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The map label for this gene is cadA [H]

Identifier: 49183589

GI number: 49183589

Start: 607829

End: 610195

Strand: Direct

Name: cadA [H]

Synonym: BAS0564

Alternate gene names: 49183589

Gene position: 607829-610195 (Clockwise)

Preceding gene: 49183588

Following gene: 49183591

Centisome position: 11.62

GC content: 36.33

Gene sequence:

>2367_bases
ATGGCTGAAGCATTAGTGAAAAAGAAACTGATGTTAGAAGGTTTAGATTGTGCGAACTGTGCAATGAAAATTGAAAAAGG
CGTTGGAAATATAGAAGGAGTAAATTCTTGCTCTGTAAATTTCGCGACAAAAACAATGGTTTTAGAAACAGCGCAAAATA
AAGAAAGTGAAGTTGTTACAGAAGCAAAAAAACTCGTTACAAAATTAGAACCGCATATTAAAGTGCAAGAAGAAAAAAAC
ACAAAAGTAGCAAAAGAAGTGTTTATATTAGAAGGTTTAGATTGCGCGAACTGTGCGATGAAGATTGAAAATAAAGTTAA
GGAAATGCCGACTGTTTCAGCAGCAACTGTTGATTTCGTATCAAAGAAATTACGAGTTGAAGTAGCAAATAAAAGAGAAT
TAGAAGCGACTGTAGCGAATATAACAAATGTCGTTCAAAAGTTAGAACCAGATGTGAAAGTTGTTCGTGAAGAAAAGGGT
GGTCATGACCACGGACATAGTCACGATCATGGTGAAGCAAATGTGAAAAAGATGGTAGGCCGATTAGTGGTCGGCGGAAT
TTTGACAGCAATTGCTGCATTAGCTGGCTTACCACAAATGATAACAATTCCGTTATTCGTCCTTGCATATTTATTAATCG
GTGGAGATATCGTTTGGAGAGCGGTAAGAAACATAACTCGTGGCCAAGTGTTCGATGAAAACTTCTTAATGGCAATTGCA
ACGCTAGGAGCTTTTGCGATTCAGCAATATCCGGAAGCAGTTGCAGTAATGTTATTTTATCAAGTAGGGGAACTATTCCA
AAGTATTGCGGTAAACCGTTCTCGAAAATCAATTACTTCATTAATGGATATTCGTCCTGATTACGCGAATGTAAAGATTG
GAAATGAAACGAAACAAGTATCACCAGAAGATGTACAAATCGGCGATTATATTATCGTGAAACCTGGTGAGAAAGTACCA
TTAGACGGAAAAGTAGTTGAAGGAACATCAATGGTAGATACTTCAGCATTGACTGGTGAATCTGTACCACGTGAAGTTGA
AGTTGGAAATGATGTACTAAGTGGTTTTGTGAACCAAAATGGTGTATTAACAATTGAAGTTACAAAAGAATTTGGTGAAT
CAACTGTATCAAAAATTTTAGACTTAGTTCAAAATGCAAGCAGTAAAAAAGCGCCAACAGAAAACTTTATTACGAAGTTC
GCGCGTTACTACACGCCAGTTGTAGTAATTACAGCAGCGATCATGGCGTTTATTCCACCACTTATTTTAGAAGGAGCTAC
ATTCTCTGATTGGATTTATAGAGCTCTAGTGTTCTTAGTAATCTCTTGTCCATGTGCGTTAGTTGTATCTATTCCTCTTG
GATTCTTTGGAGGTATCGGTGGTGCATCTAAAAGTGGTGTGTTAGTAAAAGGAAGTAACTATTTAGAAGCTTTAAATGAT
GTGAAATATATTGTTTTTGATAAAACAGGAACATTAACAAAAGGTGTCTTCAAAGTTACAAAAATGGAACCGAGCGAAGG
CATTACAAATGAAGAATTATTAGAGTATGCAGCATTTGCTGAAGTATATTCTAACCATCCAATTGCACAATCGATTCGAA
ATGCATACCGAAAATCAATTGATGAAAAAATAATTGATGATTATAACGAAATTTCTGGTCACGGTACAGTCGTAAAGGTA
CAAGGGAAAGAAATTTTTGCAGGTAATGCTAAATTAATGAGAAAAGAAAACATTACATTTAAGCAACCAGAAACAGTAGG
TACATTAGTTCACGTTGCTGTAGATGGAAAATATGCAGGTTATATTGTTATCTCTGATGAAGTAAAAGAAGATTCAAAAC
AAGCAATTCAAAAGTTAAAAGAACTAGGTATTAAGAAGACAGTTATGTTAACTGGTGATGCAAAACTAGTTGGTGAAGCT
GTTGGGAAAGAATTAGGTTTAGATGAAGTTCACGCAGAACTATTACCGCAACAAAAAGTAGAAGAAATTGAAAAAATTGA
TGCAGCGAAACATGGAAAAGAAAAAGTTGCTTTCGTTGGTGATGGTATTAATGACACACCGGTATTAGCAAGAGCGGATG
TTGGTATTGCAATGGGTGGTTTAGGTTCAGATGCAGCAATTGAAGCAGCAGACATCGTTATTATGACTGATGAACCTTCA
AAGATTGCAACAGCTGTAAACATTGCAAAACGTACACGACGCATCGTATGGCAAAATATCATCTTTGCATTAGGGGTAAA
AGGTTTAGTTTTACTACTTGGTGCCTTCGGTATTGCAACAATGTGGGAAGCGGTCTTCTCAGATGTTGGTGTGACACTAC
TGGCAGTGTTAAATGCAATGCGTGTACTACGAGTGAAAGATTTATAA

Upstream 100 bases:

>100_bases
TTATTATTCGTTAGCTGACCAACACGTCATTCATATCTTCGAGCAAGCGTTTGAACACGTAAACGAGGAAGAATAAAAAA
GAACGCGAGGAGGGAGAACG

Downstream 100 bases:

>100_bases
AAAGAGAGGACGCAACCCGCGTCCTCTCTTTTTAGGTTATTCTGTTGAAATTGACTCAATAAAATAATAACAAATATTAT
TTTATTGATGAATAACGTAA

Product: heavy metal-transporting ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVTEAKKLVTKLEPHIKVQEEKN
TKVAKEVFILEGLDCANCAMKIENKVKEMPTVSAATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKG
GHDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMITIPLFVLAYLLIGGDIVWRAVRNITRGQVFDENFLMAIA
TLGAFAIQQYPEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKIGNETKQVSPEDVQIGDYIIVKPGEKVP
LDGKVVEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKF
ARYYTPVVVITAAIMAFIPPLILEGATFSDWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
VKYIVFDKTGTLTKGVFKVTKMEPSEGITNEELLEYAAFAEVYSNHPIAQSIRNAYRKSIDEKIIDDYNEISGHGTVVKV
QGKEIFAGNAKLMRKENITFKQPETVGTLVHVAVDGKYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKLVGEA
VGKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKVAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
KIATAVNIAKRTRRIVWQNIIFALGVKGLVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL

Sequences:

>Translated_788_residues
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVTEAKKLVTKLEPHIKVQEEKN
TKVAKEVFILEGLDCANCAMKIENKVKEMPTVSAATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKG
GHDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMITIPLFVLAYLLIGGDIVWRAVRNITRGQVFDENFLMAIA
TLGAFAIQQYPEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKIGNETKQVSPEDVQIGDYIIVKPGEKVP
LDGKVVEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKF
ARYYTPVVVITAAIMAFIPPLILEGATFSDWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
VKYIVFDKTGTLTKGVFKVTKMEPSEGITNEELLEYAAFAEVYSNHPIAQSIRNAYRKSIDEKIIDDYNEISGHGTVVKV
QGKEIFAGNAKLMRKENITFKQPETVGTLVHVAVDGKYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKLVGEA
VGKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKVAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
KIATAVNIAKRTRRIVWQNIIFALGVKGLVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL
>Mature_787_residues
AEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVTEAKKLVTKLEPHIKVQEEKNT
KVAKEVFILEGLDCANCAMKIENKVKEMPTVSAATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKGG
HDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMITIPLFVLAYLLIGGDIVWRAVRNITRGQVFDENFLMAIAT
LGAFAIQQYPEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKIGNETKQVSPEDVQIGDYIIVKPGEKVPL
DGKVVEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFA
RYYTPVVVITAAIMAFIPPLILEGATFSDWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALNDV
KYIVFDKTGTLTKGVFKVTKMEPSEGITNEELLEYAAFAEVYSNHPIAQSIRNAYRKSIDEKIIDDYNEISGHGTVVKVQ
GKEIFAGNAKLMRKENITFKQPETVGTLVHVAVDGKYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKLVGEAV
GKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKVAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPSK
IATAVNIAKRTRRIVWQNIIFALGVKGLVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL

Specific function: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=869, Percent_Identity=25.0863060989643, Blast_Score=257, Evalue=3e-68,
Organism=Homo sapiens, GI55743073, Length=543, Percent_Identity=28.5451197053407, Blast_Score=223, Evalue=7e-58,
Organism=Homo sapiens, GI115529486, Length=904, Percent_Identity=25.4424778761062, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI118498343, Length=212, Percent_Identity=28.7735849056604, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789879, Length=716, Percent_Identity=33.7988826815642, Blast_Score=356, Evalue=3e-99,
Organism=Escherichia coli, GI1786691, Length=855, Percent_Identity=27.4853801169591, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1786914, Length=475, Percent_Identity=29.4736842105263, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI2367363, Length=592, Percent_Identity=24.8310810810811, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556548, Length=883, Percent_Identity=27.0668176670442, Blast_Score=221, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320475, Length=561, Percent_Identity=30.3030303030303, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319772, Length=635, Percent_Identity=24.7244094488189, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321271, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221329854, Length=612, Percent_Identity=27.2875816993464, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24668704, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24668708, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281366617, Length=153, Percent_Identity=27.4509803921569, Blast_Score=71, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24668696, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161085803, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281366676, Length=293, Percent_Identity=25.938566552901, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3; =3.6.3.5 [H]

Molecular weight: Translated: 85349; Mature: 85218

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVT
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHEEEHHCCCHHHHHH
EAKKLVTKLEPHIKVQEEKNTKVAKEVFILEGLDCANCAMKIENKVKEMPTVSAATVDFV
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKGGHDHGHSHDHGEANVKKMVG
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RLVVGGILTAIAALAGLPQMITIPLFVLAYLLIGGDIVWRAVRNITRGQVFDENFLMAIA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHH
TLGAFAIQQYPEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKIGNETKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCCC
SPEDVQIGDYIIVKPGEKVPLDGKVVEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQN
CCCCEEECCEEEECCCCCCCCCCEEECCCCCCCCHHCCCCCCCCHHHCCHHHHHHHCCCC
GVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFARYYTPVVVITAAIMAFIPP
CEEEEEEHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILEGATFSDWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCC
VKYIVFDKTGTLTKGVFKVTKMEPSEGITNEELLEYAAFAEVYSNHPIAQSIRNAYRKSI
CEEEEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEKIIDDYNEISGHGTVVKVQGKEIFAGNAKLMRKENITFKQPETVGTLVHVAVDGKYAG
HHHHHHHHHHCCCCCCEEEEECCEEEECCHHHHEECCCCCCCCCCCCEEEEEEECCCEEE
YIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKLVGEAVGKELGLDEVHAELLPQQKV
EEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHCCHHHH
EEIEKIDAAKHGKEKVAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCCEEEEEEEEECCCCH
KIATAVNIAKRTRRIVWQNIIFALGVKGLVLLLGAFGIATMWEAVFSDVGVTLLAVLNAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLRVKDL
HHHHHCCC
>Mature Secondary Structure 
AEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVT
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHEEEHHCCCHHHHHH
EAKKLVTKLEPHIKVQEEKNTKVAKEVFILEGLDCANCAMKIENKVKEMPTVSAATVDFV
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKGGHDHGHSHDHGEANVKKMVG
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RLVVGGILTAIAALAGLPQMITIPLFVLAYLLIGGDIVWRAVRNITRGQVFDENFLMAIA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHH
TLGAFAIQQYPEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKIGNETKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCCC
SPEDVQIGDYIIVKPGEKVPLDGKVVEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQN
CCCCEEECCEEEECCCCCCCCCCEEECCCCCCCCHHCCCCCCCCHHHCCHHHHHHHCCCC
GVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFARYYTPVVVITAAIMAFIPP
CEEEEEEHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILEGATFSDWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCC
VKYIVFDKTGTLTKGVFKVTKMEPSEGITNEELLEYAAFAEVYSNHPIAQSIRNAYRKSI
CEEEEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEKIIDDYNEISGHGTVVKVQGKEIFAGNAKLMRKENITFKQPETVGTLVHVAVDGKYAG
HHHHHHHHHHCCCCCCEEEEECCEEEECCHHHHEECCCCCCCCCCCCEEEEEEECCCEEE
YIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKLVGEAVGKELGLDEVHAELLPQQKV
EEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHCCHHHH
EEIEKIDAAKHGKEKVAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCCEEEEEEEEECCCCH
KIATAVNIAKRTRRIVWQNIIFALGVKGLVLLLGAFGIATMWEAVFSDVGVTLLAVLNAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLRVKDL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]