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The map label for this gene is thiO [H]

Identifier: 49183720

GI number: 49183720

Start: 756142

End: 757251

Strand: Direct

Name: thiO [H]

Synonym: BAS0696

Alternate gene names: 49183720

Gene position: 756142-757251 (Clockwise)

Preceding gene: 49183719

Following gene: 49183721

Centisome position: 14.46

GC content: 39.64

Gene sequence:

>1110_bases
ATGTGTAAGAAGTATGATGTAGCGATAATTGGCGGTGGTGTAATTGGTAGTTCAGTTGCACATTTTCTAGCAGAAAGAGG
CCATAAAGTAGCGATTGTAGAGAAGCAACAGATTGCATCTGAAGCCTCGAAAGCAGCCGCTGGTTTACTTGGGGTGCAGG
CAGAATGGGATGAATATGACCCGCTATTTGACCTTGCTAGAGAAAGCCGTGCTATATTTCCACAACTTGCAGAAGTTTTA
CGTGAAAAAACAGGCATTGATATTGGGTATGAAGAGAAAGGAATTTATCGCATTGCTCAAAATGAAGATGAGAAGGAAAG
AATTCTTCACATTATGGATTGGCAACAGAAAACAGGTGAAGATTCTTACTTTCTAACAGGAGATCGTTTACGAGAGCAAG
AGCCGTATCTATCTGAGTCAATTATAGGTGCTGTATATTATCCAAAAGATGGCCATGTTATTGCGCCAGAGCTTACGAAA
GCATTCGCGCATTCTGCGGCAATTTCCGGTGCAGATATATATGAGCAAACAGAAGTGTTTGATATTCGTATTGAAAATAA
GAAAGTGATTGGGATTGTTACAAGTGAAGGTATGATCTCGTGCGAGAAAGTTGTTATTGCCGGTGGTTCATGGAGCACGA
AGTTACTAGGTTATTTTCACCGCGAATGGGGTACATATCCAGTTAAAGGAGAAGTAGTAGCAGTAAAAAGTAGAAAACAA
CTTTTAAAAGCACCTATTTTTCAAGAAAGATTTTACATTGCCCCAAAACGCGGCGGACGTTACGTAATTGGAGCAACGAT
GAAGCCACATACGTTCAATAAAACTGTGCAACCAGAAAGTATTACTTCTATATTAGAGCGTGCTTATACAATATTACCAG
CTTTAAAAGAAGCAGAATGGGAAAGTACGTGGGCAGGGCTAAGACCACAATCGAATCATGAAGCTCCTTATATGGGAGAG
CATGAAGAAATAAAAGGTTTATATGCTTGCACGGGCCATTATCGAAACGGTATTTTATTAAGTCCTGTTTCTGGTCAATA
TATGGCTGATTTAATAGAAGGAAAGCAAGAGAATCACTTGCTAGATTCATTGCTTTCTAAAACGGTTTAG

Upstream 100 bases:

>100_bases
GTAAGTGGTATTGCTGTTATGTCTGGAATTGTAAGTAGTAGTAACCCGTATAGCAAAGCGAAATCTTATAAGGAATCAAT
AAGAAAGTGGGCGGAAAAAC

Downstream 100 bases:

>100_bases
AAAGGGGATGGAAGTTTGAATTTGAAAATTAATGGTAATCAAATTGAAGTGCCAGAGAGTGTAAAAACAGTAGCCGAGCT
ACTTACACATTTAGAGTTAG

Product: glycine oxidase

Products: NA

Alternate protein names: GO [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDEYDPLFDLARESRAIFPQLAEVL
REKTGIDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV

Sequences:

>Translated_369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDEYDPLFDLARESRAIFPQLAEVL
REKTGIDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV
>Mature_369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDEYDPLFDLARESRAIFPQLAEVL
REKTGIDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV

Specific function: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize th

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DAO family [H]

Homologues:

Organism=Homo sapiens, GI24797151, Length=382, Percent_Identity=24.3455497382199, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI194306651, Length=211, Percent_Identity=27.0142180094787, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=22.7154046997389, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=22.7154046997389, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1787438, Length=404, Percent_Identity=24.7524752475248, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787559, Length=383, Percent_Identity=22.1932114882507, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32563613, Length=389, Percent_Identity=24.4215938303342, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=396, Percent_Identity=22.979797979798, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994052, Length=403, Percent_Identity=22.5806451612903, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20130091, Length=364, Percent_Identity=25.8241758241758, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.3.19 [H]

Molecular weight: Translated: 41254; Mature: 41254

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDEYD
CCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEECCCCCCCCC
PLFDLARESRAIFPQLAEVLREKTGIDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHCCC
DSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCHHHHCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
EEEECCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEW
HHHCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSKTV
HHHHHHHCC
>Mature Secondary Structure
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDEYD
CCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEECCCCCCCCC
PLFDLARESRAIFPQLAEVLREKTGIDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHCCC
DSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCHHHHCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
EEEECCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEW
HHHCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSKTV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9827558; 11744710 [H]