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The map label for this gene is yvaE [H]

Identifier: 49186112

GI number: 49186112

Start: 3085726

End: 3086088

Strand: Reverse

Name: yvaE [H]

Synonym: BAS3107

Alternate gene names: 49186112

Gene position: 3086088-3085726 (Counterclockwise)

Preceding gene: 49186113

Following gene: 49186111

Centisome position: 59.02

GC content: 31.68

Gene sequence:

>363_bases
GTGCAGTGGATTTATTTATGTTTAGCGATTTTATTTGAGGTAGCAGGTACAACTGCGATAAAACTATCTGATGGATTAAC
GAAACTTGTACCAAGCATTCTTATTTTTGTATTTTATGTAGTAAGTTTTGCATTTCTGTCATTTGCATTAAAAGGGATGG
AAATGTCCGTTGCATATGCGGTTTGGTCAGGTATGGGAATTGTAACGATTACAACAATTGGTTTTATCTATTTTGGAGAA
AGTATCAATATAATTAAGATAGTAGCAATTCTTTTTATTTTAATTGGAGTCGTAATTTTAAATTTTAATAGTACGGCACA
TGAAGCGAAGAAAAAAGAAATGGTGGAGGAACGTATACATTGA

Upstream 100 bases:

>100_bases
TTATTGAAAAAAGTGATCATACATTAGGAAGTATGCTGGAATCTATTATGGATATGTTACTGTATGGCATTTCTAATTCG
AAATAGAGGGTGGGAGAGTA

Downstream 100 bases:

>100_bases
ATCGATTAAAGTATTTTTTCCTTATTACTGATGTTGGGTTTGTTATATATTGGCTCATAACAATTTTTCATATAATTCCG
CAAGAATATTTGTTTAAAGA

Product: SMR family multidrug efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MQWIYLCLAILFEVAGTTAIKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINIIKIVAILFILIGVVILNFNSTAHEAKKKEMVEERIH

Sequences:

>Translated_120_residues
MQWIYLCLAILFEVAGTTAIKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINIIKIVAILFILIGVVILNFNSTAHEAKKKEMVEERIH
>Mature_120_residues
MQWIYLCLAILFEVAGTTAIKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYAVWSGMGIVTITTIGFIYFGE
SINIIKIVAILFILIGVVILNFNSTAHEAKKKEMVEERIH

Specific function: Multidrug Transporter. Confers Resistance To A Wide Range Of Toxic Compounds By Removing Them For The Cells. The Efflux Is Coupled To An Influx Of Protons. Probably Prevents The Incorporation Of Methyl VIologen Into Cells. Involved In Ethidium Bromide Ef

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=108, Percent_Identity=40.7407407407407, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI87082378, Length=104, Percent_Identity=33.6538461538462, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787884, Length=112, Percent_Identity=36.6071428571429, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1787883, Length=97, Percent_Identity=32.9896907216495, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 13344; Mature: 13344

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWIYLCLAILFEVAGTTAIKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYA
CHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWSGMGIVTITTIGFIYFGESINIIKIVAILFILIGVVILNFNSTAHEAKKKEMVEERIH
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQWIYLCLAILFEVAGTTAIKLSDGLTKLVPSILIFVFYVVSFAFLSFALKGMEMSVAYA
CHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWSGMGIVTITTIGFIYFGESINIIKIVAILFILIGVVILNFNSTAHEAKKKEMVEERIH
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]