Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 49476148

GI number: 49476148

Start: 1710791

End: 1711300

Strand: Reverse

Name: ruvC

Synonym: BH14910

Alternate gene names: 49476148

Gene position: 1711300-1710791 (Counterclockwise)

Preceding gene: 49476149

Following gene: 49476147

Centisome position: 88.62

GC content: 45.29

Gene sequence:

>510_bases
ATGGCAGAGACGATTCGTATTATAGGCATTGATCCAGGATTACGGCGAACAGGATGGGGGGTTATCGATTTCTCTGGCAA
TCGTCTTCAATTTGTTGCAGCGGGTACGCTTACTTCTGATGCACGTTGTGATCTTGCTTCTAGGCTCTGCCAGCTTTACA
AAGGGCTTACAGATATTTTGCATCAGTTTATGCCTCAGGAAGCCGCCGTAGAACATGTGTTCGTCAACAAGGATGCTACG
GCAACTTTAAAACTTGGTCAAGCACGGGCCATTGCACTTCTTGCTCCAGCGCAGGCAAATTTGCCTGTTTTTGAATATGC
TCCAAACAAAGTGAAAAAATCAGTTATTGGAGTTGGTCATGGCGATAAGGAACAAATTCACATGATGGTGAAAGTTCTTC
TTCCACGTGCTGAATTTGATAGCAGTGATGCCGCTGATGCACTTGCACTTGCTCTTTGTCACAGTACTCACCGTATCGGC
GCTTCTTATCATGCAAGGATTATGGCATGA

Upstream 100 bases:

>100_bases
GACAATCCGATAAGCAAACAAATCGAATTCTATCTTTCTTTCGTGTTCTTTGCTTTCGTGCATCTATTCAAATACGGAAA
AAGTGTATAAACTTGGCTTT

Downstream 100 bases:

>100_bases
TTGGCAAATTAAAAGGTATCCTTGAACACATCTTTGATGATCATATCATTGTGAATGTTCAGGGTGTGGGCTATGTAGTT
TTTATTTCAAATCGCCTGCG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAETIRIIGIDPGLRRTGWGVIDFSGNRLQFVAAGTLTSDARCDLASRLCQLYKGLTDILHQFMPQEAAVEHVFVNKDAT
ATLKLGQARAIALLAPAQANLPVFEYAPNKVKKSVIGVGHGDKEQIHMMVKVLLPRAEFDSSDAADALALALCHSTHRIG
ASYHARIMA

Sequences:

>Translated_169_residues
MAETIRIIGIDPGLRRTGWGVIDFSGNRLQFVAAGTLTSDARCDLASRLCQLYKGLTDILHQFMPQEAAVEHVFVNKDAT
ATLKLGQARAIALLAPAQANLPVFEYAPNKVKKSVIGVGHGDKEQIHMMVKVLLPRAEFDSSDAADALALALCHSTHRIG
ASYHARIMA
>Mature_168_residues
AETIRIIGIDPGLRRTGWGVIDFSGNRLQFVAAGTLTSDARCDLASRLCQLYKGLTDILHQFMPQEAAVEHVFVNKDATA
TLKLGQARAIALLAPAQANLPVFEYAPNKVKKSVIGVGHGDKEQIHMMVKVLLPRAEFDSSDAADALALALCHSTHRIGA
SYHARIMA

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=128, Percent_Identity=46.875, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_BARHE (Q6G5Q9)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034189.1
- ProteinModelPortal:   Q6G5Q9
- SMR:   Q6G5Q9
- GeneID:   2864586
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH14910
- NMPDR:   fig|283166.1.peg.1317
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q6G5Q9
- ProtClustDB:   PRK00039
- BioCyc:   BHEN283166:BH14910-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18290; Mature: 18159

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETIRIIGIDPGLRRTGWGVIDFSGNRLQFVAAGTLTSDARCDLASRLCQLYKGLTDIL
CCCEEEEEECCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
HQFMPQEAAVEHVFVNKDATATLKLGQARAIALLAPAQANLPVFEYAPNKVKKSVIGVGH
HHHCCHHHHHHEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEECCCHHHHHHHCCCCC
GDKEQIHMMVKVLLPRAEFDSSDAADALALALCHSTHRIGASYHARIMA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
AETIRIIGIDPGLRRTGWGVIDFSGNRLQFVAAGTLTSDARCDLASRLCQLYKGLTDIL
CCEEEEEECCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
HQFMPQEAAVEHVFVNKDATATLKLGQARAIALLAPAQANLPVFEYAPNKVKKSVIGVGH
HHHCCHHHHHHEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEECCCHHHHHHHCCCCC
GDKEQIHMMVKVLLPRAEFDSSDAADALALALCHSTHRIGASYHARIMA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA