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The map label for this gene is ispG

Identifier: 49476182

GI number: 49476182

Start: 1757483

End: 1758724

Strand: Reverse

Name: ispG

Synonym: BH15270

Alternate gene names: 49476182

Gene position: 1758724-1757483 (Counterclockwise)

Preceding gene: 49476184

Following gene: 49476180

Centisome position: 91.08

GC content: 44.36

Gene sequence:

>1242_bases
ATGAACACAGTGTATTCTTTATCTAAACCTCTTGAACGTCGCCAATCTGTTGCTGTTACGGTTGGTGATGTGCTGGTTGG
GGGGAGGAGTCCAATTGTTGTTCAATCAATGACTAATACGGATACTGCTGATGTTGATGCAACAGTTGCACAAGTTGCAG
CTCTTTGGCAGGCCGGTTCGCAATTGGTTCGCATCACTGTTGATCGTTATGAAGCTGCTGCTGCTGTGCCCAAAATACGG
GAGCGATTGGAACGTTTAGGATTCGTTATACCATTGGTGGGGGATTTTCATTATATTGGACACAAGCTTTTATCAGAACA
TCCTGCATGTGCTGAGGCTCTGGCAAAATATCGAATCAATCCTGGGAATGTTGGCTTTGGTGCAAAAAAGGATCGTCAGT
TTGCCGAAATTATCGAGATTGCCTGTCGCTATCGTAAACCTATTCGCATTGGGGTGAATTGGGGGTCGCTTGATCATGTT
TTGTTAACACGGCTTATGGATGAAAATGCTCAGCAGGAAAACCCTTTGTCTGGTGGTGAAATTATGCGGGAAGCTGTTGT
TCAGTCGGCTCTTCATTCGGCTCTTTGGGCTGAAGAGATGGGGTTGAAACGTGATCAGATTATTCTTTCTGCGAAAGTGA
GTGATGTACAAGATCTCATCGCTGTCTACAGTACTTTGGCGGAACGTTGTGATTATGCGCTCCATTTGGGATTAACAGAA
GCAGGAATGGGAACAAAGGGCATTGTTGCTTCTTCCGTGGCTTTGGGAATTTTGTTACAGAAAGGAATTGGTGATACGAT
TCGTATTTCTTTAACACCTGAACCTGGTGGAGATCGGACACGGGAAGTAAAGGTTGCTCAGGAGCTTTTGCAGGTGATGG
GGTTTCGGCAATTTTTTCCCGTTGTTACGGCTTGCCCTGGATGTGGTCGTACAACCTCGACAGTGTTTCAGCAATTGGCA
CAAAAAATTGAAACAGATTTACACAAAAATATGTCTGTATGGCGTGAAAAATATCCTGGTGTTGAAAGTTTAAAGGTTGC
GGTGATGGGGTGTATTGTCAATGGACCGGGAGAATCAAAACATGCTGATATTGGCATTTCATTGCCTGGAACAGGAGAAA
GTCCAGCAGCACCTGTTTTTATTGAGGGGCAAAAGGTGAAGACTTTGCGGGGAACCCATATCGCTGAAGAATTTGAACTT
CTCTTAAGTGATTATATTCATACACGTTTTGGGCAAGGTTGA

Upstream 100 bases:

>100_bases
TTTCATTCCTTTTTTCAAGTTGTACTCTTTTAATTGTGCTCTGGTAAGCGTTGCCGTAAATGATGATCGATAACGCAGGT
TTTGAAAATTTGAGGGCTTG

Downstream 100 bases:

>100_bases
AAAAGATTCTTGCAGCTCACTGTCTTTAAAGCATTTCTCTTTGATAACACTGTTTCTAAATAGATTCATCTGCAAATTAA
ATGTAAGATTATCTCCAGCA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNTVYSLSKPLERRQSVAVTVGDVLVGGRSPIVVQSMTNTDTADVDATVAQVAALWQAGSQLVRITVDRYEAAAAVPKIR
ERLERLGFVIPLVGDFHYIGHKLLSEHPACAEALAKYRINPGNVGFGAKKDRQFAEIIEIACRYRKPIRIGVNWGSLDHV
LLTRLMDENAQQENPLSGGEIMREAVVQSALHSALWAEEMGLKRDQIILSAKVSDVQDLIAVYSTLAERCDYALHLGLTE
AGMGTKGIVASSVALGILLQKGIGDTIRISLTPEPGGDRTREVKVAQELLQVMGFRQFFPVVTACPGCGRTTSTVFQQLA
QKIETDLHKNMSVWREKYPGVESLKVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIEGQKVKTLRGTHIAEEFEL
LLSDYIHTRFGQG

Sequences:

>Translated_413_residues
MNTVYSLSKPLERRQSVAVTVGDVLVGGRSPIVVQSMTNTDTADVDATVAQVAALWQAGSQLVRITVDRYEAAAAVPKIR
ERLERLGFVIPLVGDFHYIGHKLLSEHPACAEALAKYRINPGNVGFGAKKDRQFAEIIEIACRYRKPIRIGVNWGSLDHV
LLTRLMDENAQQENPLSGGEIMREAVVQSALHSALWAEEMGLKRDQIILSAKVSDVQDLIAVYSTLAERCDYALHLGLTE
AGMGTKGIVASSVALGILLQKGIGDTIRISLTPEPGGDRTREVKVAQELLQVMGFRQFFPVVTACPGCGRTTSTVFQQLA
QKIETDLHKNMSVWREKYPGVESLKVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIEGQKVKTLRGTHIAEEFEL
LLSDYIHTRFGQG
>Mature_413_residues
MNTVYSLSKPLERRQSVAVTVGDVLVGGRSPIVVQSMTNTDTADVDATVAQVAALWQAGSQLVRITVDRYEAAAAVPKIR
ERLERLGFVIPLVGDFHYIGHKLLSEHPACAEALAKYRINPGNVGFGAKKDRQFAEIIEIACRYRKPIRIGVNWGSLDHV
LLTRLMDENAQQENPLSGGEIMREAVVQSALHSALWAEEMGLKRDQIILSAKVSDVQDLIAVYSTLAERCDYALHLGLTE
AGMGTKGIVASSVALGILLQKGIGDTIRISLTPEPGGDRTREVKVAQELLQVMGFRQFFPVVTACPGCGRTTSTVFQQLA
QKIETDLHKNMSVWREKYPGVESLKVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIEGQKVKTLRGTHIAEEFEL
LLSDYIHTRFGQG

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=396, Percent_Identity=33.0808080808081, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BARHE (Q6G1X4)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034223.1
- ProteinModelPortal:   Q6G1X4
- GeneID:   2864733
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH15270
- NMPDR:   fig|283166.1.peg.1351
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q6G1X4
- ProtClustDB:   PRK00366
- BioCyc:   BHEN283166:BH15270-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44908; Mature: 44908

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVYSLSKPLERRQSVAVTVGDVLVGGRSPIVVQSMTNTDTADVDATVAQVAALWQAGS
CCCCHHHHHHHHHHHHEEEEEHHEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC
QLVRITVDRYEAAAAVPKIRERLERLGFVIPLVGDFHYIGHKLLSEHPACAEALAKYRIN
CEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PGNVGFGAKKDRQFAEIIEIACRYRKPIRIGVNWGSLDHVLLTRLMDENAQQENPLSGGE
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHH
IMREAVVQSALHSALWAEEMGLKRDQIILSAKVSDVQDLIAVYSTLAERCDYALHLGLTE
HHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
AGMGTKGIVASSVALGILLQKGIGDTIRISLTPEPGGDRTREVKVAQELLQVMGFRQFFP
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VVTACPGCGRTTSTVFQQLAQKIETDLHKNMSVWREKYPGVESLKVAVMGCIVNGPGESK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCC
HADIGISLPGTGESPAAPVFIEGQKVKTLRGTHIAEEFELLLSDYIHTRFGQG
CCEEEEECCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTVYSLSKPLERRQSVAVTVGDVLVGGRSPIVVQSMTNTDTADVDATVAQVAALWQAGS
CCCCHHHHHHHHHHHHEEEEEHHEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC
QLVRITVDRYEAAAAVPKIRERLERLGFVIPLVGDFHYIGHKLLSEHPACAEALAKYRIN
CEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PGNVGFGAKKDRQFAEIIEIACRYRKPIRIGVNWGSLDHVLLTRLMDENAQQENPLSGGE
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHH
IMREAVVQSALHSALWAEEMGLKRDQIILSAKVSDVQDLIAVYSTLAERCDYALHLGLTE
HHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
AGMGTKGIVASSVALGILLQKGIGDTIRISLTPEPGGDRTREVKVAQELLQVMGFRQFFP
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VVTACPGCGRTTSTVFQQLAQKIETDLHKNMSVWREKYPGVESLKVAVMGCIVNGPGESK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCC
HADIGISLPGTGESPAAPVFIEGQKVKTLRGTHIAEEFELLLSDYIHTRFGQG
CCEEEEECCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA