Click here to switch to the map view.

The map label for this gene is cafA [H]

Identifier: 46446951

GI number: 46446951

Start: 1572846

End: 1574384

Strand: Direct

Name: cafA [H]

Synonym: pc1317

Alternate gene names: 46446951

Gene position: 1572846-1574384 (Clockwise)

Preceding gene: 46446947

Following gene: 46446952

Centisome position: 65.14

GC content: 36.71

Gene sequence:

>1539_bases
ATGCACGAAATTTTACTTAACATTGAATCTAAAGAAACTCGCTATGCCCTGCTAAAAAATGGTGAGTTACGTGATCTCGT
CGTCGAACGTAAAAAAGAGCGGCAGCTAACAGGTAATATTTATCGCGGTCGAGTCAAAAACATTCTTCACAATATTCAAT
CTGCTTTCATCGACATTAATGAAGGAGAAAACGGATTTATCCATATCTCTGATATCATTGAAAATACCAAAAAGTTTGAA
CAACTTTTTGATATGGACTTCGACCTCGACTACGATATTAAAGCTTTAAATGAAAAAGAACAGCAAAATTTAGATATTGA
ACAAGTCATGAAACCGGATCAACCGGTCCTAGTTCAAGTTGTCAAGGAACCGATTGGCTCAAAAGGAGCTCGGTTAACTT
CAAATGTTTCTATCGCAGGCCGTTATTTAGTTTTACTTCCTAACTCTTCTCATCGCGGAGTTTCTCGCAAAATTGAAGAT
CGAGTTGCTCGTGAAAGACTAAAAAAACTCATTCGCGCTTTTGAAATGCCACAAGACATGGGCCTAATTTGCCGAACAGC
TAGTGCTAGTGCTACTCAAGAAATGCTCATAGCCGAAGCTCATGACCTTTTGCATAACTGGCAAACAATCATGGAAAACT
TTAAAAAAGCAACAGAACCAACTCTTTTATACGCAGAATCTGATCTTATTAAAAAAGCCGTCATTACCGCCATAGACAAA
CGTTATGACCGCGTTTTAGTTGATGATTATGCCACCTATCAAACTTGTAAACGGCTTTATAGTCGTTATGCCAGCGAACA
TGCTTTACGAATCGAGTATTATCGGGATAAAGTTCCCATGTTTGAACGTTTTAACGTAGAGCGAGAAATTGAAAAAACCT
TACGAAGAAAAATTTGGCTTCCTAGCGGCGGATATCTTTACTTTGATCGTACTGAAGCCATGTACACAATTGATGTTAAC
TCAGGCCGCAGTAATAATAACAAAACTGATGTAGAAGAATCTTTAGTTAGAATTAATTTAGAAGCAGCTGAAGAAATTTC
TAGACAATTACGATTACGCAATATTGGTGGATTAGTCATCTGCGATTTTATCGATATGCGTCTAAGAAAAAATCAACGAC
GTGTTCTAGAAAGATTAAAAGATTGTATGAAAGAAGATTCTGCTAAATGTACAATTTTGGGAATGAGTGAATTCGGCTTA
GTGGAAATGACCAGACAGCGCAATAGAGGCTCTTTATTGCAAACTATTTTTACTGGCTGTCCCTACTGTGCTGGTAGTGG
TATCATCAAATCTCATGAAAGCTTATCTATCGAAATTGAACGAGCTTTCAAAAAAATTGTCGCTTATCACGAACAATTTG
CTCTCAAACTTGTCGTTCATCCTGAATTAGATCGGTATTTAAATATTATTGATAAACAATATTTGTTCAGGCTGGCAACT
GACTATAATGCACATCTTACTTGTGAAGTCGATGATCGCTTACATCTCAATGAATACCAATTTTTTTCGACAATTACCAA
TAAACGAATCGAGGTTTAA

Upstream 100 bases:

>100_bases
TTTCAAGATTTTCTCCTATTAAAAATTGTATGAATTAGCAGAAAGATGAGACAAATAGAAACGTGAACCTGTCTTTCGTC
ATAAAGACCAAGGTAACGAC

Downstream 100 bases:

>100_bases
TTGGGTATGGTCAAACCGATCGTAGATCTTGCTAAGTATCTTGAGAAAGGAATCCTAAAGCCCCCATTCGCAGCAATCAT
TGAACATTTTCATGATAGTT

Product: putative ribonuclease G (axial filament protein)

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MHEILLNIESKETRYALLKNGELRDLVVERKKERQLTGNIYRGRVKNILHNIQSAFIDINEGENGFIHISDIIENTKKFE
QLFDMDFDLDYDIKALNEKEQQNLDIEQVMKPDQPVLVQVVKEPIGSKGARLTSNVSIAGRYLVLLPNSSHRGVSRKIED
RVARERLKKLIRAFEMPQDMGLICRTASASATQEMLIAEAHDLLHNWQTIMENFKKATEPTLLYAESDLIKKAVITAIDK
RYDRVLVDDYATYQTCKRLYSRYASEHALRIEYYRDKVPMFERFNVEREIEKTLRRKIWLPSGGYLYFDRTEAMYTIDVN
SGRSNNNKTDVEESLVRINLEAAEEISRQLRLRNIGGLVICDFIDMRLRKNQRRVLERLKDCMKEDSAKCTILGMSEFGL
VEMTRQRNRGSLLQTIFTGCPYCAGSGIIKSHESLSIEIERAFKKIVAYHEQFALKLVVHPELDRYLNIIDKQYLFRLAT
DYNAHLTCEVDDRLHLNEYQFFSTITNKRIEV

Sequences:

>Translated_512_residues
MHEILLNIESKETRYALLKNGELRDLVVERKKERQLTGNIYRGRVKNILHNIQSAFIDINEGENGFIHISDIIENTKKFE
QLFDMDFDLDYDIKALNEKEQQNLDIEQVMKPDQPVLVQVVKEPIGSKGARLTSNVSIAGRYLVLLPNSSHRGVSRKIED
RVARERLKKLIRAFEMPQDMGLICRTASASATQEMLIAEAHDLLHNWQTIMENFKKATEPTLLYAESDLIKKAVITAIDK
RYDRVLVDDYATYQTCKRLYSRYASEHALRIEYYRDKVPMFERFNVEREIEKTLRRKIWLPSGGYLYFDRTEAMYTIDVN
SGRSNNNKTDVEESLVRINLEAAEEISRQLRLRNIGGLVICDFIDMRLRKNQRRVLERLKDCMKEDSAKCTILGMSEFGL
VEMTRQRNRGSLLQTIFTGCPYCAGSGIIKSHESLSIEIERAFKKIVAYHEQFALKLVVHPELDRYLNIIDKQYLFRLAT
DYNAHLTCEVDDRLHLNEYQFFSTITNKRIEV
>Mature_512_residues
MHEILLNIESKETRYALLKNGELRDLVVERKKERQLTGNIYRGRVKNILHNIQSAFIDINEGENGFIHISDIIENTKKFE
QLFDMDFDLDYDIKALNEKEQQNLDIEQVMKPDQPVLVQVVKEPIGSKGARLTSNVSIAGRYLVLLPNSSHRGVSRKIED
RVARERLKKLIRAFEMPQDMGLICRTASASATQEMLIAEAHDLLHNWQTIMENFKKATEPTLLYAESDLIKKAVITAIDK
RYDRVLVDDYATYQTCKRLYSRYASEHALRIEYYRDKVPMFERFNVEREIEKTLRRKIWLPSGGYLYFDRTEAMYTIDVN
SGRSNNNKTDVEESLVRINLEAAEEISRQLRLRNIGGLVICDFIDMRLRKNQRRVLERLKDCMKEDSAKCTILGMSEFGL
VEMTRQRNRGSLLQTIFTGCPYCAGSGIIKSHESLSIEIERAFKKIVAYHEQFALKLVVHPELDRYLNIIDKQYLFRLAT
DYNAHLTCEVDDRLHLNEYQFFSTITNKRIEV

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=452, Percent_Identity=38.0530973451327, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1787325, Length=444, Percent_Identity=34.6846846846847, Blast_Score=268, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59785; Mature: 59785

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEILLNIESKETRYALLKNGELRDLVVERKKERQLTGNIYRGRVKNILHNIQSAFIDIN
CCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECC
EGENGFIHISDIIENTKKFEQLFDMDFDLDYDIKALNEKEQQNLDIEQVMKPDQPVLVQV
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHCCCHHHHHCCCCHHHHHH
VKEPIGSKGARLTSNVSIAGRYLVLLPNSSHRGVSRKIEDRVARERLKKLIRAFEMPQDM
HHHCCCCCCCEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLICRTASASATQEMLIAEAHDLLHNWQTIMENFKKATEPTLLYAESDLIKKAVITAIDK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
RYDRVLVDDYATYQTCKRLYSRYASEHALRIEYYRDKVPMFERFNVEREIEKTLRRKIWL
HHCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCCHHHHCCCHHHHHHHHHHEECC
PSGGYLYFDRTEAMYTIDVNSGRSNNNKTDVEESLVRINLEAAEEISRQLRLRNIGGLVI
CCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHEEEHHHHHHHHHHHHHHCCCCEEE
CDFIDMRLRKNQRRVLERLKDCMKEDSAKCTILGMSEFGLVEMTRQRNRGSLLQTIFTGC
EHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCC
PYCAGSGIIKSHESLSIEIERAFKKIVAYHEQFALKLVVHPELDRYLNIIDKQYLFRLAT
CCCCCCCCEECCCCEEEEHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHC
DYNAHLTCEVDDRLHLNEYQFFSTITNKRIEV
CCCCEEEEEECCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHEILLNIESKETRYALLKNGELRDLVVERKKERQLTGNIYRGRVKNILHNIQSAFIDIN
CCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECC
EGENGFIHISDIIENTKKFEQLFDMDFDLDYDIKALNEKEQQNLDIEQVMKPDQPVLVQV
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHCCCHHHHHCCCCHHHHHH
VKEPIGSKGARLTSNVSIAGRYLVLLPNSSHRGVSRKIEDRVARERLKKLIRAFEMPQDM
HHHCCCCCCCEEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLICRTASASATQEMLIAEAHDLLHNWQTIMENFKKATEPTLLYAESDLIKKAVITAIDK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
RYDRVLVDDYATYQTCKRLYSRYASEHALRIEYYRDKVPMFERFNVEREIEKTLRRKIWL
HHCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCCHHHHCCCHHHHHHHHHHEECC
PSGGYLYFDRTEAMYTIDVNSGRSNNNKTDVEESLVRINLEAAEEISRQLRLRNIGGLVI
CCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHEEEHHHHHHHHHHHHHHCCCCEEE
CDFIDMRLRKNQRRVLERLKDCMKEDSAKCTILGMSEFGLVEMTRQRNRGSLLQTIFTGC
EHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCC
PYCAGSGIIKSHESLSIEIERAFKKIVAYHEQFALKLVVHPELDRYLNIIDKQYLFRLAT
CCCCCCCCEECCCCEEEEHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHC
DYNAHLTCEVDDRLHLNEYQFFSTITNKRIEV
CCCCEEEEEECCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]