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The map label for this gene is rob [H]

Identifier: 49185414

GI number: 49185414

Start: 2405265

End: 2406368

Strand: Direct

Name: rob [H]

Synonym: BAS2406

Alternate gene names: 49185414

Gene position: 2405265-2406368 (Clockwise)

Preceding gene: 49185412

Following gene: 49185416

Centisome position: 46.0

GC content: 29.98

Gene sequence:

>1104_bases
GTGGAGGAAATGAATGAACATATACAGCAGATGATTGACTGGATTGAGGTCAATTTAAAAAAGGAATTTTCATTAGATGA
ATTGTCCCGTTATATGGGGTATTCTCCTTATTATTGTTCTTTTAAATTTCACCAAGTAACGGGTTTCAGTATTAGACGCT
ATATTCTTCTTAGAAGGTTATATTTATCTATAGAAGATTTAAAGAATGGTAGGAAAATAATTGAGATCGCATTGGATTAT
GATTACTCTTCCCAAGAGGCTTATAGTAGAGCTTTCAAAAATGTTTTTGGAATGAATCCAAGAGAATACCAACTTAGCAA
AATGCCTATTCAATCATTTGTTAAACTCAATTTAAATAATGAAGGGGCGTTTAATGTGAATATTTCTAGAAAAATAGAGG
TTGAACAGTTGCGAAATAGGAAGAGTGAGCTATTTGATAAAGAGGTACTTAACATATTAAATGGTCAACTTATGTATGAA
GAATTTAAAAACGAAAAGCTAATGGGTGATTCTGATTATGCACCATTTAATGAAGCGATGTGTGTAAACCGAGTTACTAC
ACTAGTTTTTGATGAAGAATTTATTAAGACAAGGGCAGCAGGACATAACAGTTCAGTAGAAAGTTACACAAATAAGGTTA
TAGATCCATTAAAGAAACTTTTTACGAAAGAGTATAAATGTATTGTTTTATGGTTTGGTGAAGATATGTTCTGCCAAATG
AACTTACTTACCATACTTTCATACCTTGAACATTCTCGTTATGAGGGGAAGTTATACTTAAATAGCTTCAGAGAAGATGA
ATTTAAAGTAAATCAAATTGAACTTGAATTAGGAAAATATTCTTCGGTATACAATGAAGTATTAGTAAATCATAAAAAGA
CTTTCTATAAGGTACCACCTGTAATGTATCAGGCTATAGATTTATATTTAGAAATGCTAAAAGAAGATAATGCAGTAATA
AAATTCATCTCTAGAAATAAAGATTTATCAACTCGTGAATTATTAATAAAGTTGTTTCATCTTTTTCCAACAATTGGATA
TGGAGATACTCAGTACATAGAGCTGATTAATAAAATAAAGAAGAAAGCTACACCTAAAATATAG

Upstream 100 bases:

>100_bases
TATATATAAAATTTAAAGATATTACAGAAAGTCTCATGTTATAAAACACAAGAAATATCAAAAGAAAGAAATGCACTTCT
CTTATACTTTTAAATAAGGA

Downstream 100 bases:

>100_bases
GTATAGTTTTCTTCTTATAATTGCTCAAGGAATTCTAATTCTTTTTCTACAAATAGCATTAATTCTTTAATTGTTTCGCC
AGAAAAATCAAAACGAATAC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEEMNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDY
DYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYE
EFKNEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQM
NLLTILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVI
KFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI

Sequences:

>Translated_367_residues
MEEMNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDY
DYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYE
EFKNEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQM
NLLTILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVI
KFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI
>Mature_367_residues
MEEMNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRLYLSIEDLKNGRKIIEIALDY
DYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNNEGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYE
EFKNEKLMGDSDYAPFNEAMCVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQM
NLLTILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPPVMYQAIDLYLEMLKEDNAVI
KFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIKKKATPKI

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081928, Length=106, Percent_Identity=33.9622641509434, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 43629; Mature: 43629

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEMNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHH
YLSIEDLKNGRKIIEIALDYDYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNN
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCC
EGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFKNEKLMGDSDYAPFNEAM
CCEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHH
CVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQM
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
NLLTILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPP
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHEEECCH
VMYQAIDLYLEMLKEDNAVIKFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIK
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KKATPKI
HHCCCCC
>Mature Secondary Structure
MEEMNEHIQQMIDWIEVNLKKEFSLDELSRYMGYSPYYCSFKFHQVTGFSIRRYILLRRL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHH
YLSIEDLKNGRKIIEIALDYDYSSQEAYSRAFKNVFGMNPREYQLSKMPIQSFVKLNLNN
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCC
EGAFNVNISRKIEVEQLRNRKSELFDKEVLNILNGQLMYEEFKNEKLMGDSDYAPFNEAM
CCEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHH
CVNRVTTLVFDEEFIKTRAAGHNSSVESYTNKVIDPLKKLFTKEYKCIVLWFGEDMFCQM
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
NLLTILSYLEHSRYEGKLYLNSFREDEFKVNQIELELGKYSSVYNEVLVNHKKTFYKVPP
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHEEECCH
VMYQAIDLYLEMLKEDNAVIKFISRNKDLSTRELLIKLFHLFPTIGYGDTQYIELINKIK
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KKATPKI
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]