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The map label for this gene is rf-3 [H]

Identifier: 46447207

GI number: 46447207

Start: 1888990

End: 1890591

Strand: Direct

Name: rf-3 [H]

Synonym: pc1573

Alternate gene names: 46447207

Gene position: 1888990-1890591 (Clockwise)

Preceding gene: 46447206

Following gene: 46447210

Centisome position: 78.24

GC content: 39.26

Gene sequence:

>1602_bases
ATGGCCTTAGAACAAGAACAATTAGCCGAAGCTTGCGAAAAAAGGCGAACTTTCGCCATTATCAGCCACCCGGATGCAGG
CAAAACAACTTTAACTGAAAAGCTTCTTCTATATTCTGGAATGATTCATACAGCAGGAATGGTTCGAGGGCGTAAAGGAC
GCAAAGCTGCAGCTTCCGATTGGATGGCAATGGAACAAGAACGAGGGATTTCCATTACAGCTTCAGCCATGCAATTTACG
TATAATAACACGATTATTAACGTATTAGACACCCCTGGACATGAAGACTTTTCCGAAGACACTTACAGGACTTTGACAGC
TGCTGATTGCGCGATCATGGTTATTGATGCTGCAAAAGGGGTTGAAAGACAAACGCGTAAATTATTTGAAGTTTGCCGAT
TACGTAAAATACCAGTTTTAACATTTATTAATAAAATGGATATGCCAGGCCAAGATCCTTTAGATTTAATGAATGAAGTT
GAAAACGTTTTGCAAATTCACTCTTCTGCCTTTAATTGGCCTATTGGAACTGGTAAAGAGTTTTGTGGTGTACTCAATTG
TGCCACCAAAGAATGTTTATTTTTCACAAAAATAGCAGCTGGTGGAGCTCAAAAAGCTGCAATAACGCGTATTCCCCTTT
ATAATGAAGAAACGAAAGAACGTCTTGGCAAAGAGTATTTTGAAAAGCTTATCCAGGAACTAGAACTTCTTGAAATGGCA
GGGAATCCTTTTTCTCAAAAAGAATTTTTAGCAGGAAATGTCACCCCCATCTTCTTTGCCTCAGCTCTAACCAATTTTGG
AATAGAACCTTTTTTTGATGCCTTTGTGAATTTAGCTCCAGCGCCACACACTCGAATGGCAAATAATGATAAAGGAGAAG
AAATTGAAATTGACCCCGTCCAAACACCATTTAGTGCTTATGTTTTTAAACTTCAGGCTAATATGGATAAAAGGCATCGA
GATAGCATGGCTTTTTTACGCATTTGTTCGGGCCGCTTCGATCGGGATCTTGTCGTCAAACATCATAGACTCGGCAAAGA
AGTTCGTCTTTCAAGGCCCCATGGAATGGTTGCAGGAGAAAGAACAACATTAGATATTGCTTATGCAGGTGATGTAATTG
GGGTGATCAATCCTGGAGTTTTTGCCATTGGCGATACCATCTCACTCACAGGAGGATTTAACTTTAAACCTTTGCCTCAA
TTTCAGCCTGAAATTTTTGCTCGTGTACAACCAAAAGACGTCGGTAAAAGAAAGTCTTTCGATAAAGGTGTTTTGCAATT
AACTGACGAAGGTGCGATTCAATTGCTTCGTCCTTATGATTTAAGTGGGGATTTAATTTTTGCTGCAGTAGGCCAACTTC
AATTCGAAGTCATGCAATATCGTCTCAAAGATGAGTATAGTGTTGAAACAATTATTAATTCTCTCCCTTATCAATGCAGT
GCTTGGCTGATAGGAGATAAAGAGACTTTCAAAAAGCCTACAACAGCGGTCATTGTTCAAGATCGTTTTGATCGACCGAT
TATTTTATTTGGAGAACAGTGGGAAAAACAATATGCCATCAAACAAAATCCTGATCACCAACTAGTAGATATTCTTTCTT
AG

Upstream 100 bases:

>100_bases
AAGAATTAGAATCCAAAGCATAAATCACTACATGTAAAAAATGCTATACAGTGATATACTTTTGCGATTAATTCACATTA
GATTTTAGGAAGCGCATTAT

Downstream 100 bases:

>100_bases
TGCAAACCTGACACAATTTATTAAATACCATATTTTAAAGTTTCTTGGACGCAATACAAAAAAATCGGTGTTTTATAAAT
GTCTTTAGCGTAGTAAATCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MALEQEQLAEACEKRRTFAIISHPDAGKTTLTEKLLLYSGMIHTAGMVRGRKGRKAAASDWMAMEQERGISITASAMQFT
YNNTIINVLDTPGHEDFSEDTYRTLTAADCAIMVIDAAKGVERQTRKLFEVCRLRKIPVLTFINKMDMPGQDPLDLMNEV
ENVLQIHSSAFNWPIGTGKEFCGVLNCATKECLFFTKIAAGGAQKAAITRIPLYNEETKERLGKEYFEKLIQELELLEMA
GNPFSQKEFLAGNVTPIFFASALTNFGIEPFFDAFVNLAPAPHTRMANNDKGEEIEIDPVQTPFSAYVFKLQANMDKRHR
DSMAFLRICSGRFDRDLVVKHHRLGKEVRLSRPHGMVAGERTTLDIAYAGDVIGVINPGVFAIGDTISLTGGFNFKPLPQ
FQPEIFARVQPKDVGKRKSFDKGVLQLTDEGAIQLLRPYDLSGDLIFAAVGQLQFEVMQYRLKDEYSVETIINSLPYQCS
AWLIGDKETFKKPTTAVIVQDRFDRPIILFGEQWEKQYAIKQNPDHQLVDILS

Sequences:

>Translated_533_residues
MALEQEQLAEACEKRRTFAIISHPDAGKTTLTEKLLLYSGMIHTAGMVRGRKGRKAAASDWMAMEQERGISITASAMQFT
YNNTIINVLDTPGHEDFSEDTYRTLTAADCAIMVIDAAKGVERQTRKLFEVCRLRKIPVLTFINKMDMPGQDPLDLMNEV
ENVLQIHSSAFNWPIGTGKEFCGVLNCATKECLFFTKIAAGGAQKAAITRIPLYNEETKERLGKEYFEKLIQELELLEMA
GNPFSQKEFLAGNVTPIFFASALTNFGIEPFFDAFVNLAPAPHTRMANNDKGEEIEIDPVQTPFSAYVFKLQANMDKRHR
DSMAFLRICSGRFDRDLVVKHHRLGKEVRLSRPHGMVAGERTTLDIAYAGDVIGVINPGVFAIGDTISLTGGFNFKPLPQ
FQPEIFARVQPKDVGKRKSFDKGVLQLTDEGAIQLLRPYDLSGDLIFAAVGQLQFEVMQYRLKDEYSVETIINSLPYQCS
AWLIGDKETFKKPTTAVIVQDRFDRPIILFGEQWEKQYAIKQNPDHQLVDILS
>Mature_532_residues
ALEQEQLAEACEKRRTFAIISHPDAGKTTLTEKLLLYSGMIHTAGMVRGRKGRKAAASDWMAMEQERGISITASAMQFTY
NNTIINVLDTPGHEDFSEDTYRTLTAADCAIMVIDAAKGVERQTRKLFEVCRLRKIPVLTFINKMDMPGQDPLDLMNEVE
NVLQIHSSAFNWPIGTGKEFCGVLNCATKECLFFTKIAAGGAQKAAITRIPLYNEETKERLGKEYFEKLIQELELLEMAG
NPFSQKEFLAGNVTPIFFASALTNFGIEPFFDAFVNLAPAPHTRMANNDKGEEIEIDPVQTPFSAYVFKLQANMDKRHRD
SMAFLRICSGRFDRDLVVKHHRLGKEVRLSRPHGMVAGERTTLDIAYAGDVIGVINPGVFAIGDTISLTGGFNFKPLPQF
QPEIFARVQPKDVGKRKSFDKGVLQLTDEGAIQLLRPYDLSGDLIFAAVGQLQFEVMQYRLKDEYSVETIINSLPYQCSA
WLIGDKETFKKPTTAVIVQDRFDRPIILFGEQWEKQYAIKQNPDHQLVDILS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=27.6288659793814, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19923640, Length=514, Percent_Identity=27.431906614786, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI25306283, Length=396, Percent_Identity=29.7979797979798, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=31.9727891156463, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI157426893, Length=161, Percent_Identity=33.5403726708075, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=30.4054054054054, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=127, Percent_Identity=35.4330708661417, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=127, Percent_Identity=35.4330708661417, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=127, Percent_Identity=35.4330708661417, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=470, Percent_Identity=47.2340425531915, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1789738, Length=480, Percent_Identity=27.9166666666667, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=33.9622641509434, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI48994988, Length=182, Percent_Identity=33.5164835164835, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=480, Percent_Identity=27.0833333333333, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=496, Percent_Identity=23.1854838709677, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=155, Percent_Identity=31.6129032258064, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=160, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=148, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=148, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=27.4058577405858, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=514, Percent_Identity=26.6536964980545, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323320, Length=160, Percent_Identity=28.75, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=30, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=30, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582462, Length=498, Percent_Identity=26.5060240963855, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221458488, Length=530, Percent_Identity=25.8490566037736, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI78706572, Length=145, Percent_Identity=33.7931034482759, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=152, Percent_Identity=30.2631578947368, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585713, Length=152, Percent_Identity=30.2631578947368, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585709, Length=152, Percent_Identity=30.2631578947368, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21357743, Length=149, Percent_Identity=28.8590604026846, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574573, Length=166, Percent_Identity=33.7349397590361, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59917; Mature: 59785

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEQEQLAEACEKRRTFAIISHPDAGKTTLTEKLLLYSGMIHTAGMVRGRKGRKAAASD
CCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
WMAMEQERGISITASAMQFTYNNTIINVLDTPGHEDFSEDTYRTLTAADCAIMVIDAAKG
HHHHHHHCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCC
VERQTRKLFEVCRLRKIPVLTFINKMDMPGQDPLDLMNEVENVLQIHSSAFNWPIGTGKE
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FCGVLNCATKECLFFTKIAAGGAQKAAITRIPLYNEETKERLGKEYFEKLIQELELLEMA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GNPFSQKEFLAGNVTPIFFASALTNFGIEPFFDAFVNLAPAPHTRMANNDKGEEIEIDPV
CCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
QTPFSAYVFKLQANMDKRHRDSMAFLRICSGRFDRDLVVKHHRLGKEVRLSRPHGMVAGE
CCCHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
RTTLDIAYAGDVIGVINPGVFAIGDTISLTGGFNFKPLPQFQPEIFARVQPKDVGKRKSF
CEEEEEEECCCEEEECCCCEEEECCEEEECCCCCCCCCCCCCHHHHEECCCHHCCCCCCC
DKGVLQLTDEGAIQLLRPYDLSGDLIFAAVGQLQFEVMQYRLKDEYSVETIINSLPYQCS
CCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEE
AWLIGDKETFKKPTTAVIVQDRFDRPIILFGEQWEKQYAIKQNPDHQLVDILS
EEEECCHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
ALEQEQLAEACEKRRTFAIISHPDAGKTTLTEKLLLYSGMIHTAGMVRGRKGRKAAASD
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
WMAMEQERGISITASAMQFTYNNTIINVLDTPGHEDFSEDTYRTLTAADCAIMVIDAAKG
HHHHHHHCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCC
VERQTRKLFEVCRLRKIPVLTFINKMDMPGQDPLDLMNEVENVLQIHSSAFNWPIGTGKE
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FCGVLNCATKECLFFTKIAAGGAQKAAITRIPLYNEETKERLGKEYFEKLIQELELLEMA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GNPFSQKEFLAGNVTPIFFASALTNFGIEPFFDAFVNLAPAPHTRMANNDKGEEIEIDPV
CCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
QTPFSAYVFKLQANMDKRHRDSMAFLRICSGRFDRDLVVKHHRLGKEVRLSRPHGMVAGE
CCCHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
RTTLDIAYAGDVIGVINPGVFAIGDTISLTGGFNFKPLPQFQPEIFARVQPKDVGKRKSF
CEEEEEEECCCEEEECCCCEEEECCEEEECCCCCCCCCCCCCHHHHEECCCHHCCCCCCC
DKGVLQLTDEGAIQLLRPYDLSGDLIFAAVGQLQFEVMQYRLKDEYSVETIINSLPYQCS
CCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEE
AWLIGDKETFKKPTTAVIVQDRFDRPIILFGEQWEKQYAIKQNPDHQLVDILS
EEEECCHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]