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The map label for this gene is miaB

Identifier: 46447392

GI number: 46447392

Start: 2101062

End: 2102414

Strand: Direct

Name: miaB

Synonym: pc1758

Alternate gene names: 46447392

Gene position: 2101062-2102414 (Clockwise)

Preceding gene: 46447389

Following gene: 46447403

Centisome position: 87.02

GC content: 36.07

Gene sequence:

>1353_bases
ATGTTCAGCTTTAATCTTTTAACCCAATACATAATTATGCGATCATTAAAAAAGTTTTTTGTCAAAACTTACGGCTGTCA
AATGAATGAACTCGATTCAGAAATTATGATTGGCCAATTAGAAAATCGAGGGCTGACTCGTAGTCATGATGAAAATGACG
CCGATCTTTTGATTTTTAACACTTGCTCTATCCGAGATCTCGCAGAAAGAAAAGTCATGGGCAAATTAGGAAAATTAGGA
TTAACTAAACAAAGTCAAGCAATTATTGGCGTAACCGGGTGTATGGCTAATGCTAAAAAAGACTCCCTATTCCAAAAACT
TCCTCATATTGACTTTGTTCTTGGTACTAACAATATCCATGATTTAAACCATGTACTTGATGAAGTTTTAGCTTCAGGTA
AGCAATCTATTCGAACAGATGATCATTTTGAATTCGAACTCGATTACTTGAATGCAAAAAGAGAAGATCAAATTAAAGCT
TATGTTTCGATTATCCGTGGATGTGATAAATTTTGTACTTATTGCGTTGTTCCGTATACAAGAGGTTCAGAGGTTTCTCG
AGCTCCTGAAAATATTTTAGAAGAGTGTCGTCACCTTGTCAATCAAGGCTATAAAGAAATAACTTTACTTGGACAAAATG
TCAATAGCTACGGAAAAGACAAACTTGAATGGAAATGCCTTTTCCATGATTTGCTTTATCAATTAGATAAAATTCCAGGA
TTAGAAAGAGTCAGATTTATGACAAGCCACCCTGTTGATATTTCGAAAGAGCTCATGGAAGCTATTCGAGATCTTAAAAC
GCTATGCGAATTTGTTCACTTTCCATTACAAGCTGGTTCTAATCGCGTCCTAAAAAAAATGCATCGCATTTATACCGTTG
AACAATATTTAGAAAAAGTTCAAATGCTTAAAGAAATTGTTCCCAATGTTGCACTTGGAACGGATATTATTGTTGGTTTC
CCAACCGAAACGGAAGAAGAATTTCAAGAAACTTACCGTTTACTCAAAGAAATTGAATACTCCGTTGCTTTCTTGTTTTC
ATATAGCCCAAGGAAAGGAACTCCAGCCATGCGATGGCGTGATGATGTCCCTGAAGAAGTCAAGCAAGACCGTTTGCAAA
GGCTTTTACAACTCCAAGATACTATTTATATGAAACATAGACAGGCGTTTTTAGGGCAAACAGTAGAAGTTCTTGTAGAA
AGACGAAACTTTAAAGATGATCGCCTTGTCAAAGGTCGCACGCGTTGCTGGAAAAACGTTTTGTTTACAGGTGGAGATGA
GTTGGTTGGAACAATGCAGCAAGTTAAAATTCATGGCTATAGCCATCAAACTTTGTTAGGTGATTTACAGTAA

Upstream 100 bases:

>100_bases
TGAAATAAAGATCTTTTATCATTAAAATTCTTTCTCTCTGCGAATTTTTTTGGCTGGTTTTTTTGAAATAGCGGGTTTGG
CTCGATTCAAAAGCCTTTAA

Downstream 100 bases:

>100_bases
CTGACTGATTTAAAAAAGTGGGTGATATAAATACGAATAACCCACTTTTTTAAATCAGCCTTAATGGTTATTCATTTTCG
ATTTCAATTGTTCCGCAACC

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFNTCSIRDLAERKVMGKLGKLG
LTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIHDLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKA
YVSIIRGCDKFCTYCVVPYTRGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG
LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKVQMLKEIVPNVALGTDIIVGF
PTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWRDDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVE
RRNFKDDRLVKGRTRCWKNVLFTGGDELVGTMQQVKIHGYSHQTLLGDLQ

Sequences:

>Translated_450_residues
MFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFNTCSIRDLAERKVMGKLGKLG
LTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIHDLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKA
YVSIIRGCDKFCTYCVVPYTRGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG
LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKVQMLKEIVPNVALGTDIIVGF
PTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWRDDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVE
RRNFKDDRLVKGRTRCWKNVLFTGGDELVGTMQQVKIHGYSHQTLLGDLQ
>Mature_450_residues
MFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFNTCSIRDLAERKVMGKLGKLG
LTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIHDLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKA
YVSIIRGCDKFCTYCVVPYTRGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG
LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKVQMLKEIVPNVALGTDIIVGF
PTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWRDDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVE
RRNFKDDRLVKGRTRCWKNVLFTGGDELVGTMQQVKIHGYSHQTLLGDLQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=35.0409836065574, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=35.2201257861635, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI93277076, Length=439, Percent_Identity=28.0182232346241, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1786882, Length=437, Percent_Identity=38.6727688787185, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787057, Length=395, Percent_Identity=32.1518987341772, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=421, Percent_Identity=36.3420427553444, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=273, Percent_Identity=30.7692307692308, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=34.7280334728033, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=30.3964757709251, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PARUW (Q6MAB7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008757.1
- ProteinModelPortal:   Q6MAB7
- SMR:   Q6MAB7
- STRING:   Q6MAB7
- GeneID:   2781015
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1758
- NMPDR:   fig|264201.1.peg.1758
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   CLSK2762486
- BioCyc:   CPRO264201:PC1758-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52228; Mature: 52228

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFN
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEEE
TCSIRDLAERKVMGKLGKLGLTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIH
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHCCCCEEEECCCCHH
DLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKAYVSIIRGCDKFCTYCVVPYT
HHHHHHHHHHHCCHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCC
RGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG
CCCCHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QMLKEIVPNVALGTDIIVGFPTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWR
HHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
DDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVERRNFKDDRLVKGRTRCWKNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LFTGGDELVGTMQQVKIHGYSHQTLLGDLQ
HHCCCHHHHHHHHHHHCCCCCCHHHCCCCC
>Mature Secondary Structure
MFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFN
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEEE
TCSIRDLAERKVMGKLGKLGLTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIH
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHCCCCEEEECCCCHH
DLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKAYVSIIRGCDKFCTYCVVPYT
HHHHHHHHHHHCCHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCC
RGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG
CCCCHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QMLKEIVPNVALGTDIIVGFPTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWR
HHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
DDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVERRNFKDDRLVKGRTRCWKNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LFTGGDELVGTMQQVKIHGYSHQTLLGDLQ
HHCCCHHHHHHHHHHHCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA