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The map label for this gene is nupG [H]

Identifier: 49185786

GI number: 49185786

Start: 2757848

End: 2759041

Strand: Direct

Name: nupG [H]

Synonym: BAS2780

Alternate gene names: 49185786

Gene position: 2757848-2759041 (Clockwise)

Preceding gene: 49185785

Following gene: 49185789

Centisome position: 52.74

GC content: 33.33

Gene sequence:

>1194_bases
ATGTATTTCATATTGAATATGTTAGGGATTTTCGTTGTCATATTAATTGTTTACTTATGTTCGCCTAATAAAAAACATAT
AAAATGGAGACCAATTGTAATTCTCATCATATTAGAGCTTTTTATTACGTGGTTTATGTTAGGCACAAAGCTAGGCAGTA
TTATCATTAATAAAATTGCTTCATTTTTCAGTTGGCTACTGGCATGTGCGAATGAAGGAATTCGATTTGCATTTCCTTCT
GCTATGGAAAATCAGACAATTGATTTCTTCTTTAGCGCATTACTACCTATCATTTTTGTTATCACGTTCTTTGATATTCT
TTCTTACTTTGGAATCTTAACTTGGATTATTGATAAAGTAGGTGCAGTTATTTCAAAGATTTCTCGTTTACCAAAGTTAG
AAAGTTTCTTTTCGATTCAAATGATGTTTTTAGGAAACACTGAAGCACTTGCGGTTGTTCGTGATCAATTATCTGTTTTA
AAAGAAAACCGTTTGCTGACTTTTGGAATTATGAGTATGAGTAGCGTCAGCGGTTCCATTCTTGGTGCTTATTTATCAAT
GGTTCCAGCAACATATATTTTCAGCGCAATCCCATTAAATTGTATTAACGCATTAATTTTAGCCAATGTATTAAATCCTG
TGGAAGTTTCGAAAGAAGAAGATGTTGTTTACACACCTTCCAAACATGAAAAAAAGGATTTCTTTTCTACTATTTCAAAC
AGCATGTTAGTCGGGATGAATATGGTTATCGTTATTTTAGCTATGGTAATTGGTTATGTAGCTTTAACTGCATGTTTAAA
TGGGATTTTAGGATTTTTTGTAACGGGGTTAACAATTCAAAAAATCTTCTCCATTATCTTTAGTCCTTTCGCTTTTTTAC
TCGGTTTATCGGGCAGTGATGCTATGTATGTAGCTGAATTAATGGGGATCAAAATAACGACGAATGAATTTGTTGCAATG
ATGGATTTAAAATCAAACTTAAAGTCTTTACAACCGCATACGGTTGCGGTTGCCACAACATTTCTAGCTTCTTTTGCTAA
CTTTAGTACAGTAGGTATGATTTATGGAACTTACAATTCATTATTTGGCGGCGAAAAATCATCAGTCATCGGTAAAAATG
TTTGGAAGCTTCTTGTGAGCGGAATGGCTGTTTCCTTATTAAGCGCTATGCTTGTTGGGCTTTTTGTATGGTAA

Upstream 100 bases:

>100_bases
TAACATCATTCACATGCTTCATAACTTCTGATCATAATCCAAATTTGGAAGAATAGAGGCTTTTTTATATACAAATAATA
AGCCTCAAGGAGTTGTTGAC

Downstream 100 bases:

>100_bases
ATAATTTTTAAAATAAAAGACACCCAAACGAATTGAGTGTCTTTTATTTTTATTTTCGAATTTGTCCATTTCCACGAATG
ACGTATTTTGTAGAAGTTAA

Product: NupC family nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYFILNMLGIFVVILIVYLCSPNKKHIKWRPIVILIILELFITWFMLGTKLGSIIINKIASFFSWLLACANEGIRFAFPS
AMENQTIDFFFSALLPIIFVITFFDILSYFGILTWIIDKVGAVISKISRLPKLESFFSIQMMFLGNTEALAVVRDQLSVL
KENRLLTFGIMSMSSVSGSILGAYLSMVPATYIFSAIPLNCINALILANVLNPVEVSKEEDVVYTPSKHEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTACLNGILGFFVTGLTIQKIFSIIFSPFAFLLGLSGSDAMYVAELMGIKITTNEFVAM
MDLKSNLKSLQPHTVAVATTFLASFANFSTVGMIYGTYNSLFGGEKSSVIGKNVWKLLVSGMAVSLLSAMLVGLFVW

Sequences:

>Translated_397_residues
MYFILNMLGIFVVILIVYLCSPNKKHIKWRPIVILIILELFITWFMLGTKLGSIIINKIASFFSWLLACANEGIRFAFPS
AMENQTIDFFFSALLPIIFVITFFDILSYFGILTWIIDKVGAVISKISRLPKLESFFSIQMMFLGNTEALAVVRDQLSVL
KENRLLTFGIMSMSSVSGSILGAYLSMVPATYIFSAIPLNCINALILANVLNPVEVSKEEDVVYTPSKHEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTACLNGILGFFVTGLTIQKIFSIIFSPFAFLLGLSGSDAMYVAELMGIKITTNEFVAM
MDLKSNLKSLQPHTVAVATTFLASFANFSTVGMIYGTYNSLFGGEKSSVIGKNVWKLLVSGMAVSLLSAMLVGLFVW
>Mature_397_residues
MYFILNMLGIFVVILIVYLCSPNKKHIKWRPIVILIILELFITWFMLGTKLGSIIINKIASFFSWLLACANEGIRFAFPS
AMENQTIDFFFSALLPIIFVITFFDILSYFGILTWIIDKVGAVISKISRLPKLESFFSIQMMFLGNTEALAVVRDQLSVL
KENRLLTFGIMSMSSVSGSILGAYLSMVPATYIFSAIPLNCINALILANVLNPVEVSKEEDVVYTPSKHEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTACLNGILGFFVTGLTIQKIFSIIFSPFAFLLGLSGSDAMYVAELMGIKITTNEFVAM
MDLKSNLKSLQPHTVAVATTFLASFANFSTVGMIYGTYNSLFGGEKSSVIGKNVWKLLVSGMAVSLLSAMLVGLFVW

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=412, Percent_Identity=27.9126213592233, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI227116277, Length=410, Percent_Identity=27.3170731707317, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI11545853, Length=411, Percent_Identity=26.5206812652068, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1788737, Length=393, Percent_Identity=34.6055979643766, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1788485, Length=416, Percent_Identity=26.4423076923077, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788488, Length=417, Percent_Identity=26.6187050359712, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560276, Length=396, Percent_Identity=26.010101010101, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71991794, Length=397, Percent_Identity=24.9370277078086, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552519, Length=397, Percent_Identity=25.4408060453401, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45552517, Length=397, Percent_Identity=25.4408060453401, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19921868, Length=397, Percent_Identity=25.4408060453401, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281360430, Length=407, Percent_Identity=23.5872235872236, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43837; Mature: 43837

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFILNMLGIFVVILIVYLCSPNKKHIKWRPIVILIILELFITWFMLGTKLGSIIINKIA
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFFSWLLACANEGIRFAFPSAMENQTIDFFFSALLPIIFVITFFDILSYFGILTWIIDKV
HHHHHHHHHHCCCCEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVISKISRLPKLESFFSIQMMFLGNTEALAVVRDQLSVLKENRLLTFGIMSMSSVSGSI
HHHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
LGAYLSMVPATYIFSAIPLNCINALILANVLNPVEVSKEEDVVYTPSKHEKKDFFSTISN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCEEECCCCCHHHHHHHHHHH
SMLVGMNMVIVILAMVIGYVALTACLNGILGFFVTGLTIQKIFSIIFSPFAFLLGLSGSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMYVAELMGIKITTNEFVAMMDLKSNLKSLQPHTVAVATTFLASFANFSTVGMIYGTYNS
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCHHH
LFGGEKSSVIGKNVWKLLVSGMAVSLLSAMLVGLFVW
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYFILNMLGIFVVILIVYLCSPNKKHIKWRPIVILIILELFITWFMLGTKLGSIIINKIA
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFFSWLLACANEGIRFAFPSAMENQTIDFFFSALLPIIFVITFFDILSYFGILTWIIDKV
HHHHHHHHHHCCCCEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVISKISRLPKLESFFSIQMMFLGNTEALAVVRDQLSVLKENRLLTFGIMSMSSVSGSI
HHHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
LGAYLSMVPATYIFSAIPLNCINALILANVLNPVEVSKEEDVVYTPSKHEKKDFFSTISN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCEEECCCCCHHHHHHHHHHH
SMLVGMNMVIVILAMVIGYVALTACLNGILGFFVTGLTIQKIFSIIFSPFAFLLGLSGSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMYVAELMGIKITTNEFVAMMDLKSNLKSLQPHTVAVATTFLASFANFSTVGMIYGTYNS
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCHHH
LFGGEKSSVIGKNVWKLLVSGMAVSLLSAMLVGLFVW
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]