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The map label for this gene is yieH [H]

Identifier: 49475249

GI number: 49475249

Start: 520939

End: 521640

Strand: Reverse

Name: yieH [H]

Synonym: BH04520

Alternate gene names: 49475249

Gene position: 521640-520939 (Counterclockwise)

Preceding gene: 49475251

Following gene: 49475248

Centisome position: 27.01

GC content: 36.89

Gene sequence:

>702_bases
ATGCCTAAGATAGATCTCATTATTTTTGATTGTGATGGAGTTCTCGTAGATTCTGAATATCTCGCAGCACAAATTGGATC
TCAATTACTTAAGAAAATTGGATATGAAATATCACCTGAAGAATTGAGTGCGCGTTATGCAGGACTAATCTTTCCTGATA
TTCTTAAAAAAGTTGAACAAGAAACAGGAAAACCAACTTCTGCTCATCTTATAGATCAGATGTCAGATCTTTTTCGAGCA
CAAATAAAAACTGAACTACGTGCTATCGATGGTATAAAAGAAGCGATAGAAGCTATACAATCTCACTATCCTTATTGTAT
TTGCTCTAATGCAAGAAGCACAGAGATAAAAGAAATGCTCACTATCGTTGATCTTTATGATTTTTTTGATAATAAAGATA
AAATATTTTCTGCTCCTGAAGTTGGAACAAAAAAAACGAAACCTGCTCCTGATGTTTTTCTTTTCGCTGCACAACAATTG
CGCGTAAAACCAAAAAACACTATTGTTATAGAAGATTCAATTCATGGCGTACATGCTGCAACCGCAGCAGGCATGCGCGT
TATCGGTTTTACTGGAGGATCGCATAGCTATCTTGGCCACTCTAATGCACTTGTAGAGGCTGGTGCTGAAACAGCTATCG
CAAAACATGCCCATTTACTAGAAGTCTTAGAAGCAATGGCAGTGTGGCAAGATTTACCATAA

Upstream 100 bases:

>100_bases
GGAATCACTTTCAAAGCATATTTTTATGAAATAAAGTCTTCTCAGTGTCATTCTAAAGCAAGTTTTCAAGCTTAATAAGT
AACATGTAAAGGAATATCTT

Downstream 100 bases:

>100_bases
AAAGATCCCTTAGAAATATATCAACTTACACTGTTTTTCAGTAATATTTTTACTAAAATTTTTTTGCCTTGATAAAATAT
ACCACAAGAATTAAGAGATA

Product: phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPKIDLIIFDCDGVLVDSEYLAAQIGSQLLKKIGYEISPEELSARYAGLIFPDILKKVEQETGKPTSAHLIDQMSDLFRA
QIKTELRAIDGIKEAIEAIQSHYPYCICSNARSTEIKEMLTIVDLYDFFDNKDKIFSAPEVGTKKTKPAPDVFLFAAQQL
RVKPKNTIVIEDSIHGVHAATAAGMRVIGFTGGSHSYLGHSNALVEAGAETAIAKHAHLLEVLEAMAVWQDLP

Sequences:

>Translated_233_residues
MPKIDLIIFDCDGVLVDSEYLAAQIGSQLLKKIGYEISPEELSARYAGLIFPDILKKVEQETGKPTSAHLIDQMSDLFRA
QIKTELRAIDGIKEAIEAIQSHYPYCICSNARSTEIKEMLTIVDLYDFFDNKDKIFSAPEVGTKKTKPAPDVFLFAAQQL
RVKPKNTIVIEDSIHGVHAATAAGMRVIGFTGGSHSYLGHSNALVEAGAETAIAKHAHLLEVLEAMAVWQDLP
>Mature_232_residues
PKIDLIIFDCDGVLVDSEYLAAQIGSQLLKKIGYEISPEELSARYAGLIFPDILKKVEQETGKPTSAHLIDQMSDLFRAQ
IKTELRAIDGIKEAIEAIQSHYPYCICSNARSTEIKEMLTIVDLYDFFDNKDKIFSAPEVGTKKTKPAPDVFLFAAQQLR
VKPKNTIVIEDSIHGVHAATAAGMRVIGFTGGSHSYLGHSNALVEAGAETAIAKHAHLLEVLEAMAVWQDLP

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=200, Percent_Identity=28.5, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787576, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788021, Length=186, Percent_Identity=25.2688172043011, Blast_Score=61, Evalue=6e-11,
Organism=Drosophila melanogaster, GI116008157, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 25598; Mature: 25467

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIDLIIFDCDGVLVDSEYLAAQIGSQLLKKIGYEISPEELSARYAGLIFPDILKKVEQ
CCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ETGKPTSAHLIDQMSDLFRAQIKTELRAIDGIKEAIEAIQSHYPYCICSNARSTEIKEML
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
TIVDLYDFFDNKDKIFSAPEVGTKKTKPAPDVFLFAAQQLRVKPKNTIVIEDSIHGVHAA
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCCCHHHH
TAAGMRVIGFTGGSHSYLGHSNALVEAGAETAIAKHAHLLEVLEAMAVWQDLP
HHCCEEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKIDLIIFDCDGVLVDSEYLAAQIGSQLLKKIGYEISPEELSARYAGLIFPDILKKVEQ
CCEEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ETGKPTSAHLIDQMSDLFRAQIKTELRAIDGIKEAIEAIQSHYPYCICSNARSTEIKEML
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
TIVDLYDFFDNKDKIFSAPEVGTKKTKPAPDVFLFAAQQLRVKPKNTIVIEDSIHGVHAA
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCCCHHHH
TAAGMRVIGFTGGSHSYLGHSNALVEAGAETAIAKHAHLLEVLEAMAVWQDLP
HHCCEEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]