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The map label for this gene is pcm2 [H]

Identifier: 49475809

GI number: 49475809

Start: 1172256

End: 1172930

Strand: Reverse

Name: pcm2 [H]

Synonym: BH10660

Alternate gene names: 49475809

Gene position: 1172930-1172256 (Counterclockwise)

Preceding gene: 49475810

Following gene: 49475808

Centisome position: 60.74

GC content: 41.63

Gene sequence:

>675_bases
ATGGTTGCAGATTTCGCAGAGCTTCGCCGGAAAATGGTCGACAATCAAATTCGTACAGTGGATGTGACGGATTTATCAGT
TCTTGAAGCGTTTTTAATGGTACCGCGTGAAGATTTTGTACCGGAAAATATGAAAGCTTTGAGTTATCTCGATACCGATA
TTCTTCTATATCCAGCGCAGGAGGGAGTTCCTGCGTGCTATTTGATGGGACCTGCGCCTTTAGCTAAATTGCTACAGCTT
GCCGCTGTAAAATCATCGGATATTGTGTTAGATATTGGGGCTAATGGTGGTTATTGTGCAGCATTGCTTTCAAAACTTGC
GCGTTCTGTTATTGCGTTAGAAGCTCATAAAGCTCTTTTAGAACGAACTGCCTCGACTTTAGAACGTAATCAATGTAACA
ATGTGGTTGTGGTTCATGGAGCATTGGAGCAGGGCTATGCCGTTGAGGGTCCTTATGATGTGATTTTTATTGAGGGTTCT
GTCGATTTTATCCCAAAGATTATCTTTGATCAGATGAAAGATGGGGGGCGTCTTGTTGTGGTTGAAGGACACGGTAACGC
AGGTGTTGCTCTAATCTATGTAAAAGAAGATGGAATTATTTCTGCGCGTCGTGCTTTTAATCTGGCTGTAAAACGTATTT
CTGGTTTTTTAAAGACGCCTGATTTTATATTTTAG

Upstream 100 bases:

>100_bases
CTCGTTGTTGACTGGAGCGTATTTTTGAAAACACCTTTATAAGGATTGTAGATCCTTATTATCGTTGTTTTGTTATTGTA
TGGAGGAGACAAGGGGCATT

Downstream 100 bases:

>100_bases
TGCTTGTTTTGTTTGTGAATAAAAGATAATTGTATGAATTTGGGGTTTTTTATTGTGTGTGTTGTGTTCAGAATGAAAAA
GAAACTTCAGGCGTGTTTCT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MVADFAELRRKMVDNQIRTVDVTDLSVLEAFLMVPREDFVPENMKALSYLDTDILLYPAQEGVPACYLMGPAPLAKLLQL
AAVKSSDIVLDIGANGGYCAALLSKLARSVIALEAHKALLERTASTLERNQCNNVVVVHGALEQGYAVEGPYDVIFIEGS
VDFIPKIIFDQMKDGGRLVVVEGHGNAGVALIYVKEDGIISARRAFNLAVKRISGFLKTPDFIF

Sequences:

>Translated_224_residues
MVADFAELRRKMVDNQIRTVDVTDLSVLEAFLMVPREDFVPENMKALSYLDTDILLYPAQEGVPACYLMGPAPLAKLLQL
AAVKSSDIVLDIGANGGYCAALLSKLARSVIALEAHKALLERTASTLERNQCNNVVVVHGALEQGYAVEGPYDVIFIEGS
VDFIPKIIFDQMKDGGRLVVVEGHGNAGVALIYVKEDGIISARRAFNLAVKRISGFLKTPDFIF
>Mature_224_residues
MVADFAELRRKMVDNQIRTVDVTDLSVLEAFLMVPREDFVPENMKALSYLDTDILLYPAQEGVPACYLMGPAPLAKLLQL
AAVKSSDIVLDIGANGGYCAALLSKLARSVIALEAHKALLERTASTLERNQCNNVVVVHGALEQGYAVEGPYDVIFIEGS
VDFIPKIIFDQMKDGGRLVVVEGHGNAGVALIYVKEDGIISARRAFNLAVKRISGFLKTPDFIF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=169, Percent_Identity=29.585798816568, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24415; Mature: 24415

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADFAELRRKMVDNQIRTVDVTDLSVLEAFLMVPREDFVPENMKALSYLDTDILLYPAQ
CCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEECC
EGVPACYLMGPAPLAKLLQLAAVKSSDIVLDIGANGGYCAALLSKLARSVIALEAHKALL
CCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERTASTLERNQCNNVVVVHGALEQGYAVEGPYDVIFIEGSVDFIPKIIFDQMKDGGRLVV
HHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCCCEEEE
VEGHGNAGVALIYVKEDGIISARRAFNLAVKRISGFLKTPDFIF
EECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVADFAELRRKMVDNQIRTVDVTDLSVLEAFLMVPREDFVPENMKALSYLDTDILLYPAQ
CCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEECC
EGVPACYLMGPAPLAKLLQLAAVKSSDIVLDIGANGGYCAALLSKLARSVIALEAHKALL
CCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERTASTLERNQCNNVVVVHGALEQGYAVEGPYDVIFIEGSVDFIPKIIFDQMKDGGRLVV
HHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCCCEEEE
VEGHGNAGVALIYVKEDGIISARRAFNLAVKRISGFLKTPDFIF
EECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA