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The map label for this gene is act [H]

Identifier: 49186401

GI number: 49186401

Start: 3370823

End: 3371122

Strand: Reverse

Name: act [H]

Synonym: BAS3397

Alternate gene names: 49186401

Gene position: 3371122-3370823 (Counterclockwise)

Preceding gene: 49186402

Following gene: 49186400

Centisome position: 64.47

GC content: 36.67

Gene sequence:

>300_bases
ATGAAAGTGATGAACATTATTCATGATAGTGTAGTAGATGGAGAAGGGTTGCGGACAGTCGTGTTTTTTGCGGGCTGTCC
GCATCGTTGTTTCGGTTGCCATAATCCGAAATCGTGGAATATTTGTAATGGAACTGAAATGACAGTAGAAGAAATTGTGA
AAGAGATTGCAAGTAATCCATTAACTGATGTAACATTTTCAGGTGGAGATCCATTTTTCCAAGCTGCTGAAGTGAAAAAA
GTAGCAAAAGCTGTAAAAGATTTGAAAAAAATTTATGGATGTATACAGGTTATACGTTAG

Upstream 100 bases:

>100_bases
ATATAGAAAGAATTCGCCGTATAACAGGTTATCTTGTAGGAGATATGTCGAAGTGGAATAGTGCGAAACGTAGTGAAGAG
ATGGATCGGGTGAAACATAA

Downstream 100 bases:

>100_bases
AAGAGATACAGAGTTCTCAAAATAATGATATGATAGAGTTGTTACATTATGGGGATGTTTTAGTCGATGGAAGATTTGAA
ATCGGGAAAAAAGATTTAAC

Product: hypothetical protein

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MKVMNIIHDSVVDGEGLRTVVFFAGCPHRCFGCHNPKSWNICNGTEMTVEEIVKEIASNPLTDVTFSGGDPFFQAAEVKK
VAKAVKDLKKIYGCIQVIR

Sequences:

>Translated_99_residues
MKVMNIIHDSVVDGEGLRTVVFFAGCPHRCFGCHNPKSWNICNGTEMTVEEIVKEIASNPLTDVTFSGGDPFFQAAEVKK
VAKAVKDLKKIYGCIQVIR
>Mature_99_residues
MKVMNIIHDSVVDGEGLRTVVFFAGCPHRCFGCHNPKSWNICNGTEMTVEEIVKEIASNPLTDVTFSGGDPFFQAAEVKK
VAKAVKDLKKIYGCIQVIR

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=105, Percent_Identity=36.1904761904762, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 10930; Mature: 10930

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMNIIHDSVVDGEGLRTVVFFAGCPHRCFGCHNPKSWNICNGTEMTVEEIVKEIASNP
CCHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
LTDVTFSGGDPFFQAAEVKKVAKAVKDLKKIYGCIQVIR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVMNIIHDSVVDGEGLRTVVFFAGCPHRCFGCHNPKSWNICNGTEMTVEEIVKEIASNP
CCHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
LTDVTFSGGDPFFQAAEVKKVAKAVKDLKKIYGCIQVIR
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]