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The map label for this gene is thiG

Identifier: 49475283

GI number: 49475283

Start: 567383

End: 568156

Strand: Reverse

Name: thiG

Synonym: BH04880

Alternate gene names: 49475283

Gene position: 568156-567383 (Counterclockwise)

Preceding gene: 49475284

Following gene: 49475282

Centisome position: 29.42

GC content: 40.83

Gene sequence:

>774_bases
ATGCTGAATCTGTACGGACATAAATTTTCTTCTCGTCTTATGTTAGGTACAGCGCAATATCCTTCACCAGCAATTCTTCG
TGATGCTATTCATAAATCGAATACAGAAATTGTAACAGTTTCGTTACGCCGAGAAACCGCTGGTGGAAAACAAGGTGGAC
AATTTTGGCAGTTTCTTAAAGAACTGGATATAACAGTGCTCCCCAATACCGCTGGTTGTTATACTGTTAAAGAAGCTGTT
ACAACCGCGAAACTTGCACGAGATCTCTTTAAAACATCTTGGATTAAACTCGAAATTATCGGTAATCCCGATACTCTACA
GCCAAATGTTTTTTCTTTAGTCGAAGCAGCAAAAATTTTAAACAGTGAAGGTTTCAAAATTTTTGCCTATACAACAGATG
ATCTCATTGTTGCTGAAAAGCTCTTGGATGTTGGCTGTCAAGTCATTATGCCTTGGTGTGCTCCTATTGGATCTGCAAAA
GGCCCACATAATACTGATGGATTGCGTTCTATCCGTGCTTACCTTCCTGATGTTACTCTTGTTATTGATGCTGGCATTGG
ACGCCCATCACATGCTACCGTTGCCATGGAACTTGGTTATGATGCAGTGCTTCTTAACACCGCAGTTGCTAAAGCAGCTG
ATCCTGTCTTAATGGCTGAAGCATTTTCTAAAGCTATTCAGGCAGGACGTATGGGATATAAAGCGGGCATTCTTGAAGCA
CGCAATGTAGCTGTTCCTTCAACGCCAATTATTGGAAAAGCAGTATTTTCATGA

Upstream 100 bases:

>100_bases
TGTTAATGCAGAAGTTGTTCCCATAGATGCACGAAGCCGATGTGTCTTACATGAAGGAGATAAAATTGAAATCTTAAGCC
CAATGCAAGGAGGCTAAGCT

Downstream 100 bases:

>100_bases
AACTCGATCCATTTTATCCCATCGTTGACAATGCGGATTGGGTTGAGCGTTTGGTTCCTCTTGGCATCAAACTTATACAA
TTGCGTATGAAAAATGAAAA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLNLYGHKFSSRLMLGTAQYPSPAILRDAIHKSNTEIVTVSLRRETAGGKQGGQFWQFLKELDITVLPNTAGCYTVKEAV
TTAKLARDLFKTSWIKLEIIGNPDTLQPNVFSLVEAAKILNSEGFKIFAYTTDDLIVAEKLLDVGCQVIMPWCAPIGSAK
GPHNTDGLRSIRAYLPDVTLVIDAGIGRPSHATVAMELGYDAVLLNTAVAKAADPVLMAEAFSKAIQAGRMGYKAGILEA
RNVAVPSTPIIGKAVFS

Sequences:

>Translated_257_residues
MLNLYGHKFSSRLMLGTAQYPSPAILRDAIHKSNTEIVTVSLRRETAGGKQGGQFWQFLKELDITVLPNTAGCYTVKEAV
TTAKLARDLFKTSWIKLEIIGNPDTLQPNVFSLVEAAKILNSEGFKIFAYTTDDLIVAEKLLDVGCQVIMPWCAPIGSAK
GPHNTDGLRSIRAYLPDVTLVIDAGIGRPSHATVAMELGYDAVLLNTAVAKAADPVLMAEAFSKAIQAGRMGYKAGILEA
RNVAVPSTPIIGKAVFS
>Mature_257_residues
MLNLYGHKFSSRLMLGTAQYPSPAILRDAIHKSNTEIVTVSLRRETAGGKQGGQFWQFLKELDITVLPNTAGCYTVKEAV
TTAKLARDLFKTSWIKLEIIGNPDTLQPNVFSLVEAAKILNSEGFKIFAYTTDDLIVAEKLLDVGCQVIMPWCAPIGSAK
GPHNTDGLRSIRAYLPDVTLVIDAGIGRPSHATVAMELGYDAVLLNTAVAKAADPVLMAEAFSKAIQAGRMGYKAGILEA
RNVAVPSTPIIGKAVFS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=37.3015873015873, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BARHE (Q6G480)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033324.1
- HSSP:   Q9I6B4
- ProteinModelPortal:   Q6G480
- SMR:   Q6G480
- GeneID:   2865216
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH04880
- NMPDR:   fig|283166.1.peg.452
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q6G480
- ProtClustDB:   PRK00208
- BioCyc:   BHEN283166:BH04880-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27648; Mature: 27648

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLYGHKFSSRLMLGTAQYPSPAILRDAIHKSNTEIVTVSLRRETAGGKQGGQFWQFLK
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHH
ELDITVLPNTAGCYTVKEAVTTAKLARDLFKTSWIKLEIIGNPDTLQPNVFSLVEAAKIL
HCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
NSEGFKIFAYTTDDLIVAEKLLDVGCQVIMPWCAPIGSAKGPHNTDGLRSIRAYLPDVTL
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEE
VIDAGIGRPSHATVAMELGYDAVLLNTAVAKAADPVLMAEAFSKAIQAGRMGYKAGILEA
EEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCC
RNVAVPSTPIIGKAVFS
CCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MLNLYGHKFSSRLMLGTAQYPSPAILRDAIHKSNTEIVTVSLRRETAGGKQGGQFWQFLK
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHH
ELDITVLPNTAGCYTVKEAVTTAKLARDLFKTSWIKLEIIGNPDTLQPNVFSLVEAAKIL
HCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
NSEGFKIFAYTTDDLIVAEKLLDVGCQVIMPWCAPIGSAKGPHNTDGLRSIRAYLPDVTL
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEE
VIDAGIGRPSHATVAMELGYDAVLLNTAVAKAADPVLMAEAFSKAIQAGRMGYKAGILEA
EEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCC
RNVAVPSTPIIGKAVFS
CCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA