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The map label for this gene is pcnB [H]

Identifier: 49475914

GI number: 49475914

Start: 1325544

End: 1326797

Strand: Reverse

Name: pcnB [H]

Synonym: BH11930

Alternate gene names: 49475914

Gene position: 1326797-1325544 (Counterclockwise)

Preceding gene: 49475920

Following gene: 49475912

Centisome position: 68.71

GC content: 40.43

Gene sequence:

>1254_bases
GTGAGCATAAGACAAGATTTCAAACAGGTTGATTGGTTGCAGCATAGTCATATCCAAAAACTGCTTCATGTTTTATCTTT
AGACGGTGAAGAGGCGCGTATTGTGGGGGGGGCGGTACGTAATCAGCTGTTAGAGCAACCTATTAGTGATGTTGATATTG
CCACAACCTGTTTGCCACAACAGATTATAGTGCGTGTAGAAGAAGCAGGATTTAAAGCTATTCCTACTGGTATTGCTTTT
GGAACAGTTACCGTGGTTTCTCAGTCATGTTCTTACGAGGTTACAACTCTTCGCTCTGATATTGAAACGGATGGTCGTCA
TGCAAAAGTGGCCTTCGGTCGTGATTGGCAAAAAGATGCTGAACGGCGTGATTTTACTATGAATGCGCTTTATTGTGATG
CTGCTGGCAACCTTTATGATGCAGTGGGAGGCCTGGACGATATTGCCAGCAGAACAGTACGTTTTATCGGTATTGCAGAG
AAGCGTATTCGTGAAGATTATCTGCGTATTTTGCGGTTTTTTCGCTTTTTTGCTTGGTATGGGGCGGGACGACCTGATGT
ACAAGGGTTGAAAGCATGCGTTTGTTTGAAACACGGTCTGCAAAAACTCTCATCTGAGCGTATTTGGGGGGAAATGAAAA
AACTTCTCACTGCGCCTAATCCTATACGTGCTCTTCTATGGATGCGCCAAAGCGGAATTTTGACACTCATTTTTCCTGAA
ACAGAAAAATGGGGGATTGATGCGATTCATTCACTTGTGAAAACAGAACAGGATCTTGGTTGGAAAGTAGATCCATTGTT
GCGATTAGAAAGTCTTCTTCCTCCTGATCCTGTACGTCTTTATGAAATGGCGCATCGCTTGCCTTTATCTCATAAGGAAA
AAACGCGGTTAAAAGAATGGGCAGAGTTGGAAATTATTTGCCAAAACTGTTCAGATCATTTTTTGCAAAAATTGATTTAT
TTTCATGGTCGACAACCGGTTTTAGATCAATTGTCCCTCTCTTTGGCCGCAGCCCATGTTGATGTTTTAAAGGGAAATGA
AGCTTTGCAAAAAGTAGAAGATTATATGAGGCTTTATCAGTTTGCTCAAAAATGGCAGATTCCAACTTTTCCTATTAGTG
GTAAAGATTTGATGAAGAAAGGTTTTTCTAAAGGCGTGCTTTTAGGACAAAAGCTCAAAGAACTAGAAACACTATGGATT
GAAAGCGGATTTTTAATAGATCGCAATAGCTTATTAGAAAAAATTAATTTATAA

Upstream 100 bases:

>100_bases
AGGAGTGCTGTGTATCTTATAAAGGATGTCGTGGAAAACTATTGTCCTATAAAGATACCAGTTTGAGAGAATGATGGCGA
TTTGTTTGAAAGGTTAAAGA

Downstream 100 bases:

>100_bases
TATTAATATTTTCGAAGAGTTTTTTTCTTTTAATGATGCCCCTTTAGAGTTTTTGGGAGCAAAAAGAGTATGAAATTTTT
AGGAGTATTACGACGTTTTT

Product: Poly(a) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSIRQDFKQVDWLQHSHIQKLLHVLSLDGEEARIVGGAVRNQLLEQPISDVDIATTCLPQQIIVRVEEAGFKAIPTGIAF
GTVTVVSQSCSYEVTTLRSDIETDGRHAKVAFGRDWQKDAERRDFTMNALYCDAAGNLYDAVGGLDDIASRTVRFIGIAE
KRIREDYLRILRFFRFFAWYGAGRPDVQGLKACVCLKHGLQKLSSERIWGEMKKLLTAPNPIRALLWMRQSGILTLIFPE
TEKWGIDAIHSLVKTEQDLGWKVDPLLRLESLLPPDPVRLYEMAHRLPLSHKEKTRLKEWAELEIICQNCSDHFLQKLIY
FHGRQPVLDQLSLSLAAAHVDVLKGNEALQKVEDYMRLYQFAQKWQIPTFPISGKDLMKKGFSKGVLLGQKLKELETLWI
ESGFLIDRNSLLEKINL

Sequences:

>Translated_417_residues
MSIRQDFKQVDWLQHSHIQKLLHVLSLDGEEARIVGGAVRNQLLEQPISDVDIATTCLPQQIIVRVEEAGFKAIPTGIAF
GTVTVVSQSCSYEVTTLRSDIETDGRHAKVAFGRDWQKDAERRDFTMNALYCDAAGNLYDAVGGLDDIASRTVRFIGIAE
KRIREDYLRILRFFRFFAWYGAGRPDVQGLKACVCLKHGLQKLSSERIWGEMKKLLTAPNPIRALLWMRQSGILTLIFPE
TEKWGIDAIHSLVKTEQDLGWKVDPLLRLESLLPPDPVRLYEMAHRLPLSHKEKTRLKEWAELEIICQNCSDHFLQKLIY
FHGRQPVLDQLSLSLAAAHVDVLKGNEALQKVEDYMRLYQFAQKWQIPTFPISGKDLMKKGFSKGVLLGQKLKELETLWI
ESGFLIDRNSLLEKINL
>Mature_416_residues
SIRQDFKQVDWLQHSHIQKLLHVLSLDGEEARIVGGAVRNQLLEQPISDVDIATTCLPQQIIVRVEEAGFKAIPTGIAFG
TVTVVSQSCSYEVTTLRSDIETDGRHAKVAFGRDWQKDAERRDFTMNALYCDAAGNLYDAVGGLDDIASRTVRFIGIAEK
RIREDYLRILRFFRFFAWYGAGRPDVQGLKACVCLKHGLQKLSSERIWGEMKKLLTAPNPIRALLWMRQSGILTLIFPET
EKWGIDAIHSLVKTEQDLGWKVDPLLRLESLLPPDPVRLYEMAHRLPLSHKEKTRLKEWAELEIICQNCSDHFLQKLIYF
HGRQPVLDQLSLSLAAAHVDVLKGNEALQKVEDYMRLYQFAQKWQIPTFPISGKDLMKKGFSKGVLLGQKLKELETLWIE
SGFLIDRNSLLEKINL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=408, Percent_Identity=33.578431372549, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1789436, Length=261, Percent_Identity=29.5019157088123, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081691, Length=212, Percent_Identity=33.0188679245283, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=405, Percent_Identity=33.0864197530864, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=30.1310043668122, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281360112, Length=389, Percent_Identity=33.9331619537275, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21357337, Length=389, Percent_Identity=33.9331619537275, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47651; Mature: 47520

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRQDFKQVDWLQHSHIQKLLHVLSLDGEEARIVGGAVRNQLLEQPISDVDIATTCLPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCH
QIIVRVEEAGFKAIPTGIAFGTVTVVSQSCSYEVTTLRSDIETDGRHAKVAFGRDWQKDA
HHHHHHHHCCCEECCCCHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECCCHHHHH
ERRDFTMNALYCDAAGNLYDAVGGLDDIASRTVRFIGIAEKRIREDYLRILRFFRFFAWY
HHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAGRPDVQGLKACVCLKHGLQKLSSERIWGEMKKLLTAPNPIRALLWMRQSGILTLIFPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECC
TEKWGIDAIHSLVKTEQDLGWKVDPLLRLESLLPPDPVRLYEMAHRLPLSHKEKTRLKEW
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
AELEIICQNCSDHFLQKLIYFHGRQPVLDQLSLSLAAAHVDVLKGNEALQKVEDYMRLYQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FAQKWQIPTFPISGKDLMKKGFSKGVLLGQKLKELETLWIESGFLIDRNSLLEKINL
HHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHHCC
>Mature Secondary Structure 
SIRQDFKQVDWLQHSHIQKLLHVLSLDGEEARIVGGAVRNQLLEQPISDVDIATTCLPQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCH
QIIVRVEEAGFKAIPTGIAFGTVTVVSQSCSYEVTTLRSDIETDGRHAKVAFGRDWQKDA
HHHHHHHHCCCEECCCCHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECCCHHHHH
ERRDFTMNALYCDAAGNLYDAVGGLDDIASRTVRFIGIAEKRIREDYLRILRFFRFFAWY
HHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAGRPDVQGLKACVCLKHGLQKLSSERIWGEMKKLLTAPNPIRALLWMRQSGILTLIFPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECC
TEKWGIDAIHSLVKTEQDLGWKVDPLLRLESLLPPDPVRLYEMAHRLPLSHKEKTRLKEW
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
AELEIICQNCSDHFLQKLIYFHGRQPVLDQLSLSLAAAHVDVLKGNEALQKVEDYMRLYQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FAQKWQIPTFPISGKDLMKKGFSKGVLLGQKLKELETLWIESGFLIDRNSLLEKINL
HHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA