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The map label for this gene is moeB [H]

Identifier: 49475053

GI number: 49475053

Start: 318247

End: 319011

Strand: Reverse

Name: moeB [H]

Synonym: BH02410

Alternate gene names: 49475053

Gene position: 319011-318247 (Counterclockwise)

Preceding gene: 49475071

Following gene: 49475048

Centisome position: 16.52

GC content: 41.83

Gene sequence:

>765_bases
ATGATACAAGAAGCTAATACAAAATTAAGCAATGAAGAAATCGAACGCTACGCACGCCACATCATTTTGCCTGAAATTGG
TGGTGTGGGACAACAAAAGCTCAAAGCTGCACGTGTTCTGGTTATTGGCGCAGGCGGTCTTGGTGCTCCTGTTCTCACAT
ATTTGGCGGCTGCTGGCGTTGGCACCCTTGGAATTGTTGATAACGACATCGTTTCACTCTCCAATTTACAACGCCAAGTG
ATTCATAAAACAAACACAATTAACCAATGTAAAACTGACAGTGCTAAAGCCACTATAAACGCAATAAATCCTCATGTTAC
GGTTGAAAAACACACCCTACGCTTAGATAAAAGTAATGTGGGTAAATTGCTCAACGCCTATCACATCATTGTTGATGGGA
GTGATAATTTTGCCACACGCTATCTGCTTGCTGATCATACAGCCCAATGTGCCAAACCTTTAATAAGCGGTGCTGTAGGA
CGTTTTGACGGCTCATTAACAGTGCTCATGCCTTATAAAGACAATAACCCTCACTATCGTGATTTATTCCCAAATCCACC
AGCTCCCGGCACTCTCTCAACATGTGCTGAAACTGGAATTATCGGCGCTTTACCCGGAGTCATTGGAACACTCCAAGCAA
TGGAAGCCATTAAACTTATCACAAACATCGGTGAACCTTTAGTAGGAAAAATCCTCCTTTATAATGGATTATCAGCGCAA
TTTAAAGTTATTACTTATAAGCGATCAGATTCCAATGCCAATTAG

Upstream 100 bases:

>100_bases
GGCAATCTTCATAAATAAAACCTCCCTATAACCGAAAGCGGACTGGTCTATGCGCCATAAACTTAGTAAACTATAAAAGA
ACTATGACAAGAGAAAGCCT

Downstream 100 bases:

>100_bases
CAGCTATCAAGCTTTGCGTGTAAAAATTTGAGAGATTATGATTTACTCTCCAATTTACCTGACTCGAATATATACTGAAA
CCAGAATCATCAGTATTTTA

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIQEANTKLSNEEIERYARHIILPEIGGVGQQKLKAARVLVIGAGGLGAPVLTYLAAAGVGTLGIVDNDIVSLSNLQRQV
IHKTNTINQCKTDSAKATINAINPHVTVEKHTLRLDKSNVGKLLNAYHIIVDGSDNFATRYLLADHTAQCAKPLISGAVG
RFDGSLTVLMPYKDNNPHYRDLFPNPPAPGTLSTCAETGIIGALPGVIGTLQAMEAIKLITNIGEPLVGKILLYNGLSAQ
FKVITYKRSDSNAN

Sequences:

>Translated_254_residues
MIQEANTKLSNEEIERYARHIILPEIGGVGQQKLKAARVLVIGAGGLGAPVLTYLAAAGVGTLGIVDNDIVSLSNLQRQV
IHKTNTINQCKTDSAKATINAINPHVTVEKHTLRLDKSNVGKLLNAYHIIVDGSDNFATRYLLADHTAQCAKPLISGAVG
RFDGSLTVLMPYKDNNPHYRDLFPNPPAPGTLSTCAETGIIGALPGVIGTLQAMEAIKLITNIGEPLVGKILLYNGLSAQ
FKVITYKRSDSNAN
>Mature_254_residues
MIQEANTKLSNEEIERYARHIILPEIGGVGQQKLKAARVLVIGAGGLGAPVLTYLAAAGVGTLGIVDNDIVSLSNLQRQV
IHKTNTINQCKTDSAKATINAINPHVTVEKHTLRLDKSNVGKLLNAYHIIVDGSDNFATRYLLADHTAQCAKPLISGAVG
RFDGSLTVLMPYKDNNPHYRDLFPNPPAPGTLSTCAETGIIGALPGVIGTLQAMEAIKLITNIGEPLVGKILLYNGLSAQ
FKVITYKRSDSNAN

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=233, Percent_Identity=42.4892703862661, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI38045944, Length=153, Percent_Identity=30.718954248366, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI38045942, Length=153, Percent_Identity=30.718954248366, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI38045948, Length=151, Percent_Identity=30.4635761589404, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=41.2244897959184, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082356, Length=241, Percent_Identity=36.9294605809129, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17540406, Length=242, Percent_Identity=40.9090909090909, Blast_Score=201, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI193203301, Length=141, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71984476, Length=153, Percent_Identity=28.1045751633987, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=235, Percent_Identity=42.9787234042553, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6320598, Length=149, Percent_Identity=30.2013422818792, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582879, Length=249, Percent_Identity=41.7670682730924, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI20129973, Length=176, Percent_Identity=31.25, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 27115; Mature: 27115

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQEANTKLSNEEIERYARHIILPEIGGVGQQKLKAARVLVIGAGGLGAPVLTYLAAAGV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHCC
GTLGIVDNDIVSLSNLQRQVIHKTNTINQCKTDSAKATINAINPHVTVEKHTLRLDKSNV
CEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEEEHHEEEECHHHH
GKLLNAYHIIVDGSDNFATRYLLADHTAQCAKPLISGAVGRFDGSLTVLMPYKDNNPHYR
HHHHEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
DLFPNPPAPGTLSTCAETGIIGALPGVIGTLQAMEAIKLITNIGEPLVGKILLYNGLSAQ
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCE
FKVITYKRSDSNAN
EEEEEEECCCCCCC
>Mature Secondary Structure
MIQEANTKLSNEEIERYARHIILPEIGGVGQQKLKAARVLVIGAGGLGAPVLTYLAAAGV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHCC
GTLGIVDNDIVSLSNLQRQVIHKTNTINQCKTDSAKATINAINPHVTVEKHTLRLDKSNV
CEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEEEHHEEEECHHHH
GKLLNAYHIIVDGSDNFATRYLLADHTAQCAKPLISGAVGRFDGSLTVLMPYKDNNPHYR
HHHHEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
DLFPNPPAPGTLSTCAETGIIGALPGVIGTLQAMEAIKLITNIGEPLVGKILLYNGLSAQ
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCE
FKVITYKRSDSNAN
EEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]