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The map label for this gene is ytwF [H]

Identifier: 49183766

GI number: 49183766

Start: 796299

End: 796595

Strand: Direct

Name: ytwF [H]

Synonym: BAS0742

Alternate gene names: 49183766

Gene position: 796299-796595 (Clockwise)

Preceding gene: 49183765

Following gene: 49183767

Centisome position: 15.23

GC content: 37.04

Gene sequence:

>297_bases
ATGAAAGAAATGATAGCAAAAGAATTAGAAGAAAAATTGTTACGTAAAGAAGCAGTAAATATCGTAGACGTACGTGAAGT
AGAAGAAGTAGCTGAAGGGAAAATTCCAGAAGCGTGTAACATTCCACTAGGGCTGTTAGAATTTCGTATGCATGAGTTGG
ATAAAAAGAAAGAGTATATTATCGTTTGTCGTTCTGGCGGAAGAAGTGCAAGGGCAGTTCAATTTTTAGAAGGTTACGGT
TTTCGAGTGATTAATATGGTAGGTGGTATGTTAGCGTGGGAAGGTAAAGTAGAATAG

Upstream 100 bases:

>100_bases
TGAGAAGTAAACAAGCAGCGAAAATGTTAAAAAAATTAGGATTTCAGCATGTTATCAATGTTTCAGGCGGGATGAATGGT
TTGTGAGGAGGAAATGTAAA

Downstream 100 bases:

>100_bases
GGCGAGACGATTTTTTATAAAAATAAAAATACCCCTATAGGTAATTGGAGGGAGAACAACATGGAACAACAGAAGAAAAC
AACAATCGTTTTATTTAGCG

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE

Sequences:

>Translated_98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE
>Mature_98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11166; Mature: 11166

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IVCRSGGRSARAVQFLEGYGFRVINMVGGMLAWEGKVE
EEECCCCCCHHHHHHHHCCCCHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IVCRSGGRSARAVQFLEGYGFRVINMVGGMLAWEGKVE
EEECCCCCCHHHHHHHHCCCCHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]