Click here to switch to the map view.

The map label for this gene is tilS

Identifier: 49476135

GI number: 49476135

Start: 1696085

End: 1697566

Strand: Reverse

Name: tilS

Synonym: BH14760

Alternate gene names: 49476135

Gene position: 1697566-1696085 (Counterclockwise)

Preceding gene: 49476136

Following gene: 49476134

Centisome position: 87.91

GC content: 43.05

Gene sequence:

>1482_bases
GTGTGCGTTAGACTAGCAGGAAATCTCTTTAAAACATCAGATTTTATTCAGTGTCAGAAGTTGATACTTGCAGTCTCAGG
CGGAAGTGATTCTCTTGCTTTGCTATTTCTGGTCAAGGACCATTTAAAAACGCTCTCTGTTCCTCCGGAAATAATTGTCG
TGACCGTTGATCATCAATTGCGTCAAGAATCAGCGCGTGAAGCTTCAATTGTTGCGGAAATTTGTCGTGCTCATCATATT
CAACATCGCATTGTGCGCTGGGAGGGTAAAAAGCCAAAGACCCATATCGCTTCAAGCGCTCGTGTTGCACGCTATGATCT
GCTCTTTCAAGAAGCGCAAAAACAAGGCGCAACACTTATTATGACAGGGCATACGCTTAATGATCAGGTTGAAACTTATC
AAATGCGGTATCAACGTCTCCAAAAGAGCGCGGATGTTTTGCAGCAGGAAGCTTTTGCAGAAATGGGTGGGGGAGTTCAT
GCGGATGTTTTGCAGCAGGAAGCCTTTGCAGAGAGGTGCGAGAGGATGGGTGTTGGAGGTCATGGAGGAGATATTGCCGA
AAAAAGTGATGGGCTGATCTATGAGCGCGGTTTATCTTGTATTCCGCGTGAAGCACTGTTGCGTGGAACAGTGCGTTTAA
TTCGTCCGCTCCTTGGTGTAAAGCGTGAAACATTGCGGACGTATCTACGTTTAAAAGAAAAAACATGGATTGAAGATCCA
ACCAATGAAGATTGCAATTTTGAACGTGTGCGTGTGCGTCAATCCCTTCAACCCAAAAAGTTTACCTGCATCGCGCGAAA
AGTTCATGAAGCGGCGCTTCAACGTCGTCAACAGGCAAAAAATATTGCGGATCTGATTTTGGCTCTGGATATCACTGTTG
AATATGGGCGTTGTTTTATTGCAAAGCCTGCGTTATTCTTACAGAAACATCCAGGTTTTCCTTTTGTCGTGGGGCTTTTC
GCTGTTTTGATGGGAGGCGGCTTTTACTTGCTCCCTCATAAAAAATTAAGCACTCTCGTTCAAAAATTATCTCTCCATTC
TTCAGAAAAACGCCGCTTTACTTATGCTGGATCTGTCATTGAATATAACAGAAGCGGAATCGCTTTTTGGCGTGAAGCGC
GAAATATCAAAGAAGCTATTGTTGAAAAAGGACAAACTTTTTTATGGGATGGGCGCTATCAGATCACAAATCATAGCCAT
GAGCCCATAAAAGTTGGAGCAGCGGGTTTGCAACAGTTGAAATCTCTCTTCAAAAACAACAATTTCAATCTTGAAAACAC
CCATTTCCCTTCTCTAAAATCCCTGTTGATGATCTCAAATGATAAGGGGTACGATATTCCAGAATTGGCTTATCACGCTG
CTATGCAACACACTATCACCATAAGACGCATTATGGCTCCCTTCGATTGGCTTTTGTCTTCTCAAGATGCTGCTTTTGTG
AATGTTGTGCAGCCTTTTTTTGATATTAAGGTGAAAGGATAA

Upstream 100 bases:

>100_bases
AATAAAGAGTTTTGTTCACTTTTTGTAGAAAGATCAAAGCATCAGAAAATGTTGGAGTCTGTTTTTTGTAAACCTCTAAA
AAGGAAAGGAGTCGTTTACG

Downstream 100 bases:

>100_bases
AGAAAAATCACTCTAAAAGAGAATAAACATCCTCTCATATCTTGGCAAGGAGCTGACAAGGTTATATGTTAGGGGTAACT
ATTTAGGATCTGTATTTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MCVRLAGNLFKTSDFIQCQKLILAVSGGSDSLALLFLVKDHLKTLSVPPEIIVVTVDHQLRQESAREASIVAEICRAHHI
QHRIVRWEGKKPKTHIASSARVARYDLLFQEAQKQGATLIMTGHTLNDQVETYQMRYQRLQKSADVLQQEAFAEMGGGVH
ADVLQQEAFAERCERMGVGGHGGDIAEKSDGLIYERGLSCIPREALLRGTVRLIRPLLGVKRETLRTYLRLKEKTWIEDP
TNEDCNFERVRVRQSLQPKKFTCIARKVHEAALQRRQQAKNIADLILALDITVEYGRCFIAKPALFLQKHPGFPFVVGLF
AVLMGGGFYLLPHKKLSTLVQKLSLHSSEKRRFTYAGSVIEYNRSGIAFWREARNIKEAIVEKGQTFLWDGRYQITNHSH
EPIKVGAAGLQQLKSLFKNNNFNLENTHFPSLKSLLMISNDKGYDIPELAYHAAMQHTITIRRIMAPFDWLLSSQDAAFV
NVVQPFFDIKVKG

Sequences:

>Translated_493_residues
MCVRLAGNLFKTSDFIQCQKLILAVSGGSDSLALLFLVKDHLKTLSVPPEIIVVTVDHQLRQESAREASIVAEICRAHHI
QHRIVRWEGKKPKTHIASSARVARYDLLFQEAQKQGATLIMTGHTLNDQVETYQMRYQRLQKSADVLQQEAFAEMGGGVH
ADVLQQEAFAERCERMGVGGHGGDIAEKSDGLIYERGLSCIPREALLRGTVRLIRPLLGVKRETLRTYLRLKEKTWIEDP
TNEDCNFERVRVRQSLQPKKFTCIARKVHEAALQRRQQAKNIADLILALDITVEYGRCFIAKPALFLQKHPGFPFVVGLF
AVLMGGGFYLLPHKKLSTLVQKLSLHSSEKRRFTYAGSVIEYNRSGIAFWREARNIKEAIVEKGQTFLWDGRYQITNHSH
EPIKVGAAGLQQLKSLFKNNNFNLENTHFPSLKSLLMISNDKGYDIPELAYHAAMQHTITIRRIMAPFDWLLSSQDAAFV
NVVQPFFDIKVKG
>Mature_493_residues
MCVRLAGNLFKTSDFIQCQKLILAVSGGSDSLALLFLVKDHLKTLSVPPEIIVVTVDHQLRQESAREASIVAEICRAHHI
QHRIVRWEGKKPKTHIASSARVARYDLLFQEAQKQGATLIMTGHTLNDQVETYQMRYQRLQKSADVLQQEAFAEMGGGVH
ADVLQQEAFAERCERMGVGGHGGDIAEKSDGLIYERGLSCIPREALLRGTVRLIRPLLGVKRETLRTYLRLKEKTWIEDP
TNEDCNFERVRVRQSLQPKKFTCIARKVHEAALQRRQQAKNIADLILALDITVEYGRCFIAKPALFLQKHPGFPFVVGLF
AVLMGGGFYLLPHKKLSTLVQKLSLHSSEKRRFTYAGSVIEYNRSGIAFWREARNIKEAIVEKGQTFLWDGRYQITNHSH
EPIKVGAAGLQQLKSLFKNNNFNLENTHFPSLKSLLMISNDKGYDIPELAYHAAMQHTITIRRIMAPFDWLLSSQDAAFV
NVVQPFFDIKVKG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BARHE (Q6G5P5)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034176.1
- ProteinModelPortal:   Q6G5P5
- GeneID:   2865727
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH14760
- NMPDR:   fig|283166.1.peg.1304
- HOGENOM:   HBG348374
- OMA:   DDQRETL
- ProtClustDB:   CLSK927306
- BioCyc:   BHEN283166:BH14760-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 55900; Mature: 55900

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVRLAGNLFKTSDFIQCQKLILAVSGGSDSLALLFLVKDHLKTLSVPPEIIVVTVDHQL
CEEEECCCHHCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHH
RQESAREASIVAEICRAHHIQHRIVRWEGKKPKTHIASSARVARYDLLFQEAQKQGATLI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEE
MTGHTLNDQVETYQMRYQRLQKSADVLQQEAFAEMGGGVHADVLQQEAFAERCERMGVGG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGDIAEKSDGLIYERGLSCIPREALLRGTVRLIRPLLGVKRETLRTYLRLKEKTWIEDP
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
TNEDCNFERVRVRQSLQPKKFTCIARKVHEAALQRRQQAKNIADLILALDITVEYGRCFI
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEE
AKPALFLQKHPGFPFVVGLFAVLMGGGFYLLPHKKLSTLVQKLSLHSSEKRRFTYAGSVI
ECCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEECCEE
EYNRSGIAFWREARNIKEAIVEKGQTFLWDGRYQITNHSHEPIKVGAAGLQQLKSLFKNN
EECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCEEHHHHHHHHHHHHHCCC
NFNLENTHFPSLKSLLMISNDKGYDIPELAYHAAMQHTITIRRIMAPFDWLLSSQDAAFV
CCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NVVQPFFDIKVKG
HHHHHHHCEEECC
>Mature Secondary Structure
MCVRLAGNLFKTSDFIQCQKLILAVSGGSDSLALLFLVKDHLKTLSVPPEIIVVTVDHQL
CEEEECCCHHCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHH
RQESAREASIVAEICRAHHIQHRIVRWEGKKPKTHIASSARVARYDLLFQEAQKQGATLI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEE
MTGHTLNDQVETYQMRYQRLQKSADVLQQEAFAEMGGGVHADVLQQEAFAERCERMGVGG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGDIAEKSDGLIYERGLSCIPREALLRGTVRLIRPLLGVKRETLRTYLRLKEKTWIEDP
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
TNEDCNFERVRVRQSLQPKKFTCIARKVHEAALQRRQQAKNIADLILALDITVEYGRCFI
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEE
AKPALFLQKHPGFPFVVGLFAVLMGGGFYLLPHKKLSTLVQKLSLHSSEKRRFTYAGSVI
ECCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEECCEE
EYNRSGIAFWREARNIKEAIVEKGQTFLWDGRYQITNHSHEPIKVGAAGLQQLKSLFKNN
EECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCEEHHHHHHHHHHHHHCCC
NFNLENTHFPSLKSLLMISNDKGYDIPELAYHAAMQHTITIRRIMAPFDWLLSSQDAAFV
CCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NVVQPFFDIKVKG
HHHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA