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The map label for this gene is rhtB [H]

Identifier: 49474852

GI number: 49474852

Start: 21443

End: 22081

Strand: Reverse

Name: rhtB [H]

Synonym: BH00210

Alternate gene names: 49474852

Gene position: 22081-21443 (Counterclockwise)

Preceding gene: 49474855

Following gene: 49474845

Centisome position: 1.14

GC content: 34.12

Gene sequence:

>639_bases
ATGTCATTTCTTCCTGAATGGCACATTATTATACAATTTTCCTTAGCATCATTGGTTCTGGCCCTCATTCCAGGACCTGA
TATTATGCTATCAGTAGGACGTACTATTGTACAAAATAAAAAAGCTGGAATTATGTGTGCTTGTGGAAGTGCAACGGGTT
TTGCTATTCAGGTCGTTGCTGTAGCTCTTGGCCTATCAGTACTTATTTTTACCTCGCCACGCATTTTTTTTGTTCTCAAA
ATTGTGGGAGCCTTTTACCTTTTGTGGCTTGCTATTCAAACGTTGTATAAACCTTCAACATTATCTTTCAAAAAAACATC
CCCAAAAAACCAAAGCCTCAAACGTAATTATTTTACTGCTATTGTAATTAATCTCTTAAACCCTAAAGTCCTTCTTTTTA
ACATGACTTTCTTACCACAATTTATTAATGCAAATGACCCTATGGTCACACAAAAACTCCTTATTTTAGGTCTTTCTTAT
ATTCCTATTTCTTTTCCTATTAACATTTCTATAGTTTTTATGGCTCATAAATTCTCTAAAGGAATAAAACAAAACCCTTC
TTATCTTCGTTTTCTTGACTGGCTTATCTCAGGCATTTTCACCAGTTTTGCTCTCAGTATCCTTATTGATGAATATTGA

Upstream 100 bases:

>100_bases
AGTATTTAAAAGATGCAAAAAAAAATGAAAACGAGTAAAAAAACATTTATATAAAAGATGCAAATTATGAACATCTTTTT
TAAGAATTAAAGAGGTTTAC

Downstream 100 bases:

>100_bases
ATTATTAACTATTAACTATATGTTCCAAATGCAAACGTTCAGACTTTCGTAACCGTTCTGAAGCAGATTTAAGTTGACCA
CATGCCGCTAAAATATCCCG

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSFLPEWHIIIQFSLASLVLALIPGPDIMLSVGRTIVQNKKAGIMCACGSATGFAIQVVAVALGLSVLIFTSPRIFFVLK
IVGAFYLLWLAIQTLYKPSTLSFKKTSPKNQSLKRNYFTAIVINLLNPKVLLFNMTFLPQFINANDPMVTQKLLILGLSY
IPISFPINISIVFMAHKFSKGIKQNPSYLRFLDWLISGIFTSFALSILIDEY

Sequences:

>Translated_212_residues
MSFLPEWHIIIQFSLASLVLALIPGPDIMLSVGRTIVQNKKAGIMCACGSATGFAIQVVAVALGLSVLIFTSPRIFFVLK
IVGAFYLLWLAIQTLYKPSTLSFKKTSPKNQSLKRNYFTAIVINLLNPKVLLFNMTFLPQFINANDPMVTQKLLILGLSY
IPISFPINISIVFMAHKFSKGIKQNPSYLRFLDWLISGIFTSFALSILIDEY
>Mature_211_residues
SFLPEWHIIIQFSLASLVLALIPGPDIMLSVGRTIVQNKKAGIMCACGSATGFAIQVVAVALGLSVLIFTSPRIFFVLKI
VGAFYLLWLAIQTLYKPSTLSFKKTSPKNQSLKRNYFTAIVINLLNPKVLLFNMTFLPQFINANDPMVTQKLLILGLSYI
PISFPINISIVFMAHKFSKGIKQNPSYLRFLDWLISGIFTSFALSILIDEY

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=186, Percent_Identity=29.5698924731183, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788099, Length=153, Percent_Identity=30.718954248366, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23671; Mature: 23540

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLPEWHIIIQFSLASLVLALIPGPDIMLSVGRTIVQNKKAGIMCACGSATGFAIQVVA
CCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
VALGLSVLIFTSPRIFFVLKIVGAFYLLWLAIQTLYKPSTLSFKKTSPKNQSLKRNYFTA
HHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHH
IVINLLNPKVLLFNMTFLPQFINANDPMVTQKLLILGLSYIPISFPINISIVFMAHKFSK
HHHHHCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEECCEEEEEEEEEHHHH
GIKQNPSYLRFLDWLISGIFTSFALSILIDEY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFLPEWHIIIQFSLASLVLALIPGPDIMLSVGRTIVQNKKAGIMCACGSATGFAIQVVA
CCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
VALGLSVLIFTSPRIFFVLKIVGAFYLLWLAIQTLYKPSTLSFKKTSPKNQSLKRNYFTA
HHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHH
IVINLLNPKVLLFNMTFLPQFINANDPMVTQKLLILGLSYIPISFPINISIVFMAHKFSK
HHHHHCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEECCEEEEEEEEEHHHH
GIKQNPSYLRFLDWLISGIFTSFALSILIDEY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]