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The map label for this gene is livM [H]

Identifier: 49475592

GI number: 49475592

Start: 932999

End: 934057

Strand: Reverse

Name: livM [H]

Synonym: BH08270

Alternate gene names: 49475592

Gene position: 934057-932999 (Counterclockwise)

Preceding gene: 49475593

Following gene: 49475591

Centisome position: 48.37

GC content: 37.39

Gene sequence:

>1059_bases
ATGGCAAAATCAGCTTCAACTTTTTTATCATGTATCAGTATTCTTTTTTTTATCGGTTTTTTATTTTATGCTGATAACCA
TTTTAATGAGTACACACTTCGTGTCATCAACCTTATTGCTATCAATGCGATATTGGCAATTTCGCTTAATTTGATTTACG
GTTTCACCGGGATGTTTTCTCTTGGACATGCTGGATTCATGGCTATTGGTGCTTATGTATGCGCGATTTTACTTCTTTCT
CCTGAACAAAAAGAAATGATGTGGATTCTTGAACCTATAGCTGAGCCATTGCATGATCTACAGTTACCTTTTTTTGTAAC
TGTTGTTGTAAGTGGTCTGTGTGCTGCAGTTGTAGGTTTATTAATTGCTCTTCCTATCTTACGTCTTGGCGGAGATTATC
TTGGAATTGCAACGTTGGGTTTTGCAGAAATTATTCGCATTGTTATTACAAACGCGACATCCGTAACAAATGGTTCTTTA
GGAATCAAAGGAATACCTCAAAATGCGACATTATGGTGGAACTATAGTTGGTTAGTATTTACGGTTACCTTCATTATTCT
CTTACTGCGAAGCAATACTGGTAATGTACTGCGCGCTATTCGTGATGATGAAATCGCTGCGAAAACAATGGGGATTAACG
CTTTTTTCTACCGTAGCTTTTCCTTTACTGCAGGTGCATTCTTTGCAGGTGTCGGAGGAGCATTAATGGCCGCTCTTATT
TCAACAATCGATCCAAAAATGTTCAACTTCCTTCTAACTTTTAATATTTTGATGATTGTTGTTGCTGGTGGTCTTGGTTC
GATTACAGGTAGCATTATTGGCAGCATTATTATTACGGTCATGCTTGAATGGCTTCGCATTATCGAGAGTCCATTTGACT
TAGGTTTTATACATATTCCAGAGACACCAGGGCTTAGGATGGTTATTTTTTCTCTTTTATTGCTCGTCATTATCTTATTT
TGGAGAAGAGGTTTATTGGGACAACGTGAATTCTCATGGAGCGGGATTTATAGCTTTTTTACACGCAAAAAGTTCTCTAA
TACTGAGGAAAAGCTATGA

Upstream 100 bases:

>100_bases
TATCCGGATATAGAGATGCTTTTGCCTTTATTTTATTAATTCTGATTTTATTAGTAATGCCCACTGGTTTAATGGGCAAA
AAAAATCAGGAGAAAATCTA

Downstream 100 bases:

>100_bases
ACGACACTATTCTTTTACTCAATGATATCGTGATGCGTTTTGGCGGATTGGTTGCTGTCAATAATATTAATATGGCAATC
AAGCGAGGAAAAATTACTGG

Product: high-affinity branched-chain amino acid transporter, permease protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAKSASTFLSCISILFFIGFLFYADNHFNEYTLRVINLIAINAILAISLNLIYGFTGMFSLGHAGFMAIGAYVCAILLLS
PEQKEMMWILEPIAEPLHDLQLPFFVTVVVSGLCAAVVGLLIALPILRLGGDYLGIATLGFAEIIRIVITNATSVTNGSL
GIKGIPQNATLWWNYSWLVFTVTFIILLLRSNTGNVLRAIRDDEIAAKTMGINAFFYRSFSFTAGAFFAGVGGALMAALI
STIDPKMFNFLLTFNILMIVVAGGLGSITGSIIGSIIITVMLEWLRIIESPFDLGFIHIPETPGLRMVIFSLLLLVIILF
WRRGLLGQREFSWSGIYSFFTRKKFSNTEEKL

Sequences:

>Translated_352_residues
MAKSASTFLSCISILFFIGFLFYADNHFNEYTLRVINLIAINAILAISLNLIYGFTGMFSLGHAGFMAIGAYVCAILLLS
PEQKEMMWILEPIAEPLHDLQLPFFVTVVVSGLCAAVVGLLIALPILRLGGDYLGIATLGFAEIIRIVITNATSVTNGSL
GIKGIPQNATLWWNYSWLVFTVTFIILLLRSNTGNVLRAIRDDEIAAKTMGINAFFYRSFSFTAGAFFAGVGGALMAALI
STIDPKMFNFLLTFNILMIVVAGGLGSITGSIIGSIIITVMLEWLRIIESPFDLGFIHIPETPGLRMVIFSLLLLVIILF
WRRGLLGQREFSWSGIYSFFTRKKFSNTEEKL
>Mature_351_residues
AKSASTFLSCISILFFIGFLFYADNHFNEYTLRVINLIAINAILAISLNLIYGFTGMFSLGHAGFMAIGAYVCAILLLSP
EQKEMMWILEPIAEPLHDLQLPFFVTVVVSGLCAAVVGLLIALPILRLGGDYLGIATLGFAEIIRIVITNATSVTNGSLG
IKGIPQNATLWWNYSWLVFTVTFIILLLRSNTGNVLRAIRDDEIAAKTMGINAFFYRSFSFTAGAFFAGVGGALMAALIS
TIDPKMFNFLLTFNILMIVVAGGLGSITGSIIGSIIITVMLEWLRIIESPFDLGFIHIPETPGLRMVIFSLLLLVIILFW
RRGLLGQREFSWSGIYSFFTRKKFSNTEEKL

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=289, Percent_Identity=33.2179930795848, Blast_Score=131, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38790; Mature: 38659

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSASTFLSCISILFFIGFLFYADNHFNEYTLRVINLIAINAILAISLNLIYGFTGMFS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHAGFMAIGAYVCAILLLSPEQKEMMWILEPIAEPLHDLQLPFFVTVVVSGLCAAVVGL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIALPILRLGGDYLGIATLGFAEIIRIVITNATSVTNGSLGIKGIPQNATLWWNYSWLVF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHH
TVTFIILLLRSNTGNVLRAIRDDEIAAKTMGINAFFYRSFSFTAGAFFAGVGGALMAALI
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHHH
STIDPKMFNFLLTFNILMIVVAGGLGSITGSIIGSIIITVMLEWLRIIESPFDLGFIHIP
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
ETPGLRMVIFSLLLLVIILFWRRGLLGQREFSWSGIYSFFTRKKFSNTEEKL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKSASTFLSCISILFFIGFLFYADNHFNEYTLRVINLIAINAILAISLNLIYGFTGMFS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHAGFMAIGAYVCAILLLSPEQKEMMWILEPIAEPLHDLQLPFFVTVVVSGLCAAVVGL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIALPILRLGGDYLGIATLGFAEIIRIVITNATSVTNGSLGIKGIPQNATLWWNYSWLVF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHH
TVTFIILLLRSNTGNVLRAIRDDEIAAKTMGINAFFYRSFSFTAGAFFAGVGGALMAALI
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHHH
STIDPKMFNFLLTFNILMIVVAGGLGSITGSIIGSIIITVMLEWLRIIESPFDLGFIHIP
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
ETPGLRMVIFSLLLLVIILFWRRGLLGQREFSWSGIYSFFTRKKFSNTEEKL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]