTSQRGSGE

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=307, Percent_Identity=31.9218241042345, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788711, Length=334, Percent_Identity=30.5389221556886, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081951, Length=310, Percent_Identity=24.5161290322581, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790519, Length=294, Percent_Identity=27.2108843537415, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790024, Length=242, Percent_Identity=24.3801652892562, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36318; Mature: 36318

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKALRSRATIIAFFSGITGCLLILWAWKLPPFVSAIQITDNASVKGNMTLVSSQISGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCEEEHHHHHHHH
ARIYVQDYQRVEQGMLLFELDDSLFRQQLARAQAVLDSKNAKLASVVLQAQLLQGEINTA
HHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHH
EAELTRSRAFSNVIPENKKLGFGDGASSVSRPLSQLLAALETKRQLQKQLDLERQSLQSE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGAKVGVELAKLNLKHTKILSPKTGHVGLVGARVGQYVLPGTQLVSLISDDIWIIANYK
HCCHHHHHHHHHCCCCHHEECCCCCCCEEEEHHHHCCEECCHHHHHHHHCCCEEEEECCC
ETQLSQMRVGQPVVFSVDALNNQKLTGRVVRFAPATGSEFSLLKTDTAIGNFIKIAQRIS
HHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHHHHHEE
VRISLDPGQEGVEKLIPGMSVVTYVDTSQRGSGE
EEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MIKALRSRATIIAFFSGITGCLLILWAWKLPPFVSAIQITDNASVKGNMTLVSSQISGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCEEEHHHHHHHH
ARIYVQDYQRVEQGMLLFELDDSLFRQQLARAQAVLDSKNAKLASVVLQAQLLQGEINTA
HHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHH
EAELTRSRAFSNVIPENKKLGFGDGASSVSRPLSQLLAALETKRQLQKQLDLERQSLQSE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGAKVGVELAKLNLKHTKILSPKTGHVGLVGARVGQYVLPGTQLVSLISDDIWIIANYK
HCCHHHHHHHHHCCCCHHEECCCCCCCEEEEHHHHCCEECCHHHHHHHHCCCEEEEECCC
ETQLSQMRVGQPVVFSVDALNNQKLTGRVVRFAPATGSEFSLLKTDTAIGNFIKIAQRIS
HHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHHHHHEE
VRISLDPGQEGVEKLIPGMSVVTYVDTSQRGSGE
EEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]