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The map label for this gene is yiaE [C]

Identifier: 49184342

GI number: 49184342

Start: 1356420

End: 1357391

Strand: Direct

Name: yiaE [C]

Synonym: BAS1325

Alternate gene names: 49184342

Gene position: 1356420-1357391 (Clockwise)

Preceding gene: 49184341

Following gene: 49184345

Centisome position: 25.94

GC content: 37.45

Gene sequence:

>972_bases
GTGGCAAAAATATTAGTAGCAGGAAAAATTCCCGAAATCGGATTAGAACTATTAAAAGATCATGATGTGGAAATGTATGA
TAAAGAGGAGTTAATTTCTTTAGATGAATTAACAGAGCGTGTAAAAGACAAAGATGCATTGCTAAGCCTACTTTCTACAA
AGGTGACAAAAGAAGTGATTGATGCGGCACCTAGTTTGAAGATTGTTGCAAACTACGGTGCTGGTTACGATAATATTGAT
TACACGTATGCTGGAGAAAAAGGGATTGCGGTAACGAATACACCGAAAGTATCAACGGAAGCGACAGCAGAATTAACATT
TGCACTTCTTTTAGCAGCTGCACGTAGAATTCCTGAAGGTGATACGCTATGTCGTACAACGGGATTTAACGGATGGGCAC
CGTTATTTTTCTTAGGCCGAGAAGTACATGGTAAAACAATTGGAATTATTGGCCTTGGAGAAATCGGAAAGGCAGTTGCA
AAACGTGCGAAAGCATTTGGAATGAATATTTTATATACAGGACCAAATCGAAAACCTGAAGCTGAAAGTGAACTGGAAGC
AACATATGTAACATTGGAAGAGTTATTACAAACAGCAGACTTTATTACAATTAACTGTGCGTATAATCCGAAATTGCATC
ATATGATTGATGAAGAGCAGTTTAAAATGATGAAGAAAACAGCGTATATTGTAAATGCTTCACGTGGACCAATTATGCAC
GAAGCGGCACTTGCTCATGCGTTAAAAACGAATGAAATTGAAGGAGCAGCTCTTGACGTGTTTGAATTTGAGCCGAAAAT
TACTGAAGAGTTAAAAGGATTAAAGAATGTAGTACTTGCTCCTCACGTAGGAAATGCAACATTTGAAACGCGTGATGCGA
TGGCTGAAATGGCAGTAAGAAATATTTTAGCTGTATTAAAGGGTGAAGAACCTGTAACACCTGTAAATCAAAAAGTATTC
GTTACAAAATAA

Upstream 100 bases:

>100_bases
ATTTTTAAAACTATAATCAAATTCCTTGGTTTTTATATGTGGATTTCTTACAATGAAAGATAAGAGATATCCTATTTTGT
ATAGAAGAGGTGTTAGTATA

Downstream 100 bases:

>100_bases
AAAGAAACCTTCCAGTTCCGGAAGGTTTCTTTTTATTTTTCTTTATTTGCTCTTTTTGATGGTCTTTGTCTAAGGAATTG
AGATAGCATGTACAAAATAT

Product: glycerate dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAPSLKIVANYGAGYDNID
YTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVA
KRAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMH
EAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILAVLKGEEPVTPVNQKVF
VTK

Sequences:

>Translated_323_residues
MAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAPSLKIVANYGAGYDNID
YTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVA
KRAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMH
EAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILAVLKGEEPVTPVNQKVF
VTK
>Mature_322_residues
AKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVIDAAPSLKIVANYGAGYDNIDY
TYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAK
RAKAFGMNILYTGPNRKPEAESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMHE
AALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVRNILAVLKGEEPVTPVNQKVFV
TK

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=38.7301587301587, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI23308577, Length=322, Percent_Identity=33.5403726708075, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4557497, Length=320, Percent_Identity=35.3125, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI61743967, Length=320, Percent_Identity=35.3125, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI145580578, Length=334, Percent_Identity=33.2335329341317, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4557499, Length=334, Percent_Identity=33.2335329341317, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI145580575, Length=333, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=38.4328358208955, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789279, Length=265, Percent_Identity=32.0754716981132, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787645, Length=259, Percent_Identity=31.2741312741313, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788660, Length=257, Percent_Identity=29.1828793774319, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=307, Percent_Identity=33.5504885993485, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147481, Length=318, Percent_Identity=30.5031446540881, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=245, Percent_Identity=37.1428571428571, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6322116, Length=313, Percent_Identity=31.3099041533546, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=31.6455696202532, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324964, Length=322, Percent_Identity=27.0186335403727, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=29.6511627906977, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571528, Length=306, Percent_Identity=40.1960784313725, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28574286, Length=318, Percent_Identity=35.5345911949685, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45552429, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24585514, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI28574282, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI28574284, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45551003, Length=317, Percent_Identity=34.384858044164, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI19921140, Length=315, Percent_Identity=34.2857142857143, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24646446, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646448, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646452, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646450, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI62472511, Length=319, Percent_Identity=34.1692789968652, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24585516, Length=317, Percent_Identity=29.3375394321767, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 35389; Mature: 35258

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVI
CCEEEEECCCCHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAPSLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEG
HCCCCEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAKAFGMNILYTGPNRKPE
CCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
AESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMH
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHEEEECCCCCCHH
EAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVR
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
NILAVLKGEEPVTPVNQKVFVTK
HHHHHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AKILVAGKIPEIGLELLKDHDVEMYDKEELISLDELTERVKDKDALLSLLSTKVTKEVI
CEEEEECCCCHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAPSLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEG
HCCCCEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAKAFGMNILYTGPNRKPE
CCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
AESELEATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMH
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHEEEECCCCCCHH
EAALAHALKTNEIEGAALDVFEFEPKITEELKGLKNVVLAPHVGNATFETRDAMAEMAVR
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
NILAVLKGEEPVTPVNQKVFVTK
HHHHHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]