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The map label for this gene is appA [H]

Identifier: 49183882

GI number: 49183882

Start: 921953

End: 923539

Strand: Direct

Name: appA [H]

Synonym: BAS0858

Alternate gene names: 49183882

Gene position: 921953-923539 (Clockwise)

Preceding gene: 49183881

Following gene: 49183883

Centisome position: 17.63

GC content: 35.41

Gene sequence:

>1587_bases
ATGAAGAAGAAGTTGTTTGTATTACCATTTGTCCTACTATTATTTATTGCATTAGCTGCTTGTTCAGGGGAAAAAGATTC
GAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGAGA
CGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAA
GACGGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGA
TAATTTGAAATGGCATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGC
AAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAA
TTCGTTTTACCACAAGTAAGTGCATCTTTCGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGG
GGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTG
ATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAATTAGATTCTATCGTATACCGCGTTGTA
AAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAGCCACAAGACTTTAAGAA
ATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAG
ATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTT
TCTAGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAA
GTATGATAAAAAAGAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAA
CGAATAATAAAATTATGGAAAGCTTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAAT
GCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACAT
AATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAACTATGCACGTTATAAAAACGCTG
ATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTATATCATAAAATTCAAGAG
ACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATT
GAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACAATACATACGGGTAGTGGTAATTTAAAACCTATAAGAAAACTCGGTATTAATTCCGGGTTTTCTTATAGGTGTAAA
TAGTCGAGAGGGGTCGAAAT

Downstream 100 bases:

>100_bases
GAAACAAACAGTAAAGAAGCTGGTGGAAATCAGCTTCTTTAGCTTGAGGGGGGAAGTTTGTGTATAAAGTAATTGCGAAG
AGGCTTTTAAATGCAATTCC

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK

Sequences:

>Translated_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
>Mature_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=26.3598326359833, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789966, Length=493, Percent_Identity=26.5720081135903, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787762, Length=529, Percent_Identity=26.0869565217391, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789887, Length=510, Percent_Identity=24.7058823529412, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787551, Length=429, Percent_Identity=22.1445221445221, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=21.7213114754098, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787495, Length=441, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081878, Length=471, Percent_Identity=21.2314225053079, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59825; Mature: 59825

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
VRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
VRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]