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The map label for this gene is tenA [H]

Identifier: 49185444

GI number: 49185444

Start: 2433872

End: 2434561

Strand: Direct

Name: tenA [H]

Synonym: BAS2436

Alternate gene names: 49185444

Gene position: 2433872-2434561 (Clockwise)

Preceding gene: 49185443

Following gene: 49185445

Centisome position: 46.55

GC content: 35.8

Gene sequence:

>690_bases
ATGACTTTTTCACAATCATTACGTAAAGAAGTAGATTCAATTTGGGAAGCTAGTTTTCATCATCCTTTTGTAAAAAAACT
CGGTGAAGGAACGTTAGATTTAGCTAATTTTCGCTATTACGTGCTTCAAGATTCCTATTATTTGAGTCACTTTGCTAGAG
TACAAACATTAGGAGCTGCAAAAGCGCTTGAATTAGAAACGACTGCTCGCATGGCACATCATGCTCAAAATACATATGAG
GCGGAATTATCTTTACATGAGAACTTTGCGAAGAAACTAGGGATTACACAAGAAGAAAAAGATAATTTTATCCCTGCTCC
AACTGCATATGCATATACTTCACATATGTACCGTGCTGCGTATGAAGGACATTTAGGCGATATTATTGCAGCAATTTTAC
CTTGCTATTGGCTGTATTATGAAATTGGTGAGCGTTTAAAAGAGTGTCAGCCAGAAGAGCCGATTTATAACGAATGGATT
TCTGCTTATGGATCTGATTGGTTCCGAACATTAGTTGAAGAGCAAATTACACGATTAGATGCAATAGCTGAAAAAGTGAC
AGAGTCTGATCGTAAGCGTATGAAACAGCATTTTGTTATAAGTAGTCAATATGAATATTCATTTTGGGAAATGGCTTATA
CTTTAGAAAAGTGGCCGGTAGATACAGAAGTAAAAGATGTAATAAGATAA

Upstream 100 bases:

>100_bases
ATTTCACTTTCTTTACGCCTGTAAAGAAAGTTTTTTTATTATAAAGCGTTAATTGCTGTGGATAAATACTTATATGAAAT
GGATTATTGGAGGGAATCAA

Downstream 100 bases:

>100_bases
AGAAAAGAGGTATTGCTATACGTATGTATGGACAATACCTCTTTTTAGGTTTTTATAAAATCATTGTAAAAATCACAAAT
AAAACACTCCTTTATATTGT

Product: transcriptional activator TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYE
AELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWI
SAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Sequences:

>Translated_229_residues
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYE
AELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWI
SAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
>Mature_228_residues
TFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARMAHHAQNTYEA
ELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWIS
AYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=26.7326732673267, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=201, Percent_Identity=21.8905472636816, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26912; Mature: 26780

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAA
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEECHHHHHHHHHHHHHHHH
KALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHH
YEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
AIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAA
CHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEECHHHHHHHHHHHHHHHH
KALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHH
YEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
AIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]