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The map label for this gene is glpF [H]

Identifier: 49183981

GI number: 49183981

Start: 1014552

End: 1015388

Strand: Direct

Name: glpF [H]

Synonym: BAS0959

Alternate gene names: 49183981

Gene position: 1014552-1015388 (Clockwise)

Preceding gene: 49183980

Following gene: 49183982

Centisome position: 19.4

GC content: 40.38

Gene sequence:

>837_bases
TTGTGGAGGGATACTATGTCAGCATTTTTAGGAGAGTTAATAGGGACTGCGTTGTTAATCGTACTTGGTGGCGGCGTTTG
TGCTGGTGTAAGTTTAAAGAAGTCGTTTGCGAAAGATTCTGGTTGGATTGTTATTACAATGGGCTGGGGCTTAGCGGTAG
CGGTTGCAGCGTACGTGGTTGGATCAATTAGTGGGGCACATTTGAATCCAGCTTTGACGATAGGATTAGCATTTAAGGGA
GCGTTCCCATGGGGTGACGTACCTGGTTATATCGCAGCACAAATGATTGGGGCAATTATCGGGGCAGTTATCGTATATTT
ACATTACTTACCACACTGGAAAGAAACAGAAGATCCAGGAACAAAGTTAGGTGTATTTGCAACAGGTCCAGCAATTCCGA
ACACATTTGCAAACCTTTTAAGTGAAATGATTGGGACATTCGTTTTAGTATTTGGTATATTAGCAATTGGTGCAAATAAA
TTTGCAGATGGATTAAATCCATTTATCGTAGGTTTCTTAATTGTAAGTATTGGTTTATCATTAGGTGGAACGACAGGATA
CGCAATCAACCCAGCACGTGATTTAGGTCCGCGTATCGCGCACTTCTTCCTTCCGATTGCAGGAAAAGGCGGTTCAAACT
GGAAGTATGCATGGATTCCAGTAGTAGGTCCGATTTTAGGTGGATCACTTGCAGGATTATTCCATCAAGTTGTATTTGAA
GGAAAACAAAATGCAGCACTTATTTATGTGATTATTGCAACTGTGATTGTATTAGCAATCTCTTATATGACAAGCAAAAA
AAGTGGAAGCAATACAAATAGTAGAAAAGTAGCATAA

Upstream 100 bases:

>100_bases
AATTTATATAGAAGCGTGCAAAGCTGTGGGTCTTTTTCTTTTGACAAAAACGAATGGCCATTCATTTTACCTCTATCACA
TTTGAAAGCGTTTAAATTGA

Downstream 100 bases:

>100_bases
GGGGGAAACCATTATGAAAAAATATATTCTTTCATTAGACCAAGGAACAACTAGCTCACGCGCAATTCTTTTCAATAAAA
AAGGTGAAATTGTTCATTCA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MWRDTMSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKG
AFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANK
FADGLNPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFE
GKQNAALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Sequences:

>Translated_278_residues
MWRDTMSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKG
AFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANK
FADGLNPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFE
GKQNAALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
>Mature_278_residues
MWRDTMSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVVGSISGAHLNPALTIGLAFKG
AFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANK
FADGLNPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFE
GKQNAALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=38.0165289256198, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI157266307, Length=257, Percent_Identity=36.5758754863813, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4502187, Length=252, Percent_Identity=35.3174603174603, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI22538420, Length=250, Percent_Identity=32, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4755125, Length=247, Percent_Identity=33.6032388663968, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502181, Length=247, Percent_Identity=33.6032388663968, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37694062, Length=251, Percent_Identity=31.0756972111554, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI310133356, Length=200, Percent_Identity=31, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310114181, Length=200, Percent_Identity=31, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=34.7490347490347, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787101, Length=189, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=230, Percent_Identity=33.4782608695652, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=34.9397590361446, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71992966, Length=234, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=262, Percent_Identity=30.5343511450382, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=262, Percent_Identity=30.5343511450382, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=28.3898305084746, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=224, Percent_Identity=29.4642857142857, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=209, Percent_Identity=36.3636363636364, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=27.1477663230241, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652747, Length=247, Percent_Identity=32.3886639676113, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45551084, Length=247, Percent_Identity=32.3886639676113, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24762342, Length=255, Percent_Identity=28.2352941176471, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24762344, Length=245, Percent_Identity=32.2448979591837, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762346, Length=245, Percent_Identity=31.4285714285714, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762348, Length=245, Percent_Identity=31.4285714285714, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130305, Length=245, Percent_Identity=31.4285714285714, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922828, Length=194, Percent_Identity=30.9278350515464, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550503, Length=244, Percent_Identity=27.0491803278689, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28921; Mature: 28921

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRDTMSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHH
GSISGAHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPG
HCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLS
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
LGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFE
CCCCCCCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
GKQNAALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MWRDTMSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYVV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHH
GSISGAHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPG
HCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLS
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
LGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFE
CCCCCCCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
GKQNAALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]