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The map label for this gene is amiB [H]

Identifier: 49475634

GI number: 49475634

Start: 977816

End: 979045

Strand: Reverse

Name: amiB [H]

Synonym: BH08710

Alternate gene names: 49475634

Gene position: 979045-977816 (Counterclockwise)

Preceding gene: 49475636

Following gene: 49475633

Centisome position: 50.7

GC content: 35.04

Gene sequence:

>1230_bases
ATGATAAGAGGTTTTTCTATAAAAAAGCCAAAAGCAGCTTATTGGCGTATTATTTTACACCTCACATACTGTATATTATG
TTTTTCAATATTCCAAACAAATATCCAAGCTGCGGACGCCCTGGAACTTGTCAATTTGCGAACCATTGGTGATAGTAAGC
GCACGCGTATTATTGCAGTTTTTAATGCTGAACCAAATGTTTTTTTGCAGATTTTTGATAATCCTGCACGTTTGGTTATC
AATTTACCTATTGTTGATTTTTCAGTGCAAAACACATCTTTAAAGAAACAGAATAAATTATCGAGTATGCTCTCAGATGT
GCGTTATAGTTTTTCTGATGTAAAAACTTCACGTATTATTTTGATAAGCAAAACAGCGTTTGTTGTTGAAAAAAGTACAG
TACAAAAATTAGAGAATGGTTCATGGCAACTTTTGATTGATATTACCCAAAGCACACAAAAAAAGTTTGATGAGGTATTG
AAAAAACAACAAAAAGCTGATTTCAATACGAAAATACAGAGAGATCTCCCCCGTAGTTTTCGTGTTGTACTTGATCCTGG
ACATGGTGGTATTGACGGTGGTGCACGGGGAGTTACTGGAATTTTAGAAAAAGATGTAACACTAGCCTTTGCACGTGCAT
TGCGAGATGAATTACAAAAAGGTTCCCACACCATTGTTGCGTTAACACGGGATTCTGATATCTTTCTAAGATTAAGCGAA
AGAGTTAAAAAGGCACAGGAATTTGATGCTGATCTTTTTATATCTATTCATGCCGATACAATTGATGTACATTCTCTACG
TGGTGCTACAGTATATACCATATCGGACGAGGCATCTGATGCAATTGCGAAGTCTTTAGCTGAAAGTGAAAATAAGGTTG
ATCTACTTGACGGTTTACCGAAAGAAGAATCGCTTGAATTAACAGATATTTTGCTCGATCTGACCCGACGTGAAACGCAT
GCATTTTCGATTAATTTTGCTAATAATGTTGTCTCAAATTTATCAAAGAGTCACATCAATTTGATAAATAATCCTCATCG
ATATGCTGATTTTCAAGTTTTAAAAGCTCCAGATGTTCCCTCTGTCTTGATAGAGATAGGTTATTTATCAAATAAAGAGG
ATGAAAAATTATTAAATAATCCCCAATGGAGAAAACAAATGGCTGCTTCTATCGCTTATTCTATTCGTCAATTCGCTGAG
TATAGACAGAAAATTATGCAGCCTCTTTAA

Upstream 100 bases:

>100_bases
TTAATTTAGTTTTTTAGTGTTTAAATTTAAAGCTATTGGCATCACTGTTGTTATAACTAAGATGAATTGTTTGTTTAATG
TAGAGTAGAGTATTTTGCTT

Downstream 100 bases:

>100_bases
ATGGGGGGGGAAATAGAGTATTAATATAATTTTATAGTATAAATATAGAGATGAAAAACAGTTTTTTCTCGCAGTAGTAT
TTCATGTCGCCTTTTTGTTG

Product: N-acetylmuramoyl-l-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MIRGFSIKKPKAAYWRIILHLTYCILCFSIFQTNIQAADALELVNLRTIGDSKRTRIIAVFNAEPNVFLQIFDNPARLVI
NLPIVDFSVQNTSLKKQNKLSSMLSDVRYSFSDVKTSRIILISKTAFVVEKSTVQKLENGSWQLLIDITQSTQKKFDEVL
KKQQKADFNTKIQRDLPRSFRVVLDPGHGGIDGGARGVTGILEKDVTLAFARALRDELQKGSHTIVALTRDSDIFLRLSE
RVKKAQEFDADLFISIHADTIDVHSLRGATVYTISDEASDAIAKSLAESENKVDLLDGLPKEESLELTDILLDLTRRETH
AFSINFANNVVSNLSKSHINLINNPHRYADFQVLKAPDVPSVLIEIGYLSNKEDEKLLNNPQWRKQMAASIAYSIRQFAE
YRQKIMQPL

Sequences:

>Translated_409_residues
MIRGFSIKKPKAAYWRIILHLTYCILCFSIFQTNIQAADALELVNLRTIGDSKRTRIIAVFNAEPNVFLQIFDNPARLVI
NLPIVDFSVQNTSLKKQNKLSSMLSDVRYSFSDVKTSRIILISKTAFVVEKSTVQKLENGSWQLLIDITQSTQKKFDEVL
KKQQKADFNTKIQRDLPRSFRVVLDPGHGGIDGGARGVTGILEKDVTLAFARALRDELQKGSHTIVALTRDSDIFLRLSE
RVKKAQEFDADLFISIHADTIDVHSLRGATVYTISDEASDAIAKSLAESENKVDLLDGLPKEESLELTDILLDLTRRETH
AFSINFANNVVSNLSKSHINLINNPHRYADFQVLKAPDVPSVLIEIGYLSNKEDEKLLNNPQWRKQMAASIAYSIRQFAE
YRQKIMQPL
>Mature_409_residues
MIRGFSIKKPKAAYWRIILHLTYCILCFSIFQTNIQAADALELVNLRTIGDSKRTRIIAVFNAEPNVFLQIFDNPARLVI
NLPIVDFSVQNTSLKKQNKLSSMLSDVRYSFSDVKTSRIILISKTAFVVEKSTVQKLENGSWQLLIDITQSTQKKFDEVL
KKQQKADFNTKIQRDLPRSFRVVLDPGHGGIDGGARGVTGILEKDVTLAFARALRDELQKGSHTIVALTRDSDIFLRLSE
RVKKAQEFDADLFISIHADTIDVHSLRGATVYTISDEASDAIAKSLAESENKVDLLDGLPKEESLELTDILLDLTRRETH
AFSINFANNVVSNLSKSHINLINNPHRYADFQVLKAPDVPSVLIEIGYLSNKEDEKLLNNPQWRKQMAASIAYSIRQFAE
YRQKIMQPL

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=401, Percent_Identity=29.426433915212, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1788776, Length=217, Percent_Identity=40.5529953917051, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=33.0396475770925, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46235; Mature: 46235

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRGFSIKKPKAAYWRIILHLTYCILCFSIFQTNIQAADALELVNLRTIGDSKRTRIIAV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCEEEEEE
FNAEPNVFLQIFDNPARLVINLPIVDFSVQNTSLKKQNKLSSMLSDVRYSFSDVKTSRII
EECCCCEEEEEECCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LISKTAFVVEKSTVQKLENGSWQLLIDITQSTQKKFDEVLKKQQKADFNTKIQRDLPRSF
EEECCCCEEEHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEE
RVVLDPGHGGIDGGARGVTGILEKDVTLAFARALRDELQKGSHTIVALTRDSDIFLRLSE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHH
RVKKAQEFDADLFISIHADTIDVHSLRGATVYTISDEASDAIAKSLAESENKVDLLDGLP
HHHHHHHCCCEEEEEEECCEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEHHCCCC
KEESLELTDILLDLTRRETHAFSINFANNVVSNLSKSHINLINNPHRYADFQVLKAPDVP
CCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
SVLIEIGYLSNKEDEKLLNNPQWRKQMAASIAYSIRQFAEYRQKIMQPL
EEHEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRGFSIKKPKAAYWRIILHLTYCILCFSIFQTNIQAADALELVNLRTIGDSKRTRIIAV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCEEEEEE
FNAEPNVFLQIFDNPARLVINLPIVDFSVQNTSLKKQNKLSSMLSDVRYSFSDVKTSRII
EECCCCEEEEEECCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LISKTAFVVEKSTVQKLENGSWQLLIDITQSTQKKFDEVLKKQQKADFNTKIQRDLPRSF
EEECCCCEEEHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEE
RVVLDPGHGGIDGGARGVTGILEKDVTLAFARALRDELQKGSHTIVALTRDSDIFLRLSE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHH
RVKKAQEFDADLFISIHADTIDVHSLRGATVYTISDEASDAIAKSLAESENKVDLLDGLP
HHHHHHHCCCEEEEEEECCEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEHHCCCC
KEESLELTDILLDLTRRETHAFSINFANNVVSNLSKSHINLINNPHRYADFQVLKAPDVP
CCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
SVLIEIGYLSNKEDEKLLNNPQWRKQMAASIAYSIRQFAEYRQKIMQPL
EEHEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]