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The map label for this gene is sph [H]

Identifier: 49183669

GI number: 49183669

Start: 695830

End: 696966

Strand: Direct

Name: sph [H]

Synonym: BAS0644

Alternate gene names: 49183669

Gene position: 695830-696966 (Clockwise)

Preceding gene: 49183668

Following gene: 49183670

Centisome position: 13.31

GC content: 36.15

Gene sequence:

>1137_bases
ATGCACAACGTGTTACTGCTGGATACATTCAGCTTTGGTTTGATACGTACGGAGATCGTTAAGGATTTAAAAAAGGTCAA
ATCTCACAATAGAGTATTTGACCTTTTTATTACTATACACATGGAGGTATGGAACGTGAAAGGTAAATTGCTAAAAGGTG
TACTTAGCTTTGGGATTGGTTTAGGAGTTTTATACGGAGGATCCTCAGCTCAAGCAGATACGTCTACAGATCAAAACAAT
ACTTTGAAAGTGATGACGCATAACGTGTACATGCTATCAACTAACTTATATCCGAATTGGGGACAAAGTGAGCGTGCTGA
TTTAATTGGGGCGGCAGATTATATTAAGAATCAAGACGTTGTTATATTAAATGAAGTGTTTGATAATAGTGCTTCAGATC
GCCTTTTAGGAAATTTGAAGAAAGAATACCCAAATCAAACAGCTGTATTAGGTCGTAGCAACGGAAATGAATGGGATAAA
ACGTTAGGTAGCTATTCATCTGCAACTCCTGAAGATGGAGGCGTTGCAATCGTTAGCAAATGGCCGATCGTTGAAAAGGT
TCAATATGTATTTGCAAATGGATGTGGACCAGATAATTTATCGAATAAAGGATTTGTATACACGAAAATTAAGAAAAATG
ATCGTTTCGTTCATGTAATTGGGACGCATTTGCAGGCTGAAGATAGTATGTGCGGAAAAACTTCACCTGCATCTGTACGT
ACAAACCAGTTAAAAGAAATTCAAGATTTTATTAAAAATAAAAATATACCAAATGACGAGTACGTCCTATTTGGTGGTGA
TATGAATGTGAATAAAATAAATGCTGAGAACAATAGTGACTCAGAATACGCATCAATGTTCAAAACATTGCACGCTTCTA
TTCCATCTTATACAGGACATACAGCAACTTGGGATGCAACGACAAACAGTATTGCAAAATATAATTTCCCTGATAGCCCT
GCCGAGTATTTAGATTATATTATTGCAAGTAAGGACCATGCGAATCCGTCATTTATAGAGAATAAAGTATTACAACCGAA
ATCTCCACAGTGGACTGTTACATCATGGCTCAAAAAATATACATATAATGATTATTCTGATCATTATCCAGTCGAGGCGA
CTATTTCTATGAAGTAG

Upstream 100 bases:

>100_bases
GATAATACGAAAGATTGGTTCGTAAAAGCAGCTGTGTCACAAGAATATGCAGATAAATGGCGTGCTGAAGTTACACCGAT
GACAGGTAAGCGATTAATGG

Downstream 100 bases:

>100_bases
TCTTAAAAAGTTTCTTCTTAATAGGAAGAAACTTTTTTATTTTGTAGAGGAAATTTCAACTTTCTTTCCAATATGTATAA
GACATATTGAAAAGAGGAGA

Product: sphingomyelinase C

Products: NA

Alternate protein names: SMase; Cereolysin B; SMPLC; Sphingomyelin phosphodiesterase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MHNVLLLDTFSFGLIRTEIVKDLKKVKSHNRVFDLFITIHMEVWNVKGKLLKGVLSFGIGLGVLYGGSSAQADTSTDQNN
TLKVMTHNVYMLSTNLYPNWGQSERADLIGAADYIKNQDVVILNEVFDNSASDRLLGNLKKEYPNQTAVLGRSNGNEWDK
TLGSYSSATPEDGGVAIVSKWPIVEKVQYVFANGCGPDNLSNKGFVYTKIKKNDRFVHVIGTHLQAEDSMCGKTSPASVR
TNQLKEIQDFIKNKNIPNDEYVLFGGDMNVNKINAENNSDSEYASMFKTLHASIPSYTGHTATWDATTNSIAKYNFPDSP
AEYLDYIIASKDHANPSFIENKVLQPKSPQWTVTSWLKKYTYNDYSDHYPVEATISMK

Sequences:

>Translated_378_residues
MHNVLLLDTFSFGLIRTEIVKDLKKVKSHNRVFDLFITIHMEVWNVKGKLLKGVLSFGIGLGVLYGGSSAQADTSTDQNN
TLKVMTHNVYMLSTNLYPNWGQSERADLIGAADYIKNQDVVILNEVFDNSASDRLLGNLKKEYPNQTAVLGRSNGNEWDK
TLGSYSSATPEDGGVAIVSKWPIVEKVQYVFANGCGPDNLSNKGFVYTKIKKNDRFVHVIGTHLQAEDSMCGKTSPASVR
TNQLKEIQDFIKNKNIPNDEYVLFGGDMNVNKINAENNSDSEYASMFKTLHASIPSYTGHTATWDATTNSIAKYNFPDSP
AEYLDYIIASKDHANPSFIENKVLQPKSPQWTVTSWLKKYTYNDYSDHYPVEATISMK
>Mature_378_residues
MHNVLLLDTFSFGLIRTEIVKDLKKVKSHNRVFDLFITIHMEVWNVKGKLLKGVLSFGIGLGVLYGGSSAQADTSTDQNN
TLKVMTHNVYMLSTNLYPNWGQSERADLIGAADYIKNQDVVILNEVFDNSASDRLLGNLKKEYPNQTAVLGRSNGNEWDK
TLGSYSSATPEDGGVAIVSKWPIVEKVQYVFANGCGPDNLSNKGFVYTKIKKNDRFVHVIGTHLQAEDSMCGKTSPASVR
TNQLKEIQDFIKNKNIPNDEYVLFGGDMNVNKINAENNSDSEYASMFKTLHASIPSYTGHTATWDATTNSIAKYNFPDSP
AEYLDYIIASKDHANPSFIENKVLQPKSPQWTVTSWLKKYTYNDYSDHYPVEATISMK

Specific function: Required, with sphingomyelinase, to effect target cell lysis (hemolysis) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005135
- InterPro:   IPR017766 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.4.12 [H]

Molecular weight: Translated: 42285; Mature: 42285

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNVLLLDTFSFGLIRTEIVKDLKKVKSHNRVFDLFITIHMEVWNVKGKLLKGVLSFGIG
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHH
LGVLYGGSSAQADTSTDQNNTLKVMTHNVYMLSTNLYPNWGQSERADLIGAADYIKNQDV
EEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCE
VILNEVFDNSASDRLLGNLKKEYPNQTAVLGRSNGNEWDKTLGSYSSATPEDGGVAIVSK
EEEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEC
WPIVEKVQYVFANGCGPDNLSNKGFVYTKIKKNDRFVHVIGTHLQAEDSMCGKTSPASVR
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEECCCCCCCCCCCCCHH
TNQLKEIQDFIKNKNIPNDEYVLFGGDMNVNKINAENNSDSEYASMFKTLHASIPSYTGH
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
TATWDATTNSIAKYNFPDSPAEYLDYIIASKDHANPSFIENKVLQPKSPQWTVTSWLKKY
EEEECCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHH
TYNDYSDHYPVEATISMK
CCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MHNVLLLDTFSFGLIRTEIVKDLKKVKSHNRVFDLFITIHMEVWNVKGKLLKGVLSFGIG
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHH
LGVLYGGSSAQADTSTDQNNTLKVMTHNVYMLSTNLYPNWGQSERADLIGAADYIKNQDV
EEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCE
VILNEVFDNSASDRLLGNLKKEYPNQTAVLGRSNGNEWDKTLGSYSSATPEDGGVAIVSK
EEEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEC
WPIVEKVQYVFANGCGPDNLSNKGFVYTKIKKNDRFVHVIGTHLQAEDSMCGKTSPASVR
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEECCCCCCCCCCCCCHH
TNQLKEIQDFIKNKNIPNDEYVLFGGDMNVNKINAENNSDSEYASMFKTLHASIPSYTGH
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
TATWDATTNSIAKYNFPDSPAEYLDYIIASKDHANPSFIENKVLQPKSPQWTVTSWLKKY
EEEECCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHH
TYNDYSDHYPVEATISMK
CCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2848222; 8387306; 3137122 [H]