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The map label for this gene is tuf [H]

Identifier: 49475796

GI number: 49475796

Start: 1156766

End: 1157941

Strand: Reverse

Name: tuf [H]

Synonym: BH10530

Alternate gene names: 49475796

Gene position: 1157941-1156766 (Counterclockwise)

Preceding gene: 49475797

Following gene: 49475795

Centisome position: 59.96

GC content: 44.3

Gene sequence:

>1176_bases
ATGGCGAAGAGCAAATTTGAACGCACGAAACCGCATGTTAATATTGGTACGATTGGTCACGTTGACCATGGGAAGACCTC
GTTGACGGCAGCGATTACGAAATATTTTGGTGAATTTAAGGCCTATGACCAAATTGATGCAGCGCCTGAGGAGCGTGCAC
GTGGAATTACTATTTCTACAGCGCATGTTGAATATGAAACAGAGAAGCGGCATTATGCACATGTTGATTGTCCAGGTCAC
GCGGATTATGTGAAGAACATGATCACGGGCGCGGCGCAAATGGATGGTGCGATTTTGGTTGTTTCAGCTGCTGATGGTCC
GATGCCTCAAACACGTGAGCATATTCTTCTTGCCCGTCAGGTTGGTGTTCCAGCGATTGTTGTTTTTCTTAATAAGGTTG
ATCAGGTTGATGATGCTGAGCTTTTGGAGCTTGTTGAGCTTGAAGTTCGGGAGTTATTGTCGAAATATGATTTTCCAGGA
GACGATATTCCGATCGTTAAAGGTTCTGCTTTGGCAGCGCTTGAAGATAAAGATAAAAGCATTGGTGAAGATGCGGTTCG
TCTTTTGATGAGTGAAGTTGATAATTATATACCGACGCCTGAACGTCCTGTTGATCAGCCGTTTTTGATGCCAATTGAAG
ATGTTTTTTCGATTTCGGGTCGTGGAACTGTTGTGACGGGTCGTGTTGAGCGTGGTGTTATTAAGGTTGGTGAAGAAGTT
GAGATTATCGGCATTCGTCCAACTTCTAAGACAACAGTTACAGGGGTTGAAATGTTCCGCAAGCTTTTAGATCAGGGGCA
AGCGGGTGATAATATTGGAGCGCTGCTTCGTGGTATTGATCGTGAAGGGATTGAGCGTGGACAAGTTTTGGCGAAGCCTG
CTTCGGTTACACCTCATACGAGATTTAAAGCAGAGGCTTACATTTTGACGAAAGATGAAGGTGGTCGTCATACTCCATTT
TTCACGAATTATCGTCCTCAGTTTTATTTCCGTACTACGGATGTAACGGGAATTGTTACGCTTCCAGAAGGTACAGAGAT
GGTTATGCCTGGTGATAATGTTGCTATGGATGTCTCTCTGATTGTTCCAATTGCCATGGAAGAAAAACTTCGTTTTGCTA
TCCGTGAAGGTGGTCGTACTGTTGGTGCAGGTATCGTTTCTAAGATCATTGAGTAA

Upstream 100 bases:

>100_bases
GCTGTTGCTTTGGAGATACAAAAGAAATACGCGTAATTTTAGGTAGCGTAATTAATTTTTAACTTTAGTCCTTGATTTGG
ACAGAAAATGGAGAGCTCAA

Downstream 100 bases:

>100_bases
ATAATGGTTGTTGAAAGAGCGCTTTTATTGTTAAAGGCGTTTTGCATAACAAGGAAATAATGAGCATGAACAGTCAAAAT
ATCCGCATTCGTTTGAAAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEERARGITISTAHVEYETEKRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSKYDFPG
DDIPIVKGSALAALEDKDKSIGEDAVRLLMSEVDNYIPTPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGVIKVGEEV
EIIGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGIERGQVLAKPASVTPHTRFKAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVAMDVSLIVPIAMEEKLRFAIREGGRTVGAGIVSKIIE

Sequences:

>Translated_391_residues
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEERARGITISTAHVEYETEKRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSKYDFPG
DDIPIVKGSALAALEDKDKSIGEDAVRLLMSEVDNYIPTPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGVIKVGEEV
EIIGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGIERGQVLAKPASVTPHTRFKAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVAMDVSLIVPIAMEEKLRFAIREGGRTVGAGIVSKIIE
>Mature_390_residues
AKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEERARGITISTAHVEYETEKRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSKYDFPGD
DIPIVKGSALAALEDKDKSIGEDAVRLLMSEVDNYIPTPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGVIKVGEEVE
IIGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGIERGQVLAKPASVTPHTRFKAEAYILTKDEGGRHTPFF
TNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVAMDVSLIVPIAMEEKLRFAIREGGRTVGAGIVSKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=54.8223350253807, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=30.1624129930394, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=30.1624129930394, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.125, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=27.4553571428571, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI194018522, Length=347, Percent_Identity=32.2766570605187, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=32.2485207100592, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=32.2485207100592, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=31.6568047337278, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=27.8481012658228, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=26.7657992565056, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI157426893, Length=251, Percent_Identity=27.0916334661355, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=75.1898734177215, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=75.1898734177215, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=31.5508021390374, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=34.8739495798319, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=264, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788922, Length=130, Percent_Identity=32.3076923076923, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.25, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.7777777777778, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.7777777777778, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=27.2093023255814, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=26.8888888888889, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=27.7904328018223, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.7522935779816, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=261, Percent_Identity=31.4176245210728, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=35.5421686746988, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=268, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=26.8398268398268, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.8291457286432, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=29.6380090497738, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=29.6380090497738, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=27.5568181818182, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=29.9120234604106, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.1698113207547, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.4590163934426, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=31.2977099236641, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.0152671755725, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.0152671755725, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.4455958549223, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=28.4444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=28.4444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=28.4444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=28.4444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.5077951002227, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.5077951002227, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.673590504451, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647046, Length=276, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=276, Percent_Identity=26.0869565217391, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=132, Percent_Identity=34.8484848484849, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28572034, Length=159, Percent_Identity=35.8490566037736, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42867; Mature: 42735

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEERARGITIST
CCCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCEEEEE
AHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSKYDFPGDDIPIVKGSALAALEDKDKS
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCC
IGEDAVRLLMSEVDNYIPTPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGVIKVGEEV
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEECCCEE
EIIGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGIERGQVLAKPASVTPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCEEECCCCCCCCC
RFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVAMDVSL
CEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVSKIIE
EEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEERARGITIST
CCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCEEEEE
AHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSKYDFPGDDIPIVKGSALAALEDKDKS
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCC
IGEDAVRLLMSEVDNYIPTPERPVDQPFLMPIEDVFSISGRGTVVTGRVERGVIKVGEEV
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEECCCEE
EIIGIRPTSKTTVTGVEMFRKLLDQGQAGDNIGALLRGIDREGIERGQVLAKPASVTPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCEEECCCCCCCCC
RFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVAMDVSL
CEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVSKIIE
EEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA