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The map label for this gene is nuoB [H]

Identifier: 49475657

GI number: 49475657

Start: 1010247

End: 1010828

Strand: Reverse

Name: nuoB [H]

Synonym: BH08940

Alternate gene names: 49475657

Gene position: 1010828-1010247 (Counterclockwise)

Preceding gene: 49475658

Following gene: 49475656

Centisome position: 52.35

GC content: 40.89

Gene sequence:

>582_bases
ATGGGATTAATATCTAGTAATTCAACAATTACTGCTCCAAAATCAAAAGGAATTATTGATCCTAATACGGGTGAGTTGAT
AGGTTCTGATGATAGATTTTTCTGTGAGGTTAATGCTGAATTATCGGAGAAAGGCTTTTTAGTTACCTCAGCAGATGCTT
TGATTACTTGGGCGCGTACTGGTTCTCTCATGTGGATGAGTTTTGGTTTAGCTTGTTGTGCTATCGAAATGATGCAATGT
TCAATGCCTCATTATGATAATGAGCGTTTTGGATATGCACCACGCGCTTCACCCCGTCAGTCAGACGTTATGGTAGTGGC
AGGTACTCTAACCAATAAAATGGCACCTGCTTTAAGGAAGGTTTATGATCAAATGCCTGAGCCGCGTTATGTGATTTCTA
TGGGATCATGTGCAAATGGTGGCGGGTATTATCATTATTCTTATTCTGTTGTTCGTGGGTGTGACCGTATTGTGCCTGTG
GATATTTATGTTCCAGGATGTCCTCCTACAGCAGAGGCATTACTATATGGTATATTGTTGTTGCAGAAAAAAATCCGTCG
TACCGGATCGATAGAGCGATAG

Upstream 100 bases:

>100_bases
CATTTGGTTCGATAGGTATGTTCGGTTTTTGGTCAATGATTATATTCTTAGCACTTTTAACTGTCGGATTTATATATGAA
TGGAAAAAAGGAGCTCTTGA

Downstream 100 bases:

>100_bases
GGAGCTTTTGTTGTTGATGAGTGAATCATTAGAAGAGCTTGCTGCCTATTTGAAAAGCAAGCTAGGAGATAAGCTTGAAG
AAACTGTTCTTGCATTTGGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MGLISSNSTITAPKSKGIIDPNTGELIGSDDRFFCEVNAELSEKGFLVTSADALITWARTGSLMWMSFGLACCAIEMMQC
SMPHYDNERFGYAPRASPRQSDVMVVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPV
DIYVPGCPPTAEALLYGILLLQKKIRRTGSIER

Sequences:

>Translated_193_residues
MGLISSNSTITAPKSKGIIDPNTGELIGSDDRFFCEVNAELSEKGFLVTSADALITWARTGSLMWMSFGLACCAIEMMQC
SMPHYDNERFGYAPRASPRQSDVMVVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPV
DIYVPGCPPTAEALLYGILLLQKKIRRTGSIER
>Mature_192_residues
GLISSNSTITAPKSKGIIDPNTGELIGSDDRFFCEVNAELSEKGFLVTSADALITWARTGSLMWMSFGLACCAIEMMQCS
MPHYDNERFGYAPRASPRQSDVMVVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVD
IYVPGCPPTAEALLYGILLLQKKIRRTGSIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=151, Percent_Identity=74.8344370860927, Blast_Score=243, Evalue=9e-65,
Organism=Escherichia coli, GI1788624, Length=175, Percent_Identity=44, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789074, Length=125, Percent_Identity=44.8, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=42.6229508196721, Blast_Score=100, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=146, Percent_Identity=65.7534246575342, Blast_Score=217, Evalue=4e-57,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=73.7588652482269, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=73.7588652482269, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=74.468085106383, Blast_Score=231, Evalue=2e-61,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21171; Mature: 21040

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLISSNSTITAPKSKGIIDPNTGELIGSDDRFFCEVNAELSEKGFLVTSADALITWART
CCEECCCCEEECCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCEEEECHHEEEEECC
GSLMWMSFGLACCAIEMMQCSMPHYDNERFGYAPRASPRQSDVMVVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGSIER
HHHHHHHCCCCCC
>Mature Secondary Structure 
GLISSNSTITAPKSKGIIDPNTGELIGSDDRFFCEVNAELSEKGFLVTSADALITWART
CEECCCCEEECCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCEEEECHHEEEEECC
GSLMWMSFGLACCAIEMMQCSMPHYDNERFGYAPRASPRQSDVMVVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGSIER
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA