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The map label for this gene is tolB

Identifier: 49476141

GI number: 49476141

Start: 1704465

End: 1705796

Strand: Reverse

Name: tolB

Synonym: BH14840

Alternate gene names: 49476141

Gene position: 1705796-1704465 (Counterclockwise)

Preceding gene: 49476142

Following gene: 49476140

Centisome position: 88.34

GC content: 41.52

Gene sequence:

>1332_bases
ATGATGATTATGACGACACGCACATTTTTTTCTTGGTTTATTGTCATTTGTGCTTTTTGGCTTACAAGTTTTTCATCTGT
TCCTGTTCATGCACAACTGAAAGGCACTATTTCAACGGCTGATTTTAATCCCATTCCCATTGCGGTTACAGACTTTTTTT
CCAACGATTCCATTGGACTGAAGATTGCAGCGGTGGTGGCAGCAGATCTTGAACGGTCAGGACTTTTTTTACCGCTTGAT
AAGGCCTCTTTTTTAGAAAAAATCTCCAACCCTAACCGTCAACCCCATTTTCCTTATTGGCAAGAAATAAAAGCCCAAGG
TTTAGTCATTGGGCAAGTGATAAGAGAAAATGACGGACGCTTGAGAGTCGATTTTCGTTTATGGGATGTCTTTGGACAGC
GACAACTGAAAGGACGACGCTTTTATACAGCGACAGAACGTTGGCGGCGTGTCGCGCATATGATTGCAGATGAGATCTAT
AGTGAGATGACCGGAGAAAGTGGTTATTTTGATACACGGATTGTTTTTATCGATGAAACAGGTCCACAAAATGCACGTAT
CAAACGCTTAGCAATTATGGATCAAGATGGAGCAAATTTGATCTACATTTCTGATGGGAGTGAATTGGTGCTTACACCGC
GTTTTTCACCCAAACGGCAAGAAATTACTTATATGGCTTACGAACATAATCAAGTGCCTCATGTTTATCTTCAACAAATT
GAAATGGGACAAAGGGAGTTGATAGGAACTTTTAACAATATGACAATTGCTCCGCGTTTTTCTTCTGATGGACAAAAAGT
GATCATGAGTTTGTTGCAAAATGATGGGAGCGCAAATCTTTACACTATGGATTTACGTACACGCATGATGACGCGTCTTA
CCACAACTTCTGCCATTGATACTTCTGCTTCTTATTCGCCAGATGGGACAAAAATCGTCTTTTCATCGGATCGCAGTGGA
AAGCCACAAATCTATACGATGAATGCCGATGGGAGCAATCTCCAGCGTATTTCTTCAAACGAGGGCAGTTATTCTACACC
CATTTGGTCTCCACGTGGTGATTATATTGCTTTTACAAAACAATTGGAGGGACAATTTTCTATTGGCGTTATGCATCCTG
ATGGACAAGGAGAACGAATTTTAACCACCGGTTTTCACAATGAAGGACCAACTTGGGCTCCTAATGGCCGCGTGTTGATG
TTCTTTCGCAAAAATCCGGGTATGGGGCCTAAAATCTACACAATTGACATTACTGGACGCAATGAACGTCAATTGCCCAC
ACCTAATGATGCTTCTGATCCAGCATGGTCCCCATTGCTCAATATACAATAA

Upstream 100 bases:

>100_bases
TTATGGGGACAGGGTTTTGATTTTAATGTTGATCCCCTTCAAGAGATAATACGATGATATATACTTCATTTTTCGCTCTT
CAGTAAAAGGATACACATCC

Downstream 100 bases:

>100_bases
AGGCAACTCTTTCATAAAAAAACAGCAGATAAGCAATGCGCTCAGAAAGCGTAACTGTAGCTATAACTTAAAAAAAGGGG
GATATTGCTTTCGATAATTG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MMIMTTRTFFSWFIVICAFWLTSFSSVPVHAQLKGTISTADFNPIPIAVTDFFSNDSIGLKIAAVVAADLERSGLFLPLD
KASFLEKISNPNRQPHFPYWQEIKAQGLVIGQVIRENDGRLRVDFRLWDVFGQRQLKGRRFYTATERWRRVAHMIADEIY
SEMTGESGYFDTRIVFIDETGPQNARIKRLAIMDQDGANLIYISDGSELVLTPRFSPKRQEITYMAYEHNQVPHVYLQQI
EMGQRELIGTFNNMTIAPRFSSDGQKVIMSLLQNDGSANLYTMDLRTRMMTRLTTTSAIDTSASYSPDGTKIVFSSDRSG
KPQIYTMNADGSNLQRISSNEGSYSTPIWSPRGDYIAFTKQLEGQFSIGVMHPDGQGERILTTGFHNEGPTWAPNGRVLM
FFRKNPGMGPKIYTIDITGRNERQLPTPNDASDPAWSPLLNIQ

Sequences:

>Translated_443_residues
MMIMTTRTFFSWFIVICAFWLTSFSSVPVHAQLKGTISTADFNPIPIAVTDFFSNDSIGLKIAAVVAADLERSGLFLPLD
KASFLEKISNPNRQPHFPYWQEIKAQGLVIGQVIRENDGRLRVDFRLWDVFGQRQLKGRRFYTATERWRRVAHMIADEIY
SEMTGESGYFDTRIVFIDETGPQNARIKRLAIMDQDGANLIYISDGSELVLTPRFSPKRQEITYMAYEHNQVPHVYLQQI
EMGQRELIGTFNNMTIAPRFSSDGQKVIMSLLQNDGSANLYTMDLRTRMMTRLTTTSAIDTSASYSPDGTKIVFSSDRSG
KPQIYTMNADGSNLQRISSNEGSYSTPIWSPRGDYIAFTKQLEGQFSIGVMHPDGQGERILTTGFHNEGPTWAPNGRVLM
FFRKNPGMGPKIYTIDITGRNERQLPTPNDASDPAWSPLLNIQ
>Mature_443_residues
MMIMTTRTFFSWFIVICAFWLTSFSSVPVHAQLKGTISTADFNPIPIAVTDFFSNDSIGLKIAAVVAADLERSGLFLPLD
KASFLEKISNPNRQPHFPYWQEIKAQGLVIGQVIRENDGRLRVDFRLWDVFGQRQLKGRRFYTATERWRRVAHMIADEIY
SEMTGESGYFDTRIVFIDETGPQNARIKRLAIMDQDGANLIYISDGSELVLTPRFSPKRQEITYMAYEHNQVPHVYLQQI
EMGQRELIGTFNNMTIAPRFSSDGQKVIMSLLQNDGSANLYTMDLRTRMMTRLTTTSAIDTSASYSPDGTKIVFSSDRSG
KPQIYTMNADGSNLQRISSNEGSYSTPIWSPRGDYIAFTKQLEGQFSIGVMHPDGQGERILTTGFHNEGPTWAPNGRVLM
FFRKNPGMGPKIYTIDITGRNERQLPTPNDASDPAWSPLLNIQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=418, Percent_Identity=29.1866028708134, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_BARHE (Q6G5R6)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034182.1
- ProteinModelPortal:   Q6G5R6
- SMR:   Q6G5R6
- GeneID:   2865231
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH14840
- NMPDR:   fig|283166.1.peg.1310
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q6G5R6
- ProtClustDB:   PRK05137
- BioCyc:   BHEN283166:BH14840-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 50142; Mature: 50142

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIMTTRTFFSWFIVICAFWLTSFSSVPVHAQLKGTISTADFNPIPIAVTDFFSNDSIGL
CEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCE
KIAAVVAADLERSGLFLPLDKASFLEKISNPNRQPHFPYWQEIKAQGLVIGQVIRENDGR
EEEEEEEEHHHHCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCE
LRVDFRLWDVFGQRQLKGRRFYTATERWRRVAHMIADEIYSEMTGESGYFDTRIVFIDET
EEEEEEEEEHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
GPQNARIKRLAIMDQDGANLIYISDGSELVLTPRFSPKRQEITYMAYEHNQVPHVYLQQI
CCCCCEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHH
EMGQRELIGTFNNMTIAPRFSSDGQKVIMSLLQNDGSANLYTMDLRTRMMTRLTTTSAID
HCCCHHHHEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCC
TSASYSPDGTKIVFSSDRSGKPQIYTMNADGSNLQRISSNEGSYSTPIWSPRGDYIAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHEECCCCCCCCCCCCCCCCCEEEEEE
QLEGQFSIGVMHPDGQGERILTTGFHNEGPTWAPNGRVLMFFRKNPGMGPKIYTIDITGR
ECCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCC
NERQLPTPNDASDPAWSPLLNIQ
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMIMTTRTFFSWFIVICAFWLTSFSSVPVHAQLKGTISTADFNPIPIAVTDFFSNDSIGL
CEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCE
KIAAVVAADLERSGLFLPLDKASFLEKISNPNRQPHFPYWQEIKAQGLVIGQVIRENDGR
EEEEEEEEHHHHCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCE
LRVDFRLWDVFGQRQLKGRRFYTATERWRRVAHMIADEIYSEMTGESGYFDTRIVFIDET
EEEEEEEEEHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
GPQNARIKRLAIMDQDGANLIYISDGSELVLTPRFSPKRQEITYMAYEHNQVPHVYLQQI
CCCCCEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHH
EMGQRELIGTFNNMTIAPRFSSDGQKVIMSLLQNDGSANLYTMDLRTRMMTRLTTTSAID
HCCCHHHHEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCC
TSASYSPDGTKIVFSSDRSGKPQIYTMNADGSNLQRISSNEGSYSTPIWSPRGDYIAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHEECCCCCCCCCCCCCCCCCEEEEEE
QLEGQFSIGVMHPDGQGERILTTGFHNEGPTWAPNGRVLMFFRKNPGMGPKIYTIDITGR
ECCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCC
NERQLPTPNDASDPAWSPLLNIQ
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA