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The map label for this gene is 49186069

Identifier: 49186069

GI number: 49186069

Start: 3042691

End: 3042981

Strand: Reverse

Name: 49186069

Synonym: BAS3064

Alternate gene names: NA

Gene position: 3042981-3042691 (Counterclockwise)

Preceding gene: 49186070

Following gene: 49186067

Centisome position: 58.2

GC content: 32.3

Gene sequence:

>291_bases
ATGACATCAACATCTAAAATATGTCACGATTTAAAAGAAGAGGGAATATCAGCCGTTGTTCATATCTTATCAAATTGCGA
AGTTCCTAAGGGTGGAGTAATCACGGAAGAAGGTATATTAGACAATACTATCTATGCAAGCACAATGTGCATGGTGTCTG
GTACATATTATTATCATATCTACGATTGTAGACAAATTACAGCCATTCATTTATTTGATGAAAACTTAGATTCAGAAGAG
ATTAAAACCTATCCGTTCTTGTGTAAACAGAAGATATTTTATGAAAACTAA

Upstream 100 bases:

>100_bases
AGTCTACCATTTAAAGATTTACTTATATAGAATACTAAGCCCTCCTTGTTGTGGAGGGTTTTTATTTATTAATTTTTTAA
GTAATTATTAATTTAATCCA

Downstream 100 bases:

>100_bases
TTAGAATAGGTAAACTGTATACAATACAAATTCAGGCTTCTTATGTGGTTTTAATCTATTTTCAAATGAAAAATCAATAA
CTAATAAACATTACAACCTG

Product: choloylglycine hydrolase

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 96; Mature: 95

Protein sequence:

>96_residues
MTSTSKICHDLKEEGISAVVHILSNCEVPKGGVITEEGILDNTIYASTMCMVSGTYYYHIYDCRQITAIHLFDENLDSEE
IKTYPFLCKQKIFYEN

Sequences:

>Translated_96_residues
MTSTSKICHDLKEEGISAVVHILSNCEVPKGGVITEEGILDNTIYASTMCMVSGTYYYHIYDCRQITAIHLFDENLDSEE
IKTYPFLCKQKIFYEN
>Mature_95_residues
TSTSKICHDLKEEGISAVVHILSNCEVPKGGVITEEGILDNTIYASTMCMVSGTYYYHIYDCRQITAIHLFDENLDSEEI
KTYPFLCKQKIFYEN

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 10966; Mature: 10834

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSKICHDLKEEGISAVVHILSNCEVPKGGVITEEGILDNTIYASTMCMVSGTYYYHI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHEECCEEEEEE
YDCRQITAIHLFDENLDSEEIKTYPFLCKQKIFYEN
EECCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSTSKICHDLKEEGISAVVHILSNCEVPKGGVITEEGILDNTIYASTMCMVSGTYYYHI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHEECCEEEEEE
YDCRQITAIHLFDENLDSEEIKTYPFLCKQKIFYEN
EECCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]