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The map label for this gene is ruvA

Identifier: 49476147

GI number: 49476147

Start: 1710177

End: 1710794

Strand: Reverse

Name: ruvA

Synonym: BH14900

Alternate gene names: 49476147

Gene position: 1710794-1710177 (Counterclockwise)

Preceding gene: 49476148

Following gene: 49476146

Centisome position: 88.59

GC content: 42.39

Gene sequence:

>618_bases
ATGATTGGCAAATTAAAAGGTATCCTTGAACACATCTTTGATGATCATATCATTGTGAATGTTCAGGGTGTGGGCTATGT
AGTTTTTATTTCAAATCGCCTGCGTCCTTCTTTACCAGCTTTGGGGGAGAGTTTAAGTTTGTTTATTGAAACGCATGTTC
GTGAAGAAGCCATCCGCCTTTTTGGTTTTATTGCGAAAGCGGAACAAGAATGGTTTTGCTTATTACAAAATGTGCCTGGT
GTAGGAGCAAAAGTTGCTTTGGCAATTTTGGGAACTTTATCGCCAGATGAACTGGCACAGGCGATTGCCTTAAACGATAT
TGCAATGATAAGTCGTGCTCCAGGCGTTGGTAAAAAAGTCAGTGAAAGAATTGTGAGCGAACTGAAAAGCAAAGCTCTCC
CTTTTAGTGAAAAGGTGATCCATAGCGCTCCACTCCCTCATTTGGAGGTGACCAATCAACCTACAAATGATGCACTTTCC
GCTTTGATTAAGCTTGGTTTTGAACGTGATCAAGCAGCTAGAGCTCTTGCTTTAGCGGTGAACGCTCTTGAAGGGGAGAC
TGTTTCCTCTGCATTGCTTATTCGCCATAGCCTCAAATTGCTTTCTCCTCCTACTTAG

Upstream 100 bases:

>100_bases
GTGCTGAATTTGATAGCAGTGATGCCGCTGATGCACTTGCACTTGCTCTTTGTCACAGTACTCACCGTATCGGCGCTTCT
TATCATGCAAGGATTATGGC

Downstream 100 bases:

>100_bases
CGCAAGGTCACAATGCATAAAGATGAAGATCAACGCCTTTTGGGTGCCGTCCCCCTTCCTAATGATCCAGATCGTTCCTT
AAGACCACAAGTGCTTGATG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MIGKLKGILEHIFDDHIIVNVQGVGYVVFISNRLRPSLPALGESLSLFIETHVREEAIRLFGFIAKAEQEWFCLLQNVPG
VGAKVALAILGTLSPDELAQAIALNDIAMISRAPGVGKKVSERIVSELKSKALPFSEKVIHSAPLPHLEVTNQPTNDALS
ALIKLGFERDQAARALALAVNALEGETVSSALLIRHSLKLLSPPT

Sequences:

>Translated_205_residues
MIGKLKGILEHIFDDHIIVNVQGVGYVVFISNRLRPSLPALGESLSLFIETHVREEAIRLFGFIAKAEQEWFCLLQNVPG
VGAKVALAILGTLSPDELAQAIALNDIAMISRAPGVGKKVSERIVSELKSKALPFSEKVIHSAPLPHLEVTNQPTNDALS
ALIKLGFERDQAARALALAVNALEGETVSSALLIRHSLKLLSPPT
>Mature_205_residues
MIGKLKGILEHIFDDHIIVNVQGVGYVVFISNRLRPSLPALGESLSLFIETHVREEAIRLFGFIAKAEQEWFCLLQNVPG
VGAKVALAILGTLSPDELAQAIALNDIAMISRAPGVGKKVSERIVSELKSKALPFSEKVIHSAPLPHLEVTNQPTNDALS
ALIKLGFERDQAARALALAVNALEGETVSSALLIRHSLKLLSPPT

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=207, Percent_Identity=31.8840579710145, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BARHE (Q6G5R0)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034188.1
- ProteinModelPortal:   Q6G5R0
- SMR:   Q6G5R0
- GeneID:   2864584
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH14900
- NMPDR:   fig|283166.1.peg.1316
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- BioCyc:   BHEN283166:BH14900-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22093; Mature: 22093

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKLKGILEHIFDDHIIVNVQGVGYVVFISNRLRPSLPALGESLSLFIETHVREEAIRL
CCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FGFIAKAEQEWFCLLQNVPGVGAKVALAILGTLSPDELAQAIALNDIAMISRAPGVGKKV
HHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SERIVSELKSKALPFSEKVIHSAPLPHLEVTNQPTNDALSALIKLGFERDQAARALALAV
HHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
NALEGETVSSALLIRHSLKLLSPPT
HHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIGKLKGILEHIFDDHIIVNVQGVGYVVFISNRLRPSLPALGESLSLFIETHVREEAIRL
CCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FGFIAKAEQEWFCLLQNVPGVGAKVALAILGTLSPDELAQAIALNDIAMISRAPGVGKKV
HHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SERIVSELKSKALPFSEKVIHSAPLPHLEVTNQPTNDALSALIKLGFERDQAARALALAV
HHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
NALEGETVSSALLIRHSLKLLSPPT
HHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA