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The map label for this gene is prpE

Identifier: 49184144

GI number: 49184144

Start: 1177490

End: 1178230

Strand: Reverse

Name: prpE

Synonym: BAS1124

Alternate gene names: 49184144

Gene position: 1178230-1177490 (Counterclockwise)

Preceding gene: 49184145

Following gene: 49184143

Centisome position: 22.53

GC content: 40.89

Gene sequence:

>741_bases
ATGAAATATGACATTATAGGAGATATACATGGATGCTTACAAGAGTTTCAAAATTTAACAGAGAAGCTAGGGTATAACTG
GAGTAGCGGTCTACCGGTTCACCCTGATCAACGCAAACTTGCATTTGTTGGTGATATTACAGATCGCGGCCCTCATTCAT
TACGTATGATTGAAATTGTATGGGAACTTGTCATACATAAAAAAGTAGCTTACTACGCTCCGGGAAATCACTGTAATAAA
CTATACCGATTTTTCCTTGGGCGTAATGTAACAATCGCTCACGGACTTGAAACAACTGTCGCTGAATATGAGGCACTTCC
TTCTCATAAACAAAACATGATAAAAGAAAAATTCATCACACTTTACGAGCAATCCCCTTTATATCACATACTCGATGAGA
AAAGGTTACTCGTGTGTCACGCCGGCATTCGGCAAGATTACATAGGAAGACAAGATAAAAAAGTACAAACCTTTGTATTA
TATGGCGATATTACAGGAGAAAAACATGCTGACGGCTCACCTGTCCGCCGCGACTGGGCGAAGGAGTATAAAGGAACAAC
TTGGATTGTATATGGACATACACCCGTGAAAGAACCTCGCTTTGTAAATCATACAGTCAATATTGATACTGGCGCTGTAT
TTGGTGGCAGACTAACTGCGCTACGTTATCCAGAAATGGAGACCGTTTCCGTTCCTTCCTCCCTGCCTTTTGTCCCTGAG
AAATTTCGCCCAATTTCATAA

Upstream 100 bases:

>100_bases
GAACGAAAATCTTTATGTTTAACTAAGGCACAAAAACCGTATATTTTTATCTCTATCCTTTTTTAGATATACTAATGAAA
AATATGATGGAGGTTTAAAT

Downstream 100 bases:

>100_bases
CCCATATGTAATACTTACGAAGGATTACCGTATCATTTAGGCGCCTCTCATATAAAAACTGGGTAAAGTCATTAAAAAAG
ATGCAATAAAGTGCGTCTTT

Product: bis(5'-nucleosyl)-tetraphosphatase PrpE

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHILDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGRLTALRYPEMETVSVPSSLPFVPE
KFRPIS

Sequences:

>Translated_246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHILDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGRLTALRYPEMETVSVPSSLPFVPE
KFRPIS
>Mature_246_residues
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKVAYYAPGNHCNK
LYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFITLYEQSPLYHILDEKRLLVCHAGIRQDYIGRQDKKVQTFVL
YGDITGEKHADGSPVRRDWAKEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGRLTALRYPEMETVSVPSSLPFVPE
KFRPIS

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPE_BACAA (C3P3Q5)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002865734.1
- ProteinModelPortal:   C3P3Q5
- EnsemblBacteria:   EBBACT00000127959
- GeneID:   7852762
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1292
- GeneTree:   EBGT00050000002430
- ProtClustDB:   PRK13625
- HAMAP:   MF_01443
- InterPro:   IPR004843

Pfam domain/function: PF00149 Metallophos

EC number: =3.6.1.17

Molecular weight: Translated: 28270; Mature: 28270

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKVAYYAPGNHCNKLYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFIT
HHHHHHHHHEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHILDEKRLLVCHAGIRQDYIGRQDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCEEECCCCEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
KEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGRLTALRYPEMETVSVPSSLPFVPE
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCEEEEEECCCCCEEECCCCCCCCCC
KFRPIS
CCCCCC
>Mature Secondary Structure
MKYDIIGDIHGCLQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKVAYYAPGNHCNKLYRFFLGRNVTIAHGLETTVAEYEALPSHKQNMIKEKFIT
HHHHHHHHHEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHILDEKRLLVCHAGIRQDYIGRQDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCEEECCCCEEEEEECCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
KEYKGTTWIVYGHTPVKEPRFVNHTVNIDTGAVFGGRLTALRYPEMETVSVPSSLPFVPE
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCEEEEEECCCCCEEECCCCCCCCCC
KFRPIS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA