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The map label for this gene is glcF [H]

Identifier: 49184227

GI number: 49184227

Start: 1254843

End: 1256171

Strand: Reverse

Name: glcF [H]

Synonym: BAS1209

Alternate gene names: 49184227

Gene position: 1256171-1254843 (Counterclockwise)

Preceding gene: 49184228

Following gene: 49184226

Centisome position: 24.02

GC content: 37.77

Gene sequence:

>1329_bases
ATGACAACATTAAATAAAGAAAACATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAACTACTAAACTGTATGCG
ATGCGGTTTCTGTTTACCAACTTGCCCTACTTACATTCAATCTGGATATAAAGAATCACATTCCCCGCGCGGACGTATTG
CATTAATGAAAGCGGTCGTTGACGGTTTAATTGAGCCAGATGAAGATGTTGAAAACACATTAAACGTTTGCCTTGGCTGC
CGCGCTTGTGAACCTGTTTGTCCATCTGGTGTGAATTATGGCCATTTATTAGAAGAAGCTCGTGATATTATAAATCAAAA
CAAAAAGTTCTCGATGCCAGTGAGAGCCGTTCGTAAAGTCGTTTTTGAAGAGCTATTCCCGCATCAAAATCGAATGAGAA
CATTAACCGGATTAATTGGGTTTTATCAGCGTTCTGGTTTACAAACGATAACACATAAAACGGGAATTATGAAGTTATTC
CCTGAAACACTTGCAACGATGGATCTCGTTTTACCAAAAGTCCCTAAAATGAAAGCAATGAAAGATCGTCCTACATTTTT
ACCTGCTGAAAGTACAAAGAAGAAACAAGTTGCATTCTTCACAGGTTGTTTAATGGATACGATGTTTTTAGAAACAAATA
ACGCAACGATGAAACTACTTCAGTTAGCTGGTTGTGATATCGTTATTCCGAAAACGCAAAGTTGCTGCGGGGCATTACAT
GGGCATGCTGGTGAGAAAAGTGGAGCAAAAGAATTAGCGAAACGCAATATAAAAGCATTCGAAGATTTAAACATTGACTA
TATTATTACGAATGCTGGTGGTTGCGGAGCTTACCTCGTAGACTACGATTATCTTTTAAAAGATGACCCACAGTGGGCTG
GACGTGCAAAACAGTTTGTTTCCAAAATTAAAGATATTACTGCTATTCTAGTAGAACTAGATTTCCATAAACGTAATGAC
TTACGACTCACGCCGCAAATTATTACGTATCAAGACTCTTGTCATTTACGCAATGTCATGCGAACATCTTCAGAACCTCG
TATGTTACTAGAAGCAATTCAAGGGGCAACATACCGTGAAATGAAAGACGCTGATCGCTGCTGTGGTTCTGCTGGTATTT
ATAACATTGTTCATTCAGAACTATCAATGGAATTTCTAGATTACAAAATGGACCGTGTGCATGAAACAGACGCAACAACC
ATTGTTACTGCGAATCCAGGTTGTTTATTACAAATGAAATTAGGCATTGAACGAGAGGGTCTTTCTCATAAAATGAGAGG
AATTCACATTGTTGATTTATTATTAGAAGCGATTGAAACCAACTCGTAA

Upstream 100 bases:

>100_bases
CCGCTATGCAAGGAATTAAACACGCCTTCGATCCAAATAACATTATGAATCCAGGAAAGATGTTCGCGAAGGACACTCGT
AAAAGAGTGGTGGTTGAGCG

Downstream 100 bases:

>100_bases
TAACTCGTAACATACGTAATAATAGGAAAACGGCTATCTCCTTCGTCTATGTAAAGAGATAGCCGCTTTTTTGTCCGCTA
CAACATTAAAAATATGTTAC

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEELFPHQNRMRTLTGLIGFYQRSGLQTITHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPTFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAGRAKQFVSKIKDITAILVELDFHKRND
LRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Sequences:

>Translated_442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEELFPHQNRMRTLTGLIGFYQRSGLQTITHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPTFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAGRAKQFVSKIKDITAILVELDFHKRND
LRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS
>Mature_441_residues
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGCR
ACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEELFPHQNRMRTLTGLIGFYQRSGLQTITHKTGIMKLFP
ETLATMDLVLPKVPKMKAMKDRPTFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALHG
HAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAGRAKQFVSKIKDITAILVELDFHKRNDL
RLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTI
VTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=451, Percent_Identity=29.0465631929047, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786497, Length=243, Percent_Identity=28.8065843621399, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 49773; Mature: 49642

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEELFPHQNRMRTLTGLIGFYQRSGLQTITHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPTFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAGRAKQFV
CCCCCCCHHHHHHHHCHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCCCHHHHHHHHH
SKIKDITAILVELDFHKRNDLRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEELFPHQNRMRTLTGLIGFYQRSGLQTITHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPTFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAGRAKQFV
CCCCCCCHHHHHHHHCHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCCCHHHHHHHHH
SKIKDITAILVELDFHKRNDLRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]