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The map label for this gene is folD

Identifier: 46447335

GI number: 46447335

Start: 2039368

End: 2040246

Strand: Direct

Name: folD

Synonym: pc1701

Alternate gene names: 46447335

Gene position: 2039368-2040246 (Clockwise)

Preceding gene: 46447333

Following gene: 46447336

Centisome position: 84.46

GC content: 38.34

Gene sequence:

>879_bases
ATGATTATTGATGGAAAGCGAATAGCAGAAGAAATTCAACTGGAAATCAAAGAACTCATCCAACAATCTCTCTGTCGCCC
TCCTTGCTTAGCTGTTTTAATTGTGGGTAGCCATCCTGCCTCTCAAATCTATGTCAATCGTAAAACTGCAGCTTGTAAAG
CGGTTGGTATTGCTTCTATCAAGTGTGAATTACCTTTCACGATTTCTGAAGAAGAATTAATTCGAGAAGTCGAAAAGCTC
AACGTTGATCCCCAAATTGATGGAATTCTTGTTCAACTTCCTCTACCTCCTCACATCAACTCTAATCGGGTTAATTACCA
TATTGATCCTCAAAAAGATGTCGATGGTTTTCATCCTCTCAATGTTGGAAAAATGCTGATTGGTGAATTAGATGGTTTTT
TACCCTGCACTCCTTTCGGCATTAAAACATTGTTAGAACGCACTGGCATTGAAGTTTGTGGGAAACATGCTCTGATTATT
GGACGAAGCAATATTGTGGGTAAACCAATGGCAGCTTTGTTAATGCAATCATATCCTGGAGGAAATGCAACCGTTACAGT
AGCTCATCGATATACCAAAAATCTCAAAGAACTCTGTTTACAAGCGGACCTTATTATCGTAGCCATAGGCCAACCTAAAT
TGATTACAGCAGATATGGTCAAAGAGGGGGTCATTATTGTCGATGTAGGTATCAATAAAATTCATGATTCTTCCAAAAAA
AATGGTTACCAAATTGTAGGAGATGTCGATTTTGTTAATGTAGCTCCCAAGTGTGCTTTTATCACACCAGTACCCGGAGG
TGTAGGACCAATGACAATCGCTATGCTTTTATATAATACTCTATTAAGCTATTCGAAATCGCAGAATTTAAACTTGTGA

Upstream 100 bases:

>100_bases
TTTCACGAGATAAATAAAGCATTAAAATTTAACTTCGACTCTTTTGAGGTTAATAAAAACTTGCTATAATAATCTACTTG
AAAAGAAACGAGGAAATGAA

Downstream 100 bases:

>100_bases
ATACAATCCATTTATTTACTTATATTCGCTCCTTGCAAACTTTTACTGCCAAATTTCGCTCTTTATTTATGCTTATTTGG
ATGAGTTTTAGTTTTCTTTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/methenyl tetrahydrofolate cyclohydrolase FolD

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIIDGKRIAEEIQLEIKELIQQSLCRPPCLAVLIVGSHPASQIYVNRKTAACKAVGIASIKCELPFTISEEELIREVEKL
NVDPQIDGILVQLPLPPHINSNRVNYHIDPQKDVDGFHPLNVGKMLIGELDGFLPCTPFGIKTLLERTGIEVCGKHALII
GRSNIVGKPMAALLMQSYPGGNATVTVAHRYTKNLKELCLQADLIIVAIGQPKLITADMVKEGVIIVDVGINKIHDSSKK
NGYQIVGDVDFVNVAPKCAFITPVPGGVGPMTIAMLLYNTLLSYSKSQNLNL

Sequences:

>Translated_292_residues
MIIDGKRIAEEIQLEIKELIQQSLCRPPCLAVLIVGSHPASQIYVNRKTAACKAVGIASIKCELPFTISEEELIREVEKL
NVDPQIDGILVQLPLPPHINSNRVNYHIDPQKDVDGFHPLNVGKMLIGELDGFLPCTPFGIKTLLERTGIEVCGKHALII
GRSNIVGKPMAALLMQSYPGGNATVTVAHRYTKNLKELCLQADLIIVAIGQPKLITADMVKEGVIIVDVGINKIHDSSKK
NGYQIVGDVDFVNVAPKCAFITPVPGGVGPMTIAMLLYNTLLSYSKSQNLNL
>Mature_292_residues
MIIDGKRIAEEIQLEIKELIQQSLCRPPCLAVLIVGSHPASQIYVNRKTAACKAVGIASIKCELPFTISEEELIREVEKL
NVDPQIDGILVQLPLPPHINSNRVNYHIDPQKDVDGFHPLNVGKMLIGELDGFLPCTPFGIKTLLERTGIEVCGKHALII
GRSNIVGKPMAALLMQSYPGGNATVTVAHRYTKNLKELCLQADLIIVAIGQPKLITADMVKEGVIIVDVGINKIHDSSKK
NGYQIVGDVDFVNVAPKCAFITPVPGGVGPMTIAMLLYNTLLSYSKSQNLNL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=46.7796610169492, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=47.7966101694915, Blast_Score=256, Evalue=1e-68,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=41.7808219178082, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI36796743, Length=202, Percent_Identity=26.7326732673267, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=50.8833922261484, Blast_Score=263, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=297, Percent_Identity=39.3939393939394, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=43.7931034482759, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=39.3333333333333, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=320, Percent_Identity=22.5, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.9387755102041, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=46.7796610169492, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI62472483, Length=283, Percent_Identity=42.0494699646643, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI45551871, Length=283, Percent_Identity=42.0494699646643, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24645718, Length=283, Percent_Identity=42.0494699646643, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI17137370, Length=283, Percent_Identity=42.0494699646643, Blast_Score=240, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PARUW (Q6MAH4)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008700.1
- HSSP:   P24186
- ProteinModelPortal:   Q6MAH4
- SMR:   Q6MAH4
- STRING:   Q6MAH4
- GeneID:   2781342
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1701
- NMPDR:   fig|264201.1.peg.1701
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- PhylomeDB:   Q6MAH4
- ProtClustDB:   CLSK2762462
- BioCyc:   CPRO264201:PC1701-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31745; Mature: 31745

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDGKRIAEEIQLEIKELIQQSLCRPPCLAVLIVGSHPASQIYVNRKTAACKAVGIASI
CEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCHHHHHEECEEEE
KCELPFTISEEELIREVEKLNVDPQIDGILVQLPLPPHINSNRVNYHIDPQKDVDGFHPL
EEECCCEECHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
NVGKMLIGELDGFLPCTPFGIKTLLERTGIEVCGKHALIIGRSNIVGKPMAALLMQSYPG
CHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCCCCHHHHHHHHCCCC
GNATVTVAHRYTKNLKELCLQADLIIVAIGQPKLITADMVKEGVIIVDVGINKIHDSSKK
CCEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHCCEEEEECCCHHHCCCCCC
NGYQIVGDVDFVNVAPKCAFITPVPGGVGPMTIAMLLYNTLLSYSKSQNLNL
CCEEEEECCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIIDGKRIAEEIQLEIKELIQQSLCRPPCLAVLIVGSHPASQIYVNRKTAACKAVGIASI
CEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCHHHHHEECEEEE
KCELPFTISEEELIREVEKLNVDPQIDGILVQLPLPPHINSNRVNYHIDPQKDVDGFHPL
EEECCCEECHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCC
NVGKMLIGELDGFLPCTPFGIKTLLERTGIEVCGKHALIIGRSNIVGKPMAALLMQSYPG
CHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCCCCHHHHHHHHCCCC
GNATVTVAHRYTKNLKELCLQADLIIVAIGQPKLITADMVKEGVIIVDVGINKIHDSSKK
CCEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHCCEEEEECCCHHHCCCCCC
NGYQIVGDVDFVNVAPKCAFITPVPGGVGPMTIAMLLYNTLLSYSKSQNLNL
CCEEEEECCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA