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The map label for this gene is rfbB [H]

Identifier: 49184157

GI number: 49184157

Start: 1188418

End: 1189386

Strand: Direct

Name: rfbB [H]

Synonym: BAS1137

Alternate gene names: 49184157

Gene position: 1188418-1189386 (Clockwise)

Preceding gene: 49184156

Following gene: 49184158

Centisome position: 22.73

GC content: 35.4

Gene sequence:

>969_bases
ATGAATATATTAGTAACGGGTGGGGCCGGATTTATTGGAAGCAATTTTGTACATTATATGTTACAAAGCTATGAAACATA
TAAAATTATTAATTTTGATGCATTAACATATAGTGGAAACTTAAATAATGTAAAGTCTATTCAAGATCATCCGAATTACT
ATTTTGTAAAAGGGGAAATTCAAAATGGAGAGCTGCTGGAGCATGTTATTAAGGAACGTGATGTGCAAGTAATCGTCAAT
TTCGCAGCTGAATCACATGTGGATCGTAGTATTGAAAATCCAATCCCTTTTTATGATACAAATGTAATTGGGACGGTCAC
CTTACTAGAATTAGTAAAAAAGTATCCGCATATTAAACTTGTGCAAGTATCAACGGATGAGGTGTATGGCTCGTTAGGAA
AAACAGGACGATTTACCGAGGAAACACCGTTAGCTCCAAATAGTCCATATTCTTCAAGCAAAGCGAGCGCCGATATGATA
GCTTTAGCTTATTATAAAACATATCAATTACCAGTTATAGTAACGCGTTGTTCCAATAACTATGGGCCGTATCAATACCC
TGAAAAATTAATCCCGTTAATGGTTACGAATGCGCTCGAAGGAAAAAAATTACCGTTATATGGCGATGGATTGAATGTAA
GAGATTGGTTACATGTAACAGATCATTGTAGTGCGATTGACGTTGTTCTGCATAAGGGACGTGTAGGAGAAGTATATAAT
ATAGGCGGAAATAATGAAAAAACAAATGTAGAAGTTGTGGAACAAATTATTACTCTCTTAGGAAAAACAAAAAAAGATAT
TGAATATGTGACAGACCGTTTAGGACACGACCGTCGTTATGCGATTAACGCAGAGAAAATGAAGAATGAATTTGATTGGG
AACCAAAGTATACGTTTGAGCAAGGATTGCAAGAAACGGTGCAATGGTATGAGAAGAATGAGGAATGGTGGAAACCATTA
AAAAAGTAA

Upstream 100 bases:

>100_bases
ATTCCATGGCCGGTAACAAGTCCTATTTTGTCTGATAAAGATCGAATATTACCATTACTTCAGGAATGTGAAGATAGCTT
TTGAGTAGGAGAGTTGTTAT

Downstream 100 bases:

>100_bases
GGGGCATATAAATGAAAGAGCGGGTTATCATAACAGGAGCAAATGGTCAATTAGGAAAGCAACTACAAGAAGAGTTAAAT
CCTGAAGAATATGACATATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAINAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Sequences:

>Translated_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAINAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK
>Mature_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAINAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=38.2262996941896, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=28.0373831775701, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI56237023, Length=342, Percent_Identity=26.0233918128655, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI56118217, Length=342, Percent_Identity=26.0233918128655, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI189083684, Length=342, Percent_Identity=26.0233918128655, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4504031, Length=335, Percent_Identity=25.0746268656716, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=47.3529411764706, Blast_Score=320, Evalue=7e-89,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=45.7478005865103, Blast_Score=310, Evalue=7e-86,
Organism=Escherichia coli, GI1786974, Length=346, Percent_Identity=26.5895953757225, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788366, Length=231, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788589, Length=356, Percent_Identity=23.0337078651685, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=38.5093167701863, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=33.0188679245283, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=24.9240121580547, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=26.4788732394366, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.7045454545455, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=346, Percent_Identity=27.7456647398844, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356223, Length=326, Percent_Identity=28.2208588957055, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19923002, Length=344, Percent_Identity=28.1976744186047, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36861; Mature: 36861

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHCCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHEEHHHCCHHHHHHHHCCCCCCEEE
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAINAEKMKNEFDWEPKYTFE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHCCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHEEHHHCCHHHHHHHHCCCCCCEEE
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAINAEKMKNEFDWEPKYTFE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]