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The map label for this gene is ansB [H]

Identifier: 49185921

GI number: 49185921

Start: 2882279

End: 2883709

Strand: Reverse

Name: ansB [H]

Synonym: BAS2915

Alternate gene names: 49185921

Gene position: 2883709-2882279 (Counterclockwise)

Preceding gene: 49185922

Following gene: 49185919

Centisome position: 55.15

GC content: 38.85

Gene sequence:

>1431_bases
ATGGCAATGACAAACAAGAACGTGCGAATGGAGAAGGATTTTTTGGGAGGAAAGGAATTACCTATTGAGGCATATTACGG
AATTCAGACTTTACGTGCTGTTGAAAATTTTCCGATTACAGGGTATAAAATTCATGAAAGTTTAATTAGAGCGTTCGCAG
TTGTAAAAAAAGCTGCAGCGCTTGCGAATACAGATGTAGGAAGATTGGAATTGAACAAGGGCGGCGCGATCGCAGAAGCT
GCTCAAGAAATTCTTGATGGAAAATGGCATGATCATTTCATCGTAGATCCAATCCAAGGCGGAGCAGGTACTTCAATGAA
CATGAATGCAAATGAAGTCATTGCCAATCGTGCGCTTGAATTATTAGGGATGGAAAAGGGAGACTATCATTATATTAGTC
CAAATAGTCATGTAAACATGGCACAATCAACAAACGATGCATTCCCAACGGCGATTCATATCGCAACATTAAACGCATTA
GAAGGTTTATTACAAACGATGGGTTATATGCACGATGTATTTGAATTAAAAGCAGAACAGTTCGATCATGTGATTAAAAT
GGGGCGTACACATTTACAAGACGCTGTGCCAATTCGTCTTGGACAAGAATTTAAAGCATACTCTCGCGTACTTGAACGTG
ATATGAAACGAATTCAGCAATCGCGTCAACATTTATATGAAGTAAATATGGGAGCAACTGCAGTTGGTACAGGCTTAAAT
GCAGATCCAGAATATATTGAAGCAGTTGTAAAACATTTAGCTGCAATTAGTGAACTGCCACTTGTTGGTGCAGAAGATTT
AGTAGATGCAACGCAAAATACGGATGCATACACAGAAGTATCAGCAGCACTTAAAGTATGTATGATGAATATGTCTAAAA
TTGCGAATGACCTTCGCTTAATGGCATCAGGTCCACGTGTTGGCTTAGCGGAAATTATGCTACCTGCTCGTCAACCAGGT
TCTTCTATTATGCCAGGGAAAGTAAACCCTGTTATGCCAGAAGTAATTAATCAAATTGCATTCCAAGTAATTGGTAACAA
CCATACAATTTGTCTTGCTTCAGAAGCAGGTCAATTAGAATTAAACGTGATGGAACCAGTACTTGTTTTCAACTTACTAC
AATCAATTAGCATTATGAATAACGGTTTCCGTGCCTTTACAGATAATTGCTTAAAAGGAATTGAAGCGAATGAAGATCGC
TTAAAAGAGTATGTTGAGAAGAGTGTAGGAATTATTACAGCCGTGAACCCTCATATCGGTTATGAAGCAGCAGCTCGCGT
TGCGAAAGAAGCAATCGCGACAGGGCAATCCGTTCGAGAACTTTGTGTGAAAAATGGTGTATTGTCACAAGAAGATTTAG
AGTTAATTTTAGATCCATTTGAAATGACACACCCAGGGATTGCAGGAGCAACTCTTTTAAAGAAAAATTAA

Upstream 100 bases:

>100_bases
AGCCTTTATCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAATGACTTCATATGATTTATTTAGGTTTTACATTT
AATATGGGGGAGAAATTAAA

Downstream 100 bases:

>100_bases
AAAAAGAGGGGGGACAAGCCCCTCTTTTTTGTTTACAATATAGAAATGCTATATATGAATAGAGGTAGGGATGTTATGAG
CAAGTTTACAGTAGCTTCTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAMTNKNVRMEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNNHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Sequences:

>Translated_476_residues
MAMTNKNVRMEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNNHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
>Mature_475_residues
AMTNKNVRMEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEAA
QEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALE
GLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLNA
DPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPGS
SIMPGKVNPVMPEVINQIAFQVIGNNHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRL
KEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=42.1505376344086, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI31541964, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303542, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303547, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=49.5708154506438, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.1166306695464, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=43.6008676789588, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.2018348623853, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=42.0258620689655, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640179, Length=469, Percent_Identity=41.5778251599147, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640177, Length=471, Percent_Identity=41.1889596602972, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=41.8259023354565, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=40.9090909090909, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=338, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52004; Mature: 51872

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTNKNVRMEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAA
CCCCCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNNHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
AMTNKNVRMEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAA
CCCCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNNHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]