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The map label for this gene is dme [H]

Identifier: 49475518

GI number: 49475518

Start: 854064

End: 856388

Strand: Reverse

Name: dme [H]

Synonym: BH07470

Alternate gene names: 49475518

Gene position: 856388-854064 (Counterclockwise)

Preceding gene: 49475519

Following gene: 49475517

Centisome position: 44.35

GC content: 39.7

Gene sequence:

>2325_bases
ATGTCTACAGAACGAAATAATGATTTCAGTTCACAAAAAACTGAACTTGACAGTGCCGCACTTTTTTATCATCAACATCC
AAAGCCTGGAAAATTAGAAATCCAAGCTACAACACCTCTTGATAATCAACGTGATTTAGCTCTTGCTTATTCTCCAGGTG
TTGCTGCACCATGTCTTGCAATTCATAAAGATCCAAATCTTGCTGCACAATATACTTCTCGTTCTAATTTAGTTGCCGTT
ATATCAAATGGAACTGCTGTTCTTGGATTAGGAAATATTGGTCCCCTCGCCTCAAAACCGGTTATGGAAGGAAAAGCCGT
TTTATTCAAAAAATTTGCGAATATCGATGTTTTTGATATTGAAATTGATGCATCTGATATTGAACAAATGGTAAAAACCG
TATCTGCCTTAGAACCTACATTTGGTGGTATAAATCTTGAAGATATTAAGGCTCCTGAATGTTTCGAAATCGAAGAAAAA
CTTCGCACTAAGATGAATATTCCAGTTTTCCATGATGACCAACATGGAACTGCTATTATTGTTTCTGCAGCGGTATTAAA
TGCATTAAGTCTTGCAGGAAAAAAAATTGAAAATGTAAAAATAGTTTCTTCAGGCGCGGGGGCAGCAGCTCTCGCTTGTA
TCAACCTTTTGGTCCGTCTTGGAGCAAAAGTTGAAAATATTTGGCTTAGTGACTTAGAGGGGGTTGTTTATGAAGGTCGC
AAGACACTTATGGATCGTTGGAAAATCAGTTATGCACAAAAAACTGATGCACGCACTTTATCTGAAATTATTGATGATGC
AGATATCTTTTTAGGTGTTTCAGCAGGTGGCGTTTTAAAACCTGAATATCTGAAAAAAATGGCTCAAAATCCCTTAATTT
TGGCCCTTGCTAATCCAACACCAGAAATTATGCCAGAAGAAGCACGTTCTGTGCGACCAGATGCAATGATCTGCACAGGA
CGCTCTGATTATCCCAATCAAGTAAACAATGTCCTTTGTTTTCCTTATATCTTCCGTGGTGCATTAGATGTTGGTGCAAC
CGCAATCAATGAAGAAATGAAAATAGCCGCAGTTCATGCAATTGCTGCCCTCGCCCGCGAAGAAACTTCAGATATTGCAG
CACGTGCATATTCAAAGAAACCACCTAGTTTTGGTCCAGATTATCTTATTCCCTCTCCCTTCGATCCACGCTTAATACTA
CGCATTGCTCCTGCCGTGGCTAAAGCAGCAATGGAAACAGAGGTAGCGATTCGGCCAATTGAAGATATGGAAGCTTATCA
TGATATCCTTAACCGATTCGTATTTCGTTCTGGATTAACAATGAAGCCCGTTTTTGCAGCAGCAAAAACAGCAAAACGTA
AACGCGTCATTTATGCCAATGGTGAAGATGAACGCGTTCTTCGAGCAGCGCAAATTGTGCTTGAAGAACAAACAGCAATT
CCACTCCTCATCGGCCGTCCTCATGTAGTGGAGACGAGACTAAAACGCTTTGGTTTAAAAATTCGCCCTGGCATAGATTT
TGAACTTACAAATCCAGAAGATGATCCACGTTTTCGTGATTATGTTAGCTTATTTCTCCGTTATACAGGAAGACGTGGTG
TTTCACCCGAAGTTGCAAAAACAATCGTGAGAACCTCAACAACAGCGATTGCTGCCCTCGCAGTTATGCGTGAAGAAGCT
GATGCAATGATTTGCGGTTTAGAAGGACGTTTTGAACGTCACCTTGAATTAATTGAACAAGTTATTGGACTTGATTCAAA
TACTCGTCATTTTTCTGCTGTGAGTTTGCTTATTTCTCCACGCCGCACCCTTTTTTTAACAGATACTTATGTTAATGAAG
ATCCTTCTGCAGAAGAAATAGCAGAAATGACAGTACTGGCTGCAAAAGAAATTGAAGCATTTGGTATAACACCCAAAGCA
GCATTATTATCACACTCAAACTTTGGCTCCAAAAATACAGAAAGTGCGCGTAAAATGCGCCGTGCAACTGAAATTCTTGC
TCAATTATACCCAAATTTAGAAGCCGATGGAGAAATGCACGGAGATGCTGCTCTTTCTAAAGTTTTTCGTGATCGTGTTT
TTCCTGATTCACGTCTTAAAGGTGAAGCAAATTTGCTTGTCTTCCCAACACTTGATGCCGCTAATATAACACTCAATCTC
GTAAAAAATCTCACCAATGCACTTCATGTAGGACCTATTTTAATCGGAGCCTCACGTCCTGCACACATCTTAACACCTTC
AGTAACTTCACGAGGAGTGGTTAATATGACAGCTCTTGCTGTGCTTGCTGCAAATAGAAAAAAATTGCATAATGAAAATA
AATAA

Upstream 100 bases:

>100_bases
AGATTTTTGTATTCAGAGAAATTAGAATCTATCAGTGTACAAGGCCTTATGGTAGATGGATCCCCATATATAAACATATA
CTAACCTGGATATCATAAAA

Downstream 100 bases:

>100_bases
AGTCACAATAAAACATCAATTTTTTTCAAAACCTTGATGTATGATATTTCATGTCCTATAAACTTATTTTCCATAGTTAA
GTCTTTTGTTAAAAACTAAA

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MSTERNNDFSSQKTELDSAALFYHQHPKPGKLEIQATTPLDNQRDLALAYSPGVAAPCLAIHKDPNLAAQYTSRSNLVAV
ISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFANIDVFDIEIDASDIEQMVKTVSALEPTFGGINLEDIKAPECFEIEEK
LRTKMNIPVFHDDQHGTAIIVSAAVLNALSLAGKKIENVKIVSSGAGAAALACINLLVRLGAKVENIWLSDLEGVVYEGR
KTLMDRWKISYAQKTDARTLSEIIDDADIFLGVSAGGVLKPEYLKKMAQNPLILALANPTPEIMPEEARSVRPDAMICTG
RSDYPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVHAIAALAREETSDIAARAYSKKPPSFGPDYLIPSPFDPRLIL
RIAPAVAKAAMETEVAIRPIEDMEAYHDILNRFVFRSGLTMKPVFAAAKTAKRKRVIYANGEDERVLRAAQIVLEEQTAI
PLLIGRPHVVETRLKRFGLKIRPGIDFELTNPEDDPRFRDYVSLFLRYTGRRGVSPEVAKTIVRTSTTAIAALAVMREEA
DAMICGLEGRFERHLELIEQVIGLDSNTRHFSAVSLLISPRRTLFLTDTYVNEDPSAEEIAEMTVLAAKEIEAFGITPKA
ALLSHSNFGSKNTESARKMRRATEILAQLYPNLEADGEMHGDAALSKVFRDRVFPDSRLKGEANLLVFPTLDAANITLNL
VKNLTNALHVGPILIGASRPAHILTPSVTSRGVVNMTALAVLAANRKKLHNENK

Sequences:

>Translated_774_residues
MSTERNNDFSSQKTELDSAALFYHQHPKPGKLEIQATTPLDNQRDLALAYSPGVAAPCLAIHKDPNLAAQYTSRSNLVAV
ISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFANIDVFDIEIDASDIEQMVKTVSALEPTFGGINLEDIKAPECFEIEEK
LRTKMNIPVFHDDQHGTAIIVSAAVLNALSLAGKKIENVKIVSSGAGAAALACINLLVRLGAKVENIWLSDLEGVVYEGR
KTLMDRWKISYAQKTDARTLSEIIDDADIFLGVSAGGVLKPEYLKKMAQNPLILALANPTPEIMPEEARSVRPDAMICTG
RSDYPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVHAIAALAREETSDIAARAYSKKPPSFGPDYLIPSPFDPRLIL
RIAPAVAKAAMETEVAIRPIEDMEAYHDILNRFVFRSGLTMKPVFAAAKTAKRKRVIYANGEDERVLRAAQIVLEEQTAI
PLLIGRPHVVETRLKRFGLKIRPGIDFELTNPEDDPRFRDYVSLFLRYTGRRGVSPEVAKTIVRTSTTAIAALAVMREEA
DAMICGLEGRFERHLELIEQVIGLDSNTRHFSAVSLLISPRRTLFLTDTYVNEDPSAEEIAEMTVLAAKEIEAFGITPKA
ALLSHSNFGSKNTESARKMRRATEILAQLYPNLEADGEMHGDAALSKVFRDRVFPDSRLKGEANLLVFPTLDAANITLNL
VKNLTNALHVGPILIGASRPAHILTPSVTSRGVVNMTALAVLAANRKKLHNENK
>Mature_773_residues
STERNNDFSSQKTELDSAALFYHQHPKPGKLEIQATTPLDNQRDLALAYSPGVAAPCLAIHKDPNLAAQYTSRSNLVAVI
SNGTAVLGLGNIGPLASKPVMEGKAVLFKKFANIDVFDIEIDASDIEQMVKTVSALEPTFGGINLEDIKAPECFEIEEKL
RTKMNIPVFHDDQHGTAIIVSAAVLNALSLAGKKIENVKIVSSGAGAAALACINLLVRLGAKVENIWLSDLEGVVYEGRK
TLMDRWKISYAQKTDARTLSEIIDDADIFLGVSAGGVLKPEYLKKMAQNPLILALANPTPEIMPEEARSVRPDAMICTGR
SDYPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVHAIAALAREETSDIAARAYSKKPPSFGPDYLIPSPFDPRLILR
IAPAVAKAAMETEVAIRPIEDMEAYHDILNRFVFRSGLTMKPVFAAAKTAKRKRVIYANGEDERVLRAAQIVLEEQTAIP
LLIGRPHVVETRLKRFGLKIRPGIDFELTNPEDDPRFRDYVSLFLRYTGRRGVSPEVAKTIVRTSTTAIAALAVMREEAD
AMICGLEGRFERHLELIEQVIGLDSNTRHFSAVSLLISPRRTLFLTDTYVNEDPSAEEIAEMTVLAAKEIEAFGITPKAA
LLSHSNFGSKNTESARKMRRATEILAQLYPNLEADGEMHGDAALSKVFRDRVFPDSRLKGEANLLVFPTLDAANITLNLV
KNLTNALHVGPILIGASRPAHILTPSVTSRGVVNMTALAVLAANRKKLHNENK

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=430, Percent_Identity=22.5581395348837, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI62420882, Length=436, Percent_Identity=23.8532110091743, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI62420880, Length=436, Percent_Identity=23.8532110091743, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI239049447, Length=436, Percent_Identity=23.8532110091743, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI4505145, Length=353, Percent_Identity=25.2124645892351, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=26.5625, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788806, Length=757, Percent_Identity=57.0673712021136, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=316, Percent_Identity=25.9493670886076, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788800, Length=315, Percent_Identity=27.9365079365079, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537199, Length=424, Percent_Identity=21.9339622641509, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=270, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362672, Length=410, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281362674, Length=410, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356279, Length=410, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24646388, Length=350, Percent_Identity=26.8571428571429, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=26.8571428571429, Blast_Score=81, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281363505, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707236, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707232, Length=351, Percent_Identity=26.2108262108262, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363503, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707242, Length=352, Percent_Identity=23.8636363636364, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI78707238, Length=352, Percent_Identity=23.8636363636364, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI78707240, Length=352, Percent_Identity=23.8636363636364, Blast_Score=73, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 84651; Mature: 84519

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTERNNDFSSQKTELDSAALFYHQHPKPGKLEIQATTPLDNQRDLALAYSPGVAAPCLA
CCCCCCCCCCHHHHHCCHHEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEE
IHKDPNLAAQYTSRSNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFANIDVFDI
EECCCCCEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
EIDASDIEQMVKTVSALEPTFGGINLEDIKAPECFEIEEKLRTKMNIPVFHDDQHGTAII
EECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEE
VSAAVLNALSLAGKKIENVKIVSSGAGAAALACINLLVRLGAKVENIWLSDLEGVVYEGR
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KTLMDRWKISYAQKTDARTLSEIIDDADIFLGVSAGGVLKPEYLKKMAQNPLILALANPT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCC
PEIMPEEARSVRPDAMICTGRSDYPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVHA
CCCCCCHHHCCCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHCCCHHHHHHHH
IAALAREETSDIAARAYSKKPPSFGPDYLIPSPFDPRLILRIAPAVAKAAMETEVAIRPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHEECCH
EDMEAYHDILNRFVFRSGLTMKPVFAAAKTAKRKRVIYANGEDERVLRAAQIVLEEQTAI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCC
PLLIGRPHVVETRLKRFGLKIRPGIDFELTNPEDDPRFRDYVSLFLRYTGRRGVSPEVAK
EEEECCCHHHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
TIVRTSTTAIAALAVMREEADAMICGLEGRFERHLELIEQVIGLDSNTRHFSAVSLLISP
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECC
RRTLFLTDTYVNEDPSAEEIAEMTVLAAKEIEAFGITPKAALLSHSNFGSKNTESARKMR
CEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
RATEILAQLYPNLEADGEMHGDAALSKVFRDRVFPDSRLKGEANLLVFPTLDAANITLNL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHH
VKNLTNALHVGPILIGASRPAHILTPSVTSRGVVNMTALAVLAANRKKLHNENK
HHHHHHHHHHCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STERNNDFSSQKTELDSAALFYHQHPKPGKLEIQATTPLDNQRDLALAYSPGVAAPCLA
CCCCCCCCCHHHHHCCHHEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEE
IHKDPNLAAQYTSRSNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFANIDVFDI
EECCCCCEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
EIDASDIEQMVKTVSALEPTFGGINLEDIKAPECFEIEEKLRTKMNIPVFHDDQHGTAII
EECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEE
VSAAVLNALSLAGKKIENVKIVSSGAGAAALACINLLVRLGAKVENIWLSDLEGVVYEGR
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KTLMDRWKISYAQKTDARTLSEIIDDADIFLGVSAGGVLKPEYLKKMAQNPLILALANPT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCC
PEIMPEEARSVRPDAMICTGRSDYPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVHA
CCCCCCHHHCCCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHCCCHHHHHHHH
IAALAREETSDIAARAYSKKPPSFGPDYLIPSPFDPRLILRIAPAVAKAAMETEVAIRPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHEECCH
EDMEAYHDILNRFVFRSGLTMKPVFAAAKTAKRKRVIYANGEDERVLRAAQIVLEEQTAI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCC
PLLIGRPHVVETRLKRFGLKIRPGIDFELTNPEDDPRFRDYVSLFLRYTGRRGVSPEVAK
EEEECCCHHHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
TIVRTSTTAIAALAVMREEADAMICGLEGRFERHLELIEQVIGLDSNTRHFSAVSLLISP
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECC
RRTLFLTDTYVNEDPSAEEIAEMTVLAAKEIEAFGITPKAALLSHSNFGSKNTESARKMR
CEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
RATEILAQLYPNLEADGEMHGDAALSKVFRDRVFPDSRLKGEANLLVFPTLDAANITLNL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHH
VKNLTNALHVGPILIGASRPAHILTPSVTSRGVVNMTALAVLAANRKKLHNENK
HHHHHHHHHHCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]