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The map label for this gene is fabI [H]

Identifier: 49184159

GI number: 49184159

Start: 1190361

End: 1191131

Strand: Direct

Name: fabI [H]

Synonym: BAS1139

Alternate gene names: 49184159

Gene position: 1190361-1191131 (Clockwise)

Preceding gene: 49184158

Following gene: 49184160

Centisome position: 22.77

GC content: 39.82

Gene sequence:

>771_bases
ATGGAACTATTACAAGGGAAAACATTTGTTGTTATGGGCGTTGCGAACCAAAGAAGTATTGCATGGGGAATTGCTCGCTC
TTTGCATAATGCAGGTGCAAAATTAATCTTCACATATGCAGGAGAACGTTTAGAGAGAAATGTTCGTGAATTAGCGGATA
CATTAGAAGGACAAGAATCACTTGTATTACCTTGTGATGTAACAAATGATGAAGAACTTACAGCTTGTTTTGAAACAATT
AAGCAAGAAGTTGGTACAATTCACGGTGTTGCACACTGTATTGCTTTTGCAAATCGTGACGACTTAAAAGGTGAATTTGT
AGATACTTCTCGCGATGGATTTTTACTTGCACAAAATATTAGTGCATTCTCTTTAACAGCTGTAGCAAGAGAAGCGAAGA
AAGTAATGACAGAAGGCGGAAATATTTTAACGTTAACATATCTTGGCGGCGAGCGCGTTGTGAAAAACTATAACGTTATG
GGTGTTGCGAAAGCTTCATTAGAAGCGAGCGTGAAATATTTAGCTAACGATTTAGGCCAACACGGCATTCGCGTTAACGC
TATTTCTGCAGGTCCAATTCGTACGTTATCTGCCAAAGGTGTAGGCGACTTTAACTCAATCTTAAGAGAAATTGAGGAGC
GCGCACCACTTCGTCGTACAACAACGCAAGAAGAAGTTGGGGATACAGCAGTATTCTTATTCAGTGATTTAGCACGCGGC
GTAACAGGAGAAAACATTCACGTTGATTCAGGGTATCATATCTTAGGATAA

Upstream 100 bases:

>100_bases
AGTTAATGACTTTTTTGTTTGCCTTTAAGAGGTTTTATGTTACTATAATTATAGTATCAGGTACTAATAACAAGTATAAG
TATTTCTGGGAGGATATATC

Downstream 100 bases:

>100_bases
ATATAATATTAATTTTAAAGGACAATCTCTACATGTTGAGATTGTCCTTTTTATTTGTTCTTAGAAAGAACGATTTTTAA
CGAAAGTTCTTACCACGTTA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQESLVLPCDVTNDEELTACFETI
KQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNISAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVM
GVAKASLEASVKYLANDLGQHGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARG
VTGENIHVDSGYHILG

Sequences:

>Translated_256_residues
MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQESLVLPCDVTNDEELTACFETI
KQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNISAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVM
GVAKASLEASVKYLANDLGQHGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARG
VTGENIHVDSGYHILG
>Mature_256_residues
MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQESLVLPCDVTNDEELTACFETI
KQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNISAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVM
GVAKASLEASVKYLANDLGQHGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARG
VTGENIHVDSGYHILG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=47.0588235294118, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=29.0076335877863, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=27.5590551181102, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=24.8995983935743, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=28.2352941176471, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI2367365, Length=257, Percent_Identity=25.6809338521401, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=239, Percent_Identity=27.1966527196653, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27741; Mature: 27741

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQES
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCC
LVLPCDVTNDEELTACFETIKQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECCC
SAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVMGVAKASLEASVKYLANDLGQ
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHC
HGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARG
CCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCC
VTGENIHVDSGYHILG
CCCCEEEECCCCEECC
>Mature Secondary Structure
MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQES
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCC
LVLPCDVTNDEELTACFETIKQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEECCC
SAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVMGVAKASLEASVKYLANDLGQ
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCEEEEEHHHHHHHHHHHHHHHHC
HGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARG
CCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCC
VTGENIHVDSGYHILG
CCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]