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The map label for this gene is cobB [H]

Identifier: 49185880

GI number: 49185880

Start: 2844150

End: 2844887

Strand: Reverse

Name: cobB [H]

Synonym: BAS2874

Alternate gene names: 49185880

Gene position: 2844887-2844150 (Counterclockwise)

Preceding gene: 49185881

Following gene: 49185878

Centisome position: 54.41

GC content: 35.37

Gene sequence:

>738_bases
GTGATTTTTGTGCAACAATTTGAAGAAGTACGTACAATTTTAGAAAAAGCGAAGAAAATTACAGTATTAACAGGAGCTGG
TGCAAGTACGGAAAGTGGTATACCAGATTTTCGTTCAGCAAATGGATTGTATGCTGATGCGAATGTGGAAATGTACTTAT
CAAGAGGGTACTATAACCGAAGTCCGAAAGAATTTTGGAAGCATTATAAAGAAATATTTCAAATTAATACGTTTCATCAA
TATAAGCCAAATCGTGGCCATCGCTTTTTAGCTGAATTAGAAGAACAAGGGAAAGATATTACGATTTTAACGCAAAATAT
TGACGGTTTACATCAAGTAGGTGGTAGTAAACATGTCATTGATTTACATGGAACACTGCAAACAGCACATTGTCCGAAGT
GTAAAATGGGATATGATTTGCAGTATATGATAGATCATGAAGTGCCGCGCTGCGAGAAGTGTAATTTCATTTTAAATCCA
GACGTTGTTTTATACGGAGATACATTGCCGCAATATCAAAATGCGATAAAACGTTTATATGAAACAGATGTGCTTATCGT
TATGGGAACGTCACTAAAAGTACAGCCCGTCGCTTCATTCCCGCAAATTGCAAAGAGGGAAGTAGGAGCAACAACCATCT
TAGTAAATGAAGAGTTAACAGGACAGGAATATAACTTTGATTATGTTTTTCAAAATAAGATTGGTGAGTTTGTTGAAGGG
TTATCTTCAATAAAATAA

Upstream 100 bases:

>100_bases
GGATATTGTAGAGGTTGGTGGCACGACGCATTAAGTAGAATGAATTGTACAACACCTCCTGTAAACAACTGGTATAATAA
GTATAGCAATATACATAGAG

Downstream 100 bases:

>100_bases
ATTATCGAAAAAACTGAGGTATCCACCTCAGTTTTTTATTTGGGTAATCGCTGTATTAAGTAACATGAAAACAGGTCGAT
AATGTAGTAACCACAGCTCT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIFVQQFEEVRTILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQ
YKPNRGHRFLAELEEQGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKMGYDLQYMIDHEVPRCEKCNFILNP
DVVLYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEG
LSSIK

Sequences:

>Translated_245_residues
MIFVQQFEEVRTILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQ
YKPNRGHRFLAELEEQGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKMGYDLQYMIDHEVPRCEKCNFILNP
DVVLYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEG
LSSIK
>Mature_245_residues
MIFVQQFEEVRTILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQ
YKPNRGHRFLAELEEQGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKMGYDLQYMIDHEVPRCEKCNFILNP
DVVLYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEG
LSSIK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=38.034188034188, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI13775602, Length=231, Percent_Identity=35.9307359307359, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI13775600, Length=231, Percent_Identity=35.9307359307359, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI6912660, Length=242, Percent_Identity=33.4710743801653, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI63054862, Length=237, Percent_Identity=33.7552742616034, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI6912664, Length=261, Percent_Identity=30.6513409961686, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI300795542, Length=243, Percent_Identity=32.0987654320988, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI13787215, Length=254, Percent_Identity=30.3149606299213, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI300797705, Length=203, Percent_Identity=34.9753694581281, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI215982798, Length=173, Percent_Identity=38.150289017341, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI6912662, Length=226, Percent_Identity=30.5309734513274, Blast_Score=103, Evalue=9e-23,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=25.9842519685039, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI308199517, Length=230, Percent_Identity=33.4782608695652, Blast_Score=100, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17541892, Length=230, Percent_Identity=34.3478260869565, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17567771, Length=227, Percent_Identity=26.8722466960352, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71990482, Length=222, Percent_Identity=27.4774774774775, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=224, Percent_Identity=27.2321428571429, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324599, Length=303, Percent_Identity=28.7128712871287, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324504, Length=282, Percent_Identity=28.3687943262411, Blast_Score=95, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325242, Length=234, Percent_Identity=29.4871794871795, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320163, Length=254, Percent_Identity=27.9527559055118, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320397, Length=231, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137536, Length=256, Percent_Identity=33.59375, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648389, Length=222, Percent_Identity=33.7837837837838, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28571445, Length=186, Percent_Identity=30.1075268817204, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645650, Length=253, Percent_Identity=22.5296442687747, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27979; Mature: 27979

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFVQQFEEVRTILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNR
CCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
SPKEFWKHYKEIFQINTFHQYKPNRGHRFLAELEEQGKDITILTQNIDGLHQVGGSKHVI
CHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCEEE
DLHGTLQTAHCPKCKMGYDLQYMIDHEVPRCEKCNFILNPDVVLYGDTLPQYQNAIKRLY
EECCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHH
ETDVLIVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEG
HCCEEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHH
LSSIK
HHHCC
>Mature Secondary Structure
MIFVQQFEEVRTILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNR
CCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
SPKEFWKHYKEIFQINTFHQYKPNRGHRFLAELEEQGKDITILTQNIDGLHQVGGSKHVI
CHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCEEE
DLHGTLQTAHCPKCKMGYDLQYMIDHEVPRCEKCNFILNPDVVLYGDTLPQYQNAIKRLY
EECCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHH
ETDVLIVMGTSLKVQPVASFPQIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEG
HCCEEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHH
LSSIK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]